Biomedical Engineering and Bioengineering Commons^™

Detachment Of Human Endothelial Cells Under Flow From Wettability Gradient Surfaces With Different Functional Groups, T. G. Ruardy, H. E. Moorlag, J. M. Schakenraad, H. C. Van Der Mei, H. J. Busscher

Cells and Materials

In this study, the position bound adhesion, spreading and detachment under flow of human umbilical cord endothelial cells (HUVEC) was studied on a dichlorodimethylsilane (DDS), dimethylocta-decylchlorosilane (DOCS) and tridecafluor-1,1,2,2-tetrahydrooctyl-1-dimethylchlorosilane (TFCS) wettability gradient on glass. Gradient surfaces were prepared by the diffusion method and characterized by the Wilhelmy plate technique for their wettability and by X-ray photoelectron spectroscopy (XPS) for their chemical composition. Quantitative analysis of the cellular response on the wettability gradient surfaces showed that the position bound cellular response was influenced by wettability for each type of gradient in a different way. On DDS-wettability gradients, cells withstood flow best …