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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Patient-Derived Hydrogel As A Sacrificial Matrix For Efficient Cell Loading, Shalmli U. Joshi
Honors Scholar Theses
The field of tissue engineering focuses on delivering patient-derived stem cells to the body through the use of degradable biomaterials, such as hydrogels, which are infused into engineered scaffolds. Hydrogels act as templates to support and guide cells towards the regeneration of new tissue. In this study, we introduce a completely intraoperative procedure for obtaining a fibrin hydrogel for tissue engineering applications. In the past, fibrin hydrogel has been commonly formed by combining fibrinogen protein with animal-derived thrombin. Instead, we have developed an automated, and therefore reproducible, protocol to isolate and form fibrin hydrogel without the use of animal-derived thrombin. …
The Agile Approach In Pharmaceutical Software Development, Priyal Bhargava
The Agile Approach In Pharmaceutical Software Development, Priyal Bhargava
Honors Scholar Theses
The Agile development model is increasingly being adopted by the pharmaceutical industry for software development. Currently, there are several software projects under development by pharmaceutical companies that are using the Agile process. Other methodologies such as the Waterfall process, a traditional approach to project management, have evolved over time and been compared against the Agile method. Also there are conflicts of interest which should be taken into consideration when in a pharmaceutical setting such as abiding by FDA regulations. In addition, Agile’s sub-divisional approaches add to the versatility of how this iterative approach is applied in the pharmaceutical industry. The …
Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, Thomas C. Kinard
Engineering A Fluorescent Protease Sensor For In Vivo Protein Detection, Thomas C. Kinard
Honors Scholar Theses
This report details the results of an ongoing project to engineer a mutant form of Red Fluorescent Protein (RFP) variant mCherry that acts as a real-time in vivo protease sensor. The sought-after mutant only becomes fluorescent when exposed to Tobacco Etch Virus (TEV) Protease, this system’s model protease. This will be accomplished via the insertion of the TEV Protease Recognition Site (TEV-PRS) in such a position that, before cleavage, will prevent the protein from folding to fluorescent conformation, but upon cleavage, will allow for fluorescent conformation to occur. The cylindrical structure of the protein, composed of beta-pleated sheets, contains “loops” …
A Stationary Camera For Use With Other Implantable Medical Devices, Ariane Garrett
A Stationary Camera For Use With Other Implantable Medical Devices, Ariane Garrett
Holster Scholar Projects
I designed an optical system for analysis of implantable devices. The final system consists of a 3mm double convex lens that focuses a light spot onto a tissue or implantable medical device. This light spot can then provide feedback on the movement of flow through a device or the development of tissue on the device, although my project focused solely on the flow application. The light spot is generated by an LED and directed using a half mirror. It is then focused using a ball lens, fiber optic, and the double convex lens. With this system the generated light spot …