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Full-Text Articles in Biomedical Engineering and Bioengineering

Effect Of Fa Chain Length On Normal- And Reversed-Phase Hplc Of Phospholipids, Dongmei Zhai, Peter J. Reilly Dec 2002

Effect Of Fa Chain Length On Normal- And Reversed-Phase Hplc Of Phospholipids, Dongmei Zhai, Peter J. Reilly

Chemical and Biological Engineering Publications

Forty-seven saturated synthetic diacyl PA, PC, PE, PG, and PS and five unsaturated diacyl phospholipids (PL) underwent normal- and reversed-phase (RP) HPLC with isocratic isopropanol/hexane/water (5∶4∶1) and methanol/chloroform/acetonitrile/water (79.5∶9∶8∶3.5) mobile phases, respectively. For normal-phase HPLC, capacity factors (k′ i ) decrease with chain length (n) of the two identical PL FA residues, whereas the opposite occurs with RP (C18)-HPLC. Plots of In k′ i vs. n for individual PL classes are in general curved, violating the linear free-energy relationship. For PL of the same n but with ...


Fermentation Of Cottonseed And Other Feedstuffs In Cattle Rumen Fluid, Ian C. Schneider, Michael L. Ames, Mark A. Rasmussen, Peter J. Reilly Mar 2002

Fermentation Of Cottonseed And Other Feedstuffs In Cattle Rumen Fluid, Ian C. Schneider, Michael L. Ames, Mark A. Rasmussen, Peter J. Reilly

Chemical and Biological Engineering Publications

Bovine rumen fluid was fermented anaerobically over 48 h with cottonseed, corn, alfalfa, or a mixture of these substrates in anaerobic mineral buffer. Samples taken at different incubation times were derivatized with n-butanol and subjected to gas chromatography and mass spectroscopy. No unusual fermentation end-products from the cottonseed substrate were detected. Cottonseed supported rumen fermentation at levels comparable to those of the other substrates. Major components were usually found in the decreasing order of acetate, propionate, butyrate, and valerate, although acetate and propionate concentrations decreased late in the alfalfa and mixed-feed fermentations, eventually allowing butyrate concentrations to exceed those ...


Synergistic Effects Of Physical And Chemical Guidance Cues On Neurite Alignment And Outgrowth On Biodegradable Polymer Substrates, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada Jan 2002

Synergistic Effects Of Physical And Chemical Guidance Cues On Neurite Alignment And Outgrowth On Biodegradable Polymer Substrates, Cheryl Miller, Srdija Jeftinija, Surya K. Mallapragada

Chemical and Biological Engineering Publications

This article demonstrates that directional outgrowth of neurites is promoted by applying a combination of physical and chemical cues to biodegradable polymer substrates. Films of poly-D,L-lactic acid and poly(lactide-co-glycolide) were micropatterned to form grooves on substrate surfaces, using novel indirect transfer techniques developed specifically for biodegradable polymers that cannot be micropatterned directly. Laminin was selectively adsorbed in the grooves. Whole and dissociated dorsal root ganglia were seeded on the substrates and neurite outgrowth and alignment along the microgrooves were measured. The microgrooves provide physical guidance, whereas laminin provides chemical cues to the neurons. The groove depth and spacing ...


Mm# Modeling Of Aldopentose Pyranose Rings, Michael K. Dowd, William M. Rockey, Alfred D. French, Peter J. Reilly Jan 2002

Mm# Modeling Of Aldopentose Pyranose Rings, Michael K. Dowd, William M. Rockey, Alfred D. French, Peter J. Reilly

Chemical and Biological Engineering Publications

MM3 (version 1992, ϵ=3.0) was used to study the ring conformations of d-xylopyranose, d-lyxopyranose and d-arabinopyranose. The energy surfaces exhibit low-energy regions corresponding to chair and skew forms with high-energy barriers between these regions corresponding to envelope and half-chair forms. The lowest energy conformer is 4 C 1 for α- and β-xylopyranose and α- and β-lyxopyranose, and the lowest energy conformer is 1 C 4 for α- and β-arabinopyranose. Only α-lyxopyranose exhibits a secondary low-energy region (1 C 4) within 1 kcal/mol of its global minimum. Overall, the results are in good agreement with NMR and crystallographic ...