Biomedical Engineering and Bioengineering Commons^™

Automated Detection And Analysis Of Depolarization Events In Human Cardiomyocytes Using Madec, Agnieszka F. Szymanska, Christopher M. Heylman, Rupsa Datta, Enrico Gratton

Biomedical Engineering

Optical imaging-based methods for assessing the membrane electrophysiology of in vitro human cardiac cells allow for non-invasive temporal assessment of the effect of drugs and other stimuli. Automated methods for detecting and analyzing the depolarization events (DEs) in image-based data allow quantitative assessment of these different treatments. In this study, we use 2-photon microscopy of fluorescent voltage-sensitive dyes (VSDs) to capture the membrane voltage of actively beating human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs). We built a custom and freely available Matlab software, called MaDEC, to detect, quantify, and compare DEs of hiPS-CMs treated with the β-adrenergic drugs, propranolol and …