Biomedical Engineering and Bioengineering Commons^™

Preparing Non-Human Primates To Study Hand-Eye Coordination In Frontal Eye Fields (Fef) During Delayed Movement Task, Juliusz Cydzik

McKelvey School of Engineering Theses & Dissertations

ABSTRACT OF THE THESIS Preparing Non-Human Primates to Study Hand-Eye Coordination in Frontal Eye Fields (FEF) During Delayed Movement Task by Juliusz Cydzik Master of Science in Biomedical Engineering Washington University in St. Louis, 2023 Professor Lawrence Snyder, Chair Hand-eye coordination enables humans and non-human primates to use their hands and eyes to perform various tasks. We are interested in coordination at the systems level, where saccades and reaches are encoded. The parietal reach region (PRR), situated at the posterior end of the intraparietal sulcus (IPS) and overlapping portions of the medial intraparietal area (MIP) and V6a, is commonly attributed …

Experimental Assessment Of Mouse Sociability Using An Automated Image Processing Approach, Frency Varghese, Jessica A. Burket, Andrew D. Benson, Stephen I. Deutsch, Christian W. Zemlin

Electrical & Computer Engineering Faculty Publications

Mouse is the preferred model organism for testing drugs designed to increase sociability. We present a method to quantify mouse sociability in which the test mouse is placed in a standardized apparatus and relevant behaviors are assessed in three different sessions (called session I, II, and III). The apparatus has three compartments (see Figure 1), the left and right compartments contain an inverted cup which can house a mouse (called “stimulus mouse”). In session I, the test mouse is placed in the cage and its mobility is characterized by the number of transitions made between compartments. In session II, a …

Segway: A Simple Framework For Unsupervised Sleep Segmentation In Experimental Eeg Recordings, Farid Yaghouby, Sridhar Sunderam

Biomedical Engineering Faculty Publications

Sleep analysis in animal models typically involves recording an electroencephalogram (EEG) and electromyogram (EMG) and scoring vigilance state in brief epochs of data as Wake, REM (rapid eye movement sleep) or NREM (non-REM) either manually or using a computer algorithm. Computerized methods usually estimate features from each epoch like the spectral power associated with distinctive cortical rhythms and dissect the feature space into regions associated with different states by applying thresholds, or by using supervised/unsupervised statistical classifiers; but there are some factors to consider when using them:

• Most classifiers require scored sample data, elaborate heuristics or computational steps not easily …

Computational Assessment Of Neural Probe And Brain Tissue Interface Under Transient Motion, Michael Polanco, Sebastian Bawab, Hangsoon Yoon

Mechanical & Aerospace Engineering Faculty Publications

The functional longevity of a neural probe is dependent upon its ability to minimize injury risk during the insertion and recording period in vivo, which could be related to motion-related strain between the probe and surrounding tissue. A series of finite element analyses was conducted to study the extent of the strain induced within the brain in an area around a neural probe. This study focuses on the transient behavior of neural probe and brain tissue interface with a viscoelastic model. Different stages of the interface from initial insertion of neural probe to full bonding of the probe by astro-glial …

Effect Of High-Energy Ball Milling On The Formation And Micro Structural Features Of Carbonated Chlorapatite Nanopowders

Faculty of Engineering University of Malaya

Carbonated chlorapatite nanopowders (n-CCAp) were synthesized by mechanochemical process from calcite (CaCO3), phosphorus pentoxide (P2O5), and calcium chloride (CaCl2) as raw materials. Results demonstrated that the formation of n-CCAp was influenced strongly by the milling time. At the beginning of milling (up to 15 min), CaCO3 and CaCl2 were the dominant phases, while P2O5 disappeared entirely due to its very high deliquescent nature. With increasing the milling time to 600 min, the progressive mechanochemical reaction was completed which resulted in the formation of nanostructured carbonated chlorapatite. According to the X-ray diffraction data, crystallite size of the product decreased from 24 …

Neural Correlates Of Spectral, Temporal And Spectro-Temporal Modulation, Anusha Mohan

USF Tampa Graduate Theses and Dissertations

Natural sounds are characterized by the distribution of acoustic power over different frequency regions and/or time. This is termed spectral, temporal or spectro-temporal modulation. The auditory system is equipped with banks of filters tuned to different spectral, temporal and spectro-temporal modulation frequencies (SM, TM, STM). The sensitivity of the peripheral system to these modulations can be measured by undertaking a linear systems approach. In addition to understanding the psychophysical sensitivity, studying the neural patterns of their processing is also critical. The current study is an attempt to understand the relationship between the behavioral and neural correlates of spectral, temporal and …

Impact Of Insulin Resistance On Behavioral And Neurochemical Deficits In Db/Db Mice, Ajaykumar Narayan Sharma

Browse all Theses and Dissertations

There is a high comorbidity of type-2 diabetes and neuropsychiatric disorders. However, there is paucity of preclinical research to study this phenomenon. The validity of the db/db mouse as an animal model to study type-2 diabetes and related macrovascular and microvascular complications is well established. The first part of this dissertation was designed to investigate comprehensively the db/db mouse behavior as preclinical evidence of type-2 diabetes related major neurobehavioral complications. Juvenile (5-6 weeks) and adult (10-11 weeks) db/db mice were screened for behavioral depression in forced swim test (FST), psychosis-like symptoms using pre-pulse inhibition (PPI) test, anxiety behavior employing elevated …

Fate And Behavior Of Lead In Soils Planted With Metal-Resistant Species (River Birch And Smallwing Sedge), S. P. Klassen, J. E. Mclean, P. R. Grossl, Ronald C. Sims

Biological Engineering Faculty Publications

Phytoremediation of metal-contaminated soils requires an understanding of the interactions between metal-tolerant plant species and soil chemical properties controlling the bioavailability of metals. We conducted controlled laboratory studies to investigate the effects that river birch (Betula occidentalis Hook.) and smallwing sedge (Carex microptera Mack.) had on the fate and behavior of Pb in a contaminated soil (3000 mg Pb/kg) and tailings (13 000 mg Pb/kg) collected from an abandoned mining site in Utah. Significant Pb accumulation in aboveground tissue was observed in smallwing sedge (≥1000 mg/kg dry wt.) in both the soil and tailings, but Pb was primarily excluded by …

Myasthenia Gravis-Like Syndrome Induced By Expression Of Interferon Gamma In The Neuromuscular Junction., Danling Gu, Lise Wogensen, Nigel A. Calcutt, Chunyao Xia, Simin Zhu, John P. Merlie, Howard S. Fox, Jon Lindstrom, Henry C. Powell, Nora Sarvetnick

Journal Articles: Regenerative Medicine

Abnormal humoral responses toward motor end plate constituents in muscle induce myasthenia gravis (MG). To study the etiology of this disease, and whether it could be induced by host defense molecules, we examined the consequences of interferon (IFN) gamma production within the neuromuscular junction of transgenic mice. The transgenic mice exhibited gradually increasing muscular weakness, flaccid paralysis, and functional disruption of the neuromuscular junction that was reversed after administration of an inhibitor of acetylcholinesterase, features which are strikingly similar to human MG. Furthermore, histological examination revealed infiltration of mononuclear cells and autoantibody deposition at motor end plates. Immunoprecipitation analysis indicated …