Biomedical Engineering and Bioengineering Commons^™

Quantitation And Identification Of Protein S-Nitrosylation: Implication For The Progression Of Alzheimer's Disease, Siyang Wang

Doctoral Dissertations

Protein S-nitrosylation, the covalent modification of a cysteine sulfhydryl group by nitric oxide (NO), plays a critical role in post-translational modification (PTM) that regulates a large variety of cellular functions and signalling events. The nitrosylation state changes with oxidative damage and is involved in variety of cancers and neurodegeneration diseases.

Three technologies were developed for nitrosylated protein detection and identification. Capillary gel electrophoresis with laser induced fluorescence (CGE-LIF) detection was used for the detection and quantitation of nitrosylated proteins. A two-dimensional micro-electrophoresis (2D μ-CE) separations system was also built to detect nitrosylated proteins via poly (methylmethacrylate) microchips. Proteomics following nitrosylated …

The Contribution Of Oxidative Stress In The Protein Damage And Dna Lesion In Alzheimer's Disease Neuropathology, Cheng Zhang

Doctoral Dissertations

Glutathione (GSH) plays an essential role in the intracellular antioxidant defense against the oxidant radicals, especially the ·OH radical. To understand the early and progressive cellular changes in Alzheimer's disease (AD) development, we investigated reduced glutathione/oxidized glutathione (GSH/GSSG) status in a double mutated AD transgenic mouse model (B6.Cg-Tg), which carries Swedish amyloid precursor protein mutation (APPswe) and exon 9 deletion of the PSEN1 gene. Likewise, S-glutathionylation (Pr-SSG) is a specific post-translational modification (PTM) of cysteine residues by the addition of glutathione. S-glutathionylated proteins induced by oxidative stress play an essential role in understanding the pathogenesis of the aging …