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Full-Text Articles in Biomedical Engineering and Bioengineering
Collagen Fibres Are Not Required For Initial Matrix Mineralization By Bone Cells, M. M. Hosseini, S. A. F. Peel, J. E. Davies
Collagen Fibres Are Not Required For Initial Matrix Mineralization By Bone Cells, M. M. Hosseini, S. A. F. Peel, J. E. Davies
Cells and Materials
Passaged primary cultures of young adult rat bone marrow cells were maintained in medium containing combinations of the supplements dexamethasone, ascorbic acid and Na-ß-glycerophosphate. The cultures were examined by both light and scanning electron microscopy (with additional energy dispersive X-ray analysis). In cultures with fully supplemented medium, an extracellular matrix formed at the culture dish surface, beneath developing bone nodules; this matrix was mineralized but collagen-fibre free and corresponded to that which we have reported previously as the equivalent of the cement line in vivo. In selected samples this interfacial, cement line, matrix was analyzed by X-ray …
A Simple Freeze Fracture Technique For Scanning Electron Microscopy Of Collagenous Biomaterials, David L. Christiansen, George Pins, Frederick H. Silver
A Simple Freeze Fracture Technique For Scanning Electron Microscopy Of Collagenous Biomaterials, David L. Christiansen, George Pins, Frederick H. Silver
Cells and Materials
A simple freeze fracture technique for the ultrastructural analysis of fragile collagenous biomaterials is presented. Following standard methods of preparation and dehydration, fragile biomaterials are fractured with a sharp blade attached to a modified slide hammer. The sliding mass imparts a sudden impact load on the frozen surface of the material , creating a distortion free cleavage plane across the material. More traditional methods including bending and cutting with scissors introduce artifacts indirectly associated with stress concentration produced by bending, and sample compression at the edges with cutting. The impact loading of the sample during fracture results in preservation of …
A Scanning Electron Microscope Study Of The Ultrastructural Organization Of Bone Mineral, F. B. Bagambisa, U. Joos, W. Schilli
A Scanning Electron Microscope Study Of The Ultrastructural Organization Of Bone Mineral, F. B. Bagambisa, U. Joos, W. Schilli
Cells and Materials
Scanning electron microscopy (SEM) was used to study samples of lamellar bone at magnifications typical for the published transmission electron micrographs, to gain more insight into the three-dimensional ultrastructure of bone mineral. Untreated (whole bone) samples allowed an assessment of the degree of mineralization. Deproteinized samples revealed the ultrastructural form and organization of bone apatite to be a function of the extent to which collagen fibers were imbibed with mineral. Numerous parallel formations reminiscent of troughs, gutters, or furrows, pierced and traversed the mineralization front. These troughs showed varying diameters identifiable with collagen fibers, and were separated from one another …
A Model For A Model: Pressure Jackets On Nude Mice With Implants Of Hypertrophic Scars, C. Ward Kischer, Jana Pindur
A Model For A Model: Pressure Jackets On Nude Mice With Implants Of Hypertrophic Scars, C. Ward Kischer, Jana Pindur
Cells and Materials
The hypertrophic scar, a consequence of deep surface injury, is peculiar to humankind. Subcutaneous implants of these tissues in nude mice provide a quasi - animal model for experimental use. An efficacious treatment to promote acceleration of maturation of the hypertrophic scar is controlled topical pressure produced by flexible dacron fabric wraps. Implants in the nude mouse provide a model for study of the effects of pressure. Preliminary results indicate that those implants in nude mice, responding favorably to pressure jackets, show changes consistent with those previously obtained in human studies. The collagen matrix shows reduced density and parallel layering …
Peripheral Nerve Regeneration Within Tubular Prosthesis: Effects Of Laminin And Collagen Matrices On Cellular Ingrowth, Ivar J. Kljavin, Roger D. Madison
Peripheral Nerve Regeneration Within Tubular Prosthesis: Effects Of Laminin And Collagen Matrices On Cellular Ingrowth, Ivar J. Kljavin, Roger D. Madison
Cells and Materials
Bridging a severed peripheral nerve with a tubular conduit, "entubulation repair" , allows manipulation of the microenvironment encountered by regenerating axons. In the present study, we placed the ends of severed mouse sciatic nerves into nonpermeable Tygon tubes to bridge a 4 mm nerve gap. The tube lumen was filled with either a collagen or laminin matrix. The matrices formed a stable 3-D structure connecting both proximal and distal stumps, and provided a scaffolding for the regenerating axons and their supporting cells. We examined with scanning electron microscopy (SEM), transmission electron microscopy (TEM) and light microscopy, the initial events of …