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Full-Text Articles in Biomedical Engineering and Bioengineering
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover
Biomedical Engineering
Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling …
Esem Analysis Of Mice Femurs With Varying Sost Levels, Patrick K. Mcclay
Esem Analysis Of Mice Femurs With Varying Sost Levels, Patrick K. Mcclay
Biomedical Engineering
This project’s goal was to analyze the properties of the cortical femur on 100 mice bones from Lawrence Livermore National Laboratory. Analysis was limited to imaging which determined the ratio of bone volume to total volume (BV/TV) and osteocyte lacunae density. Mice were altered to knock out their SOST gene: a negative regulator of bone formation. Twelve groups were created to differentiate their treatment, duration, and phenotype. Transgenic (TG) mice had an overexpression of the SOST gene: they carried a bacterial artificial chromosome. Mice with limb defects (DEF) were the offspring of two TG mice and carried twice the amount …