Biomedical Engineering and Bioengineering Commons^™

Calcium Phosphate-Based Resorbable Biomaterials For Bone Regeneration, Daniel O. Costa

Electronic Thesis and Dissertation Repository

Bone defects are a prevalent problem in orthopedics and dentistry. Calcium phosphate-based coatings and nanocomposites offer unique solutions towards producing scaffolds with suitable physical, mechanical and biological properties for bone regeneration.

We developed a novel method to synthesize hydroxyapatite (HA) particles with high aspect ratio using sol-gel chemistry and hydrothermal treatment. We obtained tunable pure-phase carbonated-HA in the form of micro/nanorods and nanowires (diameters 25-800 nm). To mimic the structure of bone, HA nanowires were homogenously mixed within poly(ε-caprolactone) (PCL) to produce nanocomposites with improved mechanical properties as determined by uniaxial tensile testing.

Surface chemistry and topography of biomaterials play …

Bacterial Cellulose Templates For Nano-Hydroxyapatite Fibre Synthesis, Jordan A. Demello

Electronic Thesis and Dissertation Repository

Guided bone regeneration is a medical procedure which induces in vivo re-growth of bone using membranes and osteopromoting fillers. In this work, bacterial cellulose fibers were isolated and used as a basis for biomimetic hydroxyapatite growth, with the ultimate goal of producing GBR filler materials. Acetobacter xylinum generated BC using various carbon sources. Fibers were treated with phosphoric acid to phosphorylate functional groups and preconditioned with calcium to nucleate the HA. Simulated body fluid (SBF) furthered the growth. Over 14 days, the product was characterized via EDX, SEM, FTIR, and XRD. The effect of media composition, phosphorylation time, pretreatment, and …

Bifunctional Bisphosphonates For Delivering Biomolecules To Bone, Jivan N. Yewle

Theses and Dissertations--Chemistry

Active targeting with controlled delivery of therapeutic agents to bone is an ideal approach for treatment of several bone diseases. Since bisphosphonates (BPs) are known to have high affinity to bone mineral and are being widely used in treatment of osteoporosis, they are well-suited for drug targeting to bone. For this purpose, bifunctional hydrazine-bisphosphonates (HBPs) with spacers of various lengths and lipophilicity were synthesized and studied. Crystal growth inhibition assays demonstrated that the HBPs with shorter spacers bound more strongly to bone mineral, hydroxyapatite (HA), than did alendronate. HBPs were also demonstrated to be non-toxic to MC3T3-E1 pre-osteoblasts. The targeted …