Biomedical Engineering and Bioengineering Commons^™

Interaction Of Due-B And Treslin During The Initiation Of Dna Replication, Sumeet Poudel

Browse all Theses and Dissertations

The initiation of DNA replication is a highly regulated and coordinated process. To ensure that the entire genome is replicated only once per cell cycle, many replication proteins are assembled on the chromatin in a step-wise and cell cycle dependent manner. This process is controlled by interaction of replication proteins, post-translational modifications of the replication factors, control of cellular localization of the proteins, or replication factor degradation after their function terminates. Two kinases, CDK (cyclin dependent kinase) and DDK (Dbf4/Drf1 dependent kinase), play important roles during the initiation stage of DNA replication. The c-myc DNA unwinding element-binding protein (DUE-B) is …

Regulation Of The Dna Unwinding Element Binding Protein Due-B In The Cell, Yanzhe Gao

Browse all Theses and Dissertations

Eukaryotic cells have evolved tightly controlled processes that make sure the entire genome gets replicated accurately, once per cell cycle. This tight control is fulfilled by degrading replication proteins after their function is carried out, changing their cellular location or by post-translational modification of replication factors to regulate their function. Phosphorylation events carried out by CDKs (cyclin dependent kinases) and DDKs (Dbf4 dependent kinases) play crucial roles in this regulation.

The DNA unwinding element binding protein (DUE-B) is an essential replication protein that binds to the human c-myc DNA replication origin. In this study, I find that DUE-B is also …