Biomedical Engineering and Bioengineering Commons^™

Abbott Cardiac Electrophysiology Wet Lab Project, Melissa Kurani, Fiona Lynn

Biomedical Engineering: Graduate Reports and Projects

Cardiac mapping systems provide electrophysiologists with pertinent information about ablation treatment plans for patients who suffer from cardiac arrhythmias. This thesis describes the process of designing a functional wet lab that integrates with Abbott’s EnSite Precision 3D Mapping System, with the purpose of providing Cal Poly students and faculty with an opportunity to have a hands-on learning experience with cardiac mapping. This project encompassed a thorough literature review of cardiology, electrophysiology, and in vitro lab systems, followed by the design, manufacturing, and evaluation of a functional and anatomically representative wet lab. This is a continuation of previous master’s projects that …

Abbott Lab Instrumentation Validation, Brandon James Mukai, Bosung Josh Kim

Biomedical Engineering: Graduate Reports and Projects

Over the past 40 years, since the initial catheter ablation procedure was completed, cardiac mapping has become an essential part of electrophysiological procedures. Cardiac mapping is an electrophysiological study that allows physicians to analyze the electrical activity of the heart in order to diagnose and treat cardiovascular disease. Institutions like Cal Poly can utilize professional instrumentation systems with an appropriate wet lab to develop new devices and study various phenomena in an in-vitro physiological environment. Cardiac mapping systems used in tandem with catheter ablation procedures are essential to ensuring that the trend of deaths due to cardiovascular disease continues to …

Analysis Of Electroanatomic Mapping System Accuracy Using X-Ray Reconstruction Of Electrode Locations In A Porcine Animal Model, Hana Boudlali

Master's Theses

Fluoroscopy is considered the gold standard for locating catheters during cardiac electrophysiology (EP) procedures. However, fluoroscopy emits ionizing radiation which can lead to adverse health effects when exposed to in high doses (World Health Organization, 2016). Electroanatomic mapping (EAM) systems display the three-dimensional location of EP catheters and measure the local electrical activity of the heart. They can minimize a physician’s reliance on fluoroscopy and can help reduce radiation exposure during a case (Casella, 2011).

EAM systems are diagnostic medical devices that inform the placement of ablation therapy and must accurately locate catheters to be deemed safe. Test methods to …

Esophageal Temperature Mapping And Monitoring Sheath, Nicole M. Rogers, Hillary Johnson, Alexander Silva

Biomedical Engineering

The purpose of this document is to provide a product development summary for an accessory device that joins an esophageal temperature probe and a mapping catheter together to be used during cardiac ablation. Catheter ablation therapy is performed in the left atrium, utilizing extremely hot or cold temperatures to create scar tissue and treat atrial fibrillation and other arrhythmias. The close positioning of the left atria adjacent to the esophagus presents a large risk as the extreme temperatures used during ablation can lead to esophageal damage or fistulas. This device is designed to integrate correlate data from the temperature probe …

Interfacing A Hirudo Medicinalis Retzius Cell With Insulated Gate Of Mosfet, Rachel M. Smith

Master's Theses

Much work has been done to study the external stimulation of nervous tissue as well as the transmission of neural signals to electronics. Peter Fromherz was one of the pioneers in this area of electrophysiology, with a series of experiments in the 1990s that aimed to characterize and optimize the interface between neural tissue and transistors. In this thesis, Kurt Sjoberg and I interfaced a Retzius cell isolated from a Hirudo medicinalis ganglion with the insulated gate of a MOSFET. The goal was to see change in membrane potential that could be related Fromherz’s original 1991 work. Our experimental setup …

Action Potential Simulation Of The Hirudo Medicinalis's Retzius Cell In Matlab, Zechari Ryan Tempesta

Master's Theses

Modification of Hodgkin and Huxley’s experimentally derived set of nonlinear differential equations was implemented to accurately simulate the action potential of the Hirudo Medicinalis’s Retzius cell in MATLAB under analogous conditions to those found in the Retzius cell environment. The voltage-gated sodium and potassium channel responses to changes in membrane potential, as experimentally determined by Hodgkin and Huxley, were manipulated to suit simulation parameters established by electrophysiological Retzius cell recordings. Application of this methodology permitted additional accurate simulation of the Hirudo Medicinalis’s P cell under analogous conditions to those found in the P cell environment. Further refinement of this technique …

Implementation Of Medicinal Leech Preparation To Investigate The Connection Between The Motor Neuron And Muscle Fiber Via Sharp Electrode Electrophysiology, Chandra Nikole Miller

Master's Theses

There are forty registered organophosphates in the United States and they range from pesticides and insecticides to nerve agents or neurotoxins such as sarin. Organophosphates (OP’s) have been used in chemical warfare for years and tend to lead to death due to an attack on the nervous system. Chemical assays and mass microscopy have been used to assess the concentration of OP’s in the environment, but both methods require the body to metabolize the OP first, which can be detrimental to the victim. It is crucial to come up with a method to investigate and detect these neurotoxins without causing …