Biomedical Engineering and Bioengineering Commons^™

Using Scratch Wound Assay To Study The Effect Of Soil Arsenic On Human Keratinocyte Cell Migration Due To Contact Exposure, Manas Warke, Laura De March, Srinivas Kannan, Madeline English, Rohan Sarkar, Rupali Datta, Smitha Rao

Michigan Tech Research Data

The scratch wound assay was performed on Human immortalized keratinocytes (HaCaT) cells to observe the effect on cell migration due to contact exposure to arsenic-contaminated Immokalee soil. The cell migration was observed through a microscope for 72 h. HaCaT cells were seeded in 48-well plate. On day 3, treatment media was added (n=8). The cells were treated with four concentrations of soil As (45, 225, 450, and 900 mg/kg) and two controls - Negative control (NC; Pure media) and control (C; 0 mg/kg soil As) for 72 h. A scratch was made using a pipette tip. The wound healing was …

Developing A 3d In Vitro Model By Microfluidics, Hung-Ta Chien

Dissertations and Theses

In vitro tissue models play an important role in providing a platform that mimics the realistic tissue microenvironment for stimulating and characterizing the cellular behavior. In particular, the hydrogel-based 3D in vitro models allow the cells to grow and interact with their surroundings in all directions, thus better mimicking in vivo than their 2D counterparts. The objective of this thesis is to establish a 3D in vitro model that mimics the anatomical and functional complexity of the realistic cancer microenvironment for conveniently studying the transport coupling in porous tissue structures. We pack uniform-sized PEGDA-GelMA microgels in a microfluidic chip to …

Continual Cell Deformation Induced Via Attachment To Oriented Fibers Enhances Fibroblast Cell Migration, Sisi Qin, Vincent Ricotta, Marcia Simon, Richard A. F. Clark, Miriam Rafailovich

Department of Biomedical Engineering Faculty Publications

Fibroblast migration is critical to the wound healing process. In vivo, migration occurs on fibrillar substrates, and previous observations have shown that a significant time lag exists before the onset of granulation tissue. We therefore conducted a series of experiments to understand the impact of both fibrillar morphology and migration time. Substrate topography was first shown to have a profound influence. Fibroblasts preferentially attach to fibrillar surfaces, and orient their cytoplasm for maximal contact with the fiber edge. In the case of en-mass cell migration out of an agarose droplet, fibroblasts on flat surfaces emerged with an enhanced velocity, v …

Creation Of A 3d Construct To Aid Cell Migration And Promote Cell Capture, Joseph Michael Sanders

Legacy Theses & Dissertations (2009 - 2024)

Most cancer-related deaths are attributed to metastasis. The tumor microenvironment is a complex environment which is not fully understood. The Nano Intravital Device (NANIVID) is a versatile, biocompatible device that allows for the manipulation of the tumor microenvironment in vitro and in vivo, providing a platform to study various aspects of tumor progression. The purpose of this study is to modify the NANIVID to resemble the tumor microenvironment in order to allow for a seamless transition from the in vivo environment into the engineered environment within the NANIVID. This engineered microenvironment will promote cell migration and cell capture. It has …

The Mechanotransduction Of Primary Cilia In Tumor Progression Of Lung Adenocarcinoma, Sagar Patel

Theses and Dissertations

The objective of this study was to investigate primary cilia and their mechanotransduction role in lung adenocarcinoma tumor progression. The main focus investigated the effect of primary cilia on cell cycle progression, survival, adhesion and migration analysis of these cells and the role of sonic hedgehog signaling pathway in mechanotransduction. Human Non-Small Cell Lung Cancer (NSCLC) adenocarcinoma biopsies contain more primary cilia than non-tumor lung sections. To observe the effects of primary cilia presence in lung cancer cells in-vitro, formation of primary cilia is inhibited using small interfering RNA. A549 cells with intact primary cilia observe less cell cycle progression …

Quantitative Elucidation Of A Distinct Spatial Gradient-Sensing Mechanism In Fibroblasts, Ian C. Schneider, Jason M. Haugh

Ian C. Schneider

Migration of eukaryotic cells toward a chemoattractant often relies on their ability to distinguish receptor-mediated signaling at different subcellular locations, a phenomenon known as spatial sensing. A prominent example that is seen during wound healing is fibroblast migration in platelet-derived growth factor (PDGF) gradients. As in the well-characterized chemotactic cells Dictyostelium discoideum and neutrophils, signaling to the cytoskeleton via the phosphoinositide 3-kinase pathway in fibroblasts is spatially polarized by a PDGF gradient; however, the sensitivity of this process and how it is regulated are unknown. Through a quantitative analysis of mathematical models and live cell total internal reflection fluorescence microscopy …

Spatial Analysis Of 3′ Phosphoinositide Signaling In Living Fibroblasts, Iii: Influence Of Cell Morphology And Morphological Polarity, Ian C. Schneider, Elizabeth M. Parrish, Jason M. Haugh

Ian C. Schneider

Activation of phosphoinositide (PI) 3-kinase is a required signaling pathway in fibroblast migration directed by platelet-derived growth factor. The pattern of 3′ PI lipids in the plasma membrane, integrating local Pl 3-kinase activity as well as 3′ PI diffusion and turnover, influences the spatiotemporal regulation of the cytoskeleton. In fibroblasts stimulated uniformly with platelet-derived growth factor, visualized using total internal reflection fluorescence microscopy, we consistently observed localized regions with significantly higher or lower 3′ PI levels than adjacent regions (hot and cold spots, respectively). A typical cell contained multiple hot spots, coinciding with apparent leading edge structures, and at most …