Biomedical Engineering and Bioengineering Commons^™

Development Of A Bovine X Chromosome Linkage Group And Painting Probes To Assess Cattle, Sheep, And Goat X Chromosome Segment Homologies., Sakthikumar Ambady, F.Abel Ponce Leon, Gregory Hawkins, Steven Kappes, Michael Bishop, James Robl, Craig Beattle

Sakthikumar Ambady

The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes. These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification. The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) …