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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Blue-Light Induced Biosynthesis Of Ros Contributes To The Signaling Mechanism Of Arabidopsis Cryptochrome, Mohamed A. El-Esawi, Louis David Arthaut, Nathalie Jourdan, Alain D'Harlingue, Justin J. Link, Margaret Ahmad, Carlos F. Martino
Blue-Light Induced Biosynthesis Of Ros Contributes To The Signaling Mechanism Of Arabidopsis Cryptochrome, Mohamed A. El-Esawi, Louis David Arthaut, Nathalie Jourdan, Alain D'Harlingue, Justin J. Link, Margaret Ahmad, Carlos F. Martino
Biomedical Engineering and Sciences Faculty Publications
Cryptochromes are evolutionarily conserved blue light receptors with many roles throughout plant growth and development. They undergo conformational changes in response to light enabling interaction with multiple downstream signaling partners. Recently, it has been shown that cryptochromes also synthesize reactive oxygen species (ROS) in response to light, suggesting the possibility of an alternate signaling mechanism. Here we show by fluorescence imaging and microscopy that H202 and ROS accumulate in the plant nucleus after cryptochrome activation. They induce ROS-regulated transcripts including for genes implicated in pathogen defense, biotic and abiotic stress. Mutant cryptochrome alleles that are non-functional in photomorphogenesis retain the …
Targeting The Bacterial Orisome In The Search For New Antibiotics, Julia E. Grimwade, Alan C. Leonard
Targeting The Bacterial Orisome In The Search For New Antibiotics, Julia E. Grimwade, Alan C. Leonard
Biomedical Engineering and Sciences Faculty Publications
There is an urgent need for new antibiotics to combat drug resistant bacteria. Existing antibiotics act on only a small number of proteins and pathways in bacterial cells, and it seems logical that expansion of the target set could lead to development of novel antimicrobial agents. One essential process, not yet exploited for antibiotic discovery, is the initiation stage of chromosome replication, mediated by the bacterial orisome. In all bacteria, orisomes assemble when the initiator protein, DnaA, as well as accessory proteins, bind to a DNA scaffold called the origin of replication (oriC). Orisomes perform the essential tasks of unwinding …
Delivery Of Antioxidant And Anti-Inflammatory Agents For Tissue Engineered Vascular Grafts, Kenyatta S. Washington, Chris A. Bashur
Delivery Of Antioxidant And Anti-Inflammatory Agents For Tissue Engineered Vascular Grafts, Kenyatta S. Washington, Chris A. Bashur
Biomedical Engineering and Sciences Faculty Publications
The treatment of patients with severe coronary and peripheral artery disease represents a significant clinical need, especially for those patients that require a bypass graft and do not have viable veins for autologous grafting. Tissue engineering is being investigated to generate an alternative graft. While tissue engineering requires surgical intervention, the release of pharmacological agents is also an important part of many tissue engineering strategies. Delivery of these agents offers the potential to overcome the major concerns for graft patency and viability. These concerns are related to an extended inflammatory response and its impact on vascular cells such as endothelial …
Cellular Proteomes Drive Tissue-Specific Regulation Of The Heat Shock Response, Jian Ma, Christopher E. Grant, Rosemary Nadine Plagens, Lindsey N. Barrett, Karen S. Kim Guisbert, Eric Guisbert
Cellular Proteomes Drive Tissue-Specific Regulation Of The Heat Shock Response, Jian Ma, Christopher E. Grant, Rosemary Nadine Plagens, Lindsey N. Barrett, Karen S. Kim Guisbert, Eric Guisbert
Biomedical Engineering and Sciences Faculty Publications
The heat shock response (HSR) is a cellular stress response that senses protein misfolding and restores protein folding homeostasis, or proteostasis. We previously identified an HSR regulatory network inCaenorhabditis elegans consisting of highly conserved genes that have important cellular roles in maintaining proteostasis. Unexpectedly, the effects of these genes on the HSR are distinctly tissue-specific. Here, we explore this apparent discrepancy and find that muscle-specific regulation of the HSR by the TRiC/CCT chaperonin is not driven by an enrichment of TRiC/CCT in muscle, but rather by the levels of one of its most abundant substrates, actin. Knockdown of actin subunits …