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Full-Text Articles in Biomedical Engineering and Bioengineering
The Application Of Innovative High-Throughput Techniques To Serum Biomarker Discovery, Izabela Debkiewicz Karbassi
The Application Of Innovative High-Throughput Techniques To Serum Biomarker Discovery, Izabela Debkiewicz Karbassi
Theses and Dissertations in Biomedical Sciences
Time-of-flight mass spectrometry continues to evolve as a promising technique for serum protein expression profiling and biomarker discovery. As seen in our initial SELDI-TOF MS and MALDI-TOF MS profiling study of serum for the assessment of breast cancer risk, many profiling strategies typically employ single chemical affinity beads or surfaces to decrease sample complexity of dynamic fluids like serum. However, most proteins, captured on a particular surface or bead, are not resolved in the lower mass range where mass spectrometers are most effective. To this end we have designed an expression profiling workflow that utilizes immobilized trypsin paramagnetic beads in …
Competitive Enzyme Immunoassay By Polyclonal Antibodies Against Organophosphorus Pesticides, Kuo-Lan Wen
Competitive Enzyme Immunoassay By Polyclonal Antibodies Against Organophosphorus Pesticides, Kuo-Lan Wen
Theses and Dissertations in Biomedical Sciences
Organophosphorus compounds (OPs) comprise one of the major classes of pesticides in use today because they are highly effective and less toxic. Because of their extensive use, there is a need for monitoring OPs in water and vegetables. Gas chromatography (GC) is the usual method for OPs screening, but it is time-consuming and has the high cost of instrumentation. The goal of this project was to find an easy method which was sensitive and had less instrumentation required to test OPs.
Microwells and microtubes were utilized as the solid-phase immobilization for the development of an enzyme immunoassay for OPs. A …