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Full-Text Articles in Biomedical Engineering and Bioengineering
Intensification Of Clostridium Pasteurianum Fermentation Producing N-Butanol From Glycerol Using Microfiltration Cell Recycle, Colin W. Couper
Intensification Of Clostridium Pasteurianum Fermentation Producing N-Butanol From Glycerol Using Microfiltration Cell Recycle, Colin W. Couper
Electronic Thesis and Dissertation Repository
This work demonstrates lab scale intensification of the fermentation of glycerol to 1-butanol using Clostridum pasteurianum, starting with simulation and comparison of different cell recycle arrangements, development of a cell recycle apparatus with an existing bioreactor, and demonstration of fermentation with the final system. Fermentations performed with the completed system showed that the cell recycle system was not significantly inhibitory to fermentation, and achieved a maximum apparent cell dry weight of 3.14g/L and a maximum butanol productivity of 1.16g/Lh.
Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr
Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr
Electronic Thesis and Dissertation Repository
Two bioprocesses were separately investigated based on their common interest of using gaseous substrates that have low solubility. The first process involved the development of a mixed culture using two organisms capable of utilizing glycerol and carbon monoxide separately to increase biobutanol production, while the second process involved an investigation of different production media used in aerobic xanthan production of Xanthomonas campestris with pressurization effects. It was determined that Clostridium pasteurianum should be used with an organism like Clostridium carboxidivorans or Eubacterium limosum in order for butyrate uptake at 3 g/L to occur with a minimum 0.1 g/L butyrate production. …
Optimization Of Wastewater Microalgae Pretreatment For Acetone, Butanol, And Ethanol Fermentation, Yessica A. Castro
Optimization Of Wastewater Microalgae Pretreatment For Acetone, Butanol, And Ethanol Fermentation, Yessica A. Castro
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Acetone-butanol-ethanol (ABE) fermentation from wastewater microalgae by Clostridium saccharoperbutylacetonicum N1-4 is a novel bioprocess that utilizes waste substrate to generate valuable solvents. Butanol, the most abundant product resulting from ABE fermentation, is an environmentally safe and high performing fuel that can be utilized as a drop-in-fuel; however, high operational costs and low ABE yield present challenge in scale-up of the process. The utilization of algae as a substrate requires pretreatment prior to fermentation to increase the bioavailability of the algal fermentable sugars and to improve the conditions of the pre-fermentation medium. The purpose of this thesis was to optimize wastewater …