Biomedical Engineering and Bioengineering Commons^™

A Multi-Scale Approach To Study Biochemical And Biophysical Aspects Of Resveratrol On Diesel Exhaust Particle-Human Primary Lung Cell Interaction, Wei Zhang, Qifei Li, Mingjie Tang, Han Zhang, Xiaoping Sun, Sige Zou, Judy L. Jensen, Theodore G. Liou, Anhong Zhou

Biological Engineering Faculty Publications

Diesel exhaust particles (DEPs) are major air pollutants that lead to numerous human disorders, especially pulmonary diseases, partly through the induction of oxidative stress. Resveratrol is a polyphenol that ameliorates the production of reactive oxygen species (ROS) and delays aging-related processes. Herein we studied the cytoprotective effect of resveratrol on DEP-exposed human lung cells in a factorial experimental design. This work investigates biophysical features including cellular compositions and biomechanical properties, which were measured at the single-cell level using confocal Raman microspectroscopy (RM) and atomic force microscopy (AFM), respectively. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and partial least square …