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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 3 of 3
Full-Text Articles in Biomedical Engineering and Bioengineering
Dynamic Classification Of Moisture Stress Using Canopy And Leaf Temperature Responses To A Step Changes Of Incident Radiation, Erin E. Stevens, George E. Meyer, Ellen T. Paparozzi
Dynamic Classification Of Moisture Stress Using Canopy And Leaf Temperature Responses To A Step Changes Of Incident Radiation, Erin E. Stevens, George E. Meyer, Ellen T. Paparozzi
Honors Theses
Environmental conditions affect plant productivity and understanding how plants respond to drought stress can be measured in different ways. This study focused on measuring leaf response time to induced water stress. Leaf response time to a step increase and step decrease in radiation was computed for four species of well-watered and water-stressed plants in a controlled environment. The canopy temperature was measured with an infrared thermometer and a thermal imaging camera. Thermal images were analyzed to determine the average temperature of a selected single, unobstructed leaf at the top of the canopy. Both the canopy response time and the single …
Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen
Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen
Department of Chemical and Biomolecular Engineering: Faculty Publications
Gene splicing by fusion PCR is a versatile and widely used methodology, especially in synthetic biology. We here describe a rapid method for splicing two fragments by one-round fusion PCR with a dual-asymmetric primers and two-step annealing (ODT) method. During the process, the asymmetric intermediate fragments were generated in the early stage. Thereafter, they were hybridized in the subsequent cycles to serve as template for the target full-length product. The process parameters such as primer ratio, elongation temperature and cycle numbers were optimized. In addition, the fusion products produced with this method were successfully applied in seamless genome editing. The …
Integration Of Biology, Ecology And Engineering For Sustainable Algal‑Based Biofuel And Bioproduct Biorefinery, James Allen, Serpil Unlu, Yaşar Demirel, Paul N. Black, Wayne R. Riekhof
Integration Of Biology, Ecology And Engineering For Sustainable Algal‑Based Biofuel And Bioproduct Biorefinery, James Allen, Serpil Unlu, Yaşar Demirel, Paul N. Black, Wayne R. Riekhof
Department of Chemical and Biomolecular Engineering: Faculty Publications
Despite years of concerted research efforts, an industrial-scale technology has yet to emerge for production and conversion of algal biomass into biofuels and bioproducts. The objective of this review is to explore the ways of possible integration of biology, ecology and engineering for sustainable large algal cultivation and biofuel production systems. Beside the costs of nutrients, such as nitrogen and phosphorous, and fresh water, upstream technologies which are not ready for commercialization both impede economic feasibility and conflict with the ecological benefits in the sector. Focusing mainly on the engineering side of chemical conversion of algae to biodiesel has also …