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University of Colorado, Boulder

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Articles 1 - 30 of 92

Full-Text Articles in Biomedical Engineering and Bioengineering

Ice-Binding Protein From Shewanella Frigidimarinas Inhibits Ice Crystal Growth In Highly Alkaline Solutions, Elizabeth Delesky, Shane D. Frazier, Jaqueline D. Wallat, Kendra L. Bannister, Chelsea Marie Heveran, Wil V. Srubar Iii Feb 2019

Ice-Binding Protein From Shewanella Frigidimarinas Inhibits Ice Crystal Growth In Highly Alkaline Solutions, Elizabeth Delesky, Shane D. Frazier, Jaqueline D. Wallat, Kendra L. Bannister, Chelsea Marie Heveran, Wil V. Srubar Iii

University Libraries Open Access Fund Supported Publications

The ability of a natural ice-binding protein from Shewanella frigidimarina (SfIBP) to inhibit ice crystal growth in highly alkaline solutions with increasing pH and ionic strength was investigated in this work. The purity of isolated SfIBP was first confirmed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion chromatography with an ultraviolet detector (SEC-UV). Protein stability was evaluated in the alkaline solutions using circular dichroism spectroscopy, SEC-UV, and SDS-PAGE. SfIBP ice recrystallization inhibition (IRI) activity, a measure of ice crystal growth inhibition, was assessed using a modified splat assay. Statistical analysis of results substantiated that, despite partial denaturation and ...


Diagnostics And Selective Nano-Therapeutics To Combat Multidrug-Resistant Bacteria, Partha Pratim Chowdhury Jan 2019

Diagnostics And Selective Nano-Therapeutics To Combat Multidrug-Resistant Bacteria, Partha Pratim Chowdhury

Chemical & Biological Engineering Graduate Theses & Dissertations

Due to extensive use of antibiotics various strains multidrug resistant bacteria are emerging rapidly. In USA alone according to CDC report 2013 more than 2 million is affected by these strains of multidrug resistant bacteria causing several deaths. Hence an effective way of diagnostics and therapeutics need to be designed which can successfully combat the menace of

multidrug drug resistant bacteria.

In the present thesis, we discuss about novel strategies for both diagnostics and therapeutics for multidrug resistant bacteria. In the 1st chapter we discuss a novel DNA sequencing technique utilizing purely optical spectroscopy techniques like surface enhanced Raman and ...


Computational Protein Design For Peptide-Directed Bioconjugation, Joseph Gabriel Plaks Jan 2019

Computational Protein Design For Peptide-Directed Bioconjugation, Joseph Gabriel Plaks

Chemical & Biological Engineering Graduate Theses & Dissertations

Proteins enable living organisms to perform many of their critical functions, having been applied over evolutionary time to solve problems of overwhelming diversity and complexity. Protein engineering seeks to deploy these versatile molecules in addressing problems of human concern and would benefit from innovations that improve protein utilization in unnatural environments as well as from increased predictive capability in protein design.

Bioconjugation facilitates the use of proteins in unnatural environments by permitting the attachment of molecules, such as polymers, that can modulate protein stability, solubility, and activity and by mediating protein immobilization. We initially explored this propensity of bioconjugation by ...


Feasibility And Analysis Of A Hybrid Spacesuit Architecture For Planetary Surface Exploration, Roger Huerta I Lluch Jan 2019

Feasibility And Analysis Of A Hybrid Spacesuit Architecture For Planetary Surface Exploration, Roger Huerta I Lluch

Aerospace Engineering Sciences Graduate Theses & Dissertations

This Thesis presents a novel hybrid spacesuit architecture concept to enable planetary exploration by combining mechanical counterpressure (MCP) with gas-pressurization (GP). This conceptual design has the potential to offer a quantifiable increase in mobility, increases in safety through pressure layer redundancy, and decreased pre-breathe time to improve operational efficiency.

The Thesis performs an engineering study on the concept. First, to assess the feasibility and benefit of implementing two separate pressure layers in the spacesuit, a trade analysis of the design space was performed by numerically modelling different spacesuit-related parameters. The analysis is done considering the combined system performance across different ...


Development Of Novel Crispr-Based Methods For Transcriptional Control Over Bacterial Gene Expression, Katia Tarasava Jan 2019

Development Of Novel Crispr-Based Methods For Transcriptional Control Over Bacterial Gene Expression, Katia Tarasava

Materials Science & Engineering Graduate Theses & Dissertations

One of the challenges of the 21st century is creating a sustainable economy that is able to serve the needs of a growing population with the decreasing supply of natural resources. In addition to alternative energy sources, renewable economy concerns production of materials and chemicals. The chemical and polymer industries rely heavily on petroleum for manufacturing consumer goods. Many of these chemicals can be produced from renewable resources using microorganisms. However, the amounts produced are often not enough to make the process commercially viable. By rewiring cellular metabolism and regulation, it is possible to redirect metabolic flux to increase the ...


Foodlocker: An Online Marketplace For Automated Meals, Josiah Buxton Jan 2019

Foodlocker: An Online Marketplace For Automated Meals, Josiah Buxton

Computer Science Graduate Theses & Dissertations

Food waste is a major problem for the human race, particularly in "rich" industrial countries.

We propose a creative, partial solution to this problem through the development of an online marketplace for left-over food and a physical infrastructure to secure the food throughout each transaction.

The software platform will consist of three separate portions: a mobile food consumer application, a mobile food provider application, and a web food provider application. Restaurants and other licensed food distributors can utilize the food provider applications to broadcast available left-over meals, which can be purchased at a discounted price. The hardware infrastructure will be ...


Nanoscale Stiffness Cues Influence Valvular Interstitial Cell Activation To Myofibroblasts, Michaela Wenning Jan 2019

Nanoscale Stiffness Cues Influence Valvular Interstitial Cell Activation To Myofibroblasts, Michaela Wenning

Undergraduate Honors Theses

Surgery is currently the primary treatment option for aortic valve stenosis (AVS) patients, many of whom are ineligible for surgery and are left untreated. AVS is progression is known to differ between males and females, and an understanding of sex-specific mechanisms of disease progression is imperative in developing accurate treatment options for men and women. The development of a nonsurgical therapy for AVS patients requires a deeper understanding of the molecular and cellular mechanisms of AVS progression. Currently, the role of calcium phosphate nanoparticles detected in the aortic valve during early stages of AVS in influencing disease progression and valvular ...


Thermal Fusion For Sutureless Closure: Devices, Composition, Methods, Eric Anthony Kramer Jan 2018

Thermal Fusion For Sutureless Closure: Devices, Composition, Methods, Eric Anthony Kramer

Mechanical Engineering Graduate Theses & Dissertations

As minimally invasive surgical techniques progress, the demand for reliable ligation is pronounced. The surgical advantages of energy-based vessel sealing exceed those of traditional, compression-based ligatures in procedures sensitive to duration, foreign bodies, and recovery time alike. While the use of energy-based devices to seal or transect vasculature and connective tissue bundles is widespread, the breadth of heating strategies and energy dosimetry used between devices underscores an uncertainty as to the molecular nature of the sealing mechanism and induced tissue bond. Further, energy-based techniques (e.g., tissue “fusion” or tissue “welding”) exhibit promise for the closure, repair and functional recovery ...


Engineering Bacterial Gene Expression: Applications Towards Biofuels And Antibiotic Resistance, Peter Britton Otoupal Jan 2018

Engineering Bacterial Gene Expression: Applications Towards Biofuels And Antibiotic Resistance, Peter Britton Otoupal

Chemical & Biological Engineering Graduate Theses & Dissertations

Nature is rarely static. Organisms live in diverse and stressful environments that necessitate rapid response strategies for survival. Microorganisms have responded to this by evolving bet-hedging, wherein they exhibit constitutively heterogeneous gene expression to maximize fitness across numerous background. The goal of this thesis is to “hijack” this phenomenon using novel gene expression engineering techniques to alter how bacteria respond to their environments, in order to address pressing societal concerns.

This begins with a systematic exploration of how bacterial gene expression naturally responds to antibiotics and biofuels. This reveals promising gene candidates for targeted manipulation, for which a library of ...


The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret Jan 2018

The Role Of Protein-Protein Interactions In Inducing Interfacial Aggregation, Lea Line Gisele Sorret

Chemical & Biological Engineering Graduate Theses & Dissertations

The synergic exposure to silicone oil-water interfaces and to agitation has been shown to promote the aggregation of therapeutic proteins. Silicone oil is typically used as a lubricant for manufacturing surfaces and pharmaceutical containers such as glass prefilled syringes. This is a problem as protein aggregates formed at the silicone oil-water interface may shed into bulk solutions, leading to product recalls or adverse immunogenicity upon injections into patients.

Protein aggregation in solution occurs as a result of attractive protein-protein interactions (PPI). PPI can be characterized using techniques such as dynamic light scattering which measures the dependence of molecular diffusivity on ...


Development And Characterization Of Improved Red Fluorescent Protein Variants, Premashis Manna Jan 2018

Development And Characterization Of Improved Red Fluorescent Protein Variants, Premashis Manna

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

Aequorea victoria-based green fluorescent proteins and their blue, cyan and red counterparts offer unprecedented advantage as biological markers owing to their genetic encodability and straightforward expression in different organisms. Fluorescent proteins are characterized with complex photo-kinetics due to the presence of light-induced non-fluorescent or dark states which are responsible for their fluorescence intermittency or 'blinking'. We developed time- and frequency-domain techniques for probing the kinetics involving dark state conversion (DSC) and ground state recovery (GSR) in red fluorescent proteins (RFPs). Ensemble-level DSC and GSR kinetics in FPs were presented in the context of their single molecule fluorescence behaviors. Although ...


In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski Jan 2018

In Situ Designer Lipid Production: Integration Of Novel Characteristics And Behaviors Into Synthetic Cell Membranes, Danielle S. Konetski

Chemical & Biological Engineering Graduate Theses & Dissertations

This thesis investigated the coupling of lysolipids and functionalized tails for in situ formation of synthetic liposomes designed to enable specific characteristics or behaviors in applications ranging from drug delivery to the advancement of artificial cell development. Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) mediated lipid coupling was improved via incorporation of a photoinitiation system. Here, photo-CuAAC enabled spatiotemporal control over liposome assembly, an over 400-fold increase in formation density, and control over the maximal cross-sectional area of the liposomes formed.

Thiol-Michael mediated lipid coupling was enabled using thiol-functionalized lysolipids and acrylate tails where phospholipids were produced over 48 hours with approximate 90 ...


Biomimetic Hydrogels To Support Bone Development For Osteochondral Tissue Engineering Applications, Aaron H. Aziz Jan 2018

Biomimetic Hydrogels To Support Bone Development For Osteochondral Tissue Engineering Applications, Aaron H. Aziz

Chemical & Biological Engineering Graduate Theses & Dissertations

Osteochondral tissue engineering using multilayer hydrogel scaffolds to recapitulate the native layered structure serves as a promising strategy to repair and regenerate osteochondral defects due to joint trauma, injuries, and long-term diseases. The gold standard of clinical therapies relies on replacing the defect with autologous tissue from a non load-bearing site, yet these techniques often fail due to a lack of mechanical anchoring, donor site morbidity and tissue availability. Multilayered photopolymerizable poly(ethylene glycol) (PEG) hydrogels, with a bony layer that facilitates osteoconductivity and osseointegration present an opportunity to overcome these issues. Ensuring multilayer hydrogels supported bone growth within the ...


Modeling And Designing Genetic Devices Using Transcriptional Interference In Escherichia Coli, Antoni Escalas Bordoy Jan 2018

Modeling And Designing Genetic Devices Using Transcriptional Interference In Escherichia Coli, Antoni Escalas Bordoy

Chemical & Biological Engineering Graduate Theses & Dissertations

Microorganisms inhabit every extreme location of our planet. In their journey through the ages, they have been able to incredibly adapt to a myriad of different environmental conditions. A key mechanism for their success has been their ability to respond to environmental and nutritional changes through regulatory programs primarily encoded at the transcriptional level. This adaptability to new environments is what encourages scientists to believe in engineering a biological revolution that will transform our lives due to its potential to result in innovative approaches for bioremediation, sustainable energy production, and biomedical therapies. This thesis explores the potential of the phenomenon ...


Modifying Supported Palladium Catalysts Using Self-Assembled Monolayers For Liquid-Phase Reactions, Pengxiao Hao Jan 2018

Modifying Supported Palladium Catalysts Using Self-Assembled Monolayers For Liquid-Phase Reactions, Pengxiao Hao

Chemical & Biological Engineering Graduate Theses & Dissertations

In this thesis, we investigate the modification of Pd/Al2O3 catalyst using thiolate and phosphonate self-assembled monolayers (SAMs) for two types of reactions in liquid-phase environments: selective oxidation of α, β-unsaturated alcohols and vanillin hydrodeoxygenation. Through the study, we aim to gain a better understanding of the liquid-solid interface that governs the catalytic activity.

The selective oxidation of a linear unsaturated alcohol trans-2-hexen-1-ol (HOL) was studied on Pd/Al2O3 catalysts in heptane. Alkanethiolate self-assembled monolayers (SAMs) were applied to modify Pd/Al2O3 catalysts; the sulfur content on the Pd surface was ...


Application Of Advances In Synthetic Biology To Modular Megasynthase Design And Scalable Combinatorial Biosynthesis, William Clifford Grau Jan 2018

Application Of Advances In Synthetic Biology To Modular Megasynthase Design And Scalable Combinatorial Biosynthesis, William Clifford Grau

Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)

The diversity in structure and biological activity of natural products has long captured the imagination of chemists and molecular biologists alike. Natural products are synthesized by elaborate secondary metabolic pathways or enzymes. For example, modular megasynthases such as Type I Polyketide Synthases (PKSs), Non-ribosomal Peptide Synthetases (NRPSs), and PKS-NRPS hybrids, produce an array of quintessential natural products. The apparent modularity, defined architecture, and predictable chemistry of modular megasynthases make them especially attractive for combinatorial biosynthesis, which has long been a focus of biotechnology. Combinatorial biosynthetic libraries are of particular interest due to the high value of many natural products and ...


Connecting Protein Structure And Dynamics On Biomaterials With The Foreign Body Response, David Faulon Marruecos Jan 2018

Connecting Protein Structure And Dynamics On Biomaterials With The Foreign Body Response, David Faulon Marruecos

Chemical & Biological Engineering Graduate Theses & Dissertations

The harsh environment of the foreign body response (FBR) has the potential to negatively impact the implantations of biomaterials in the body. The FBR is initiated by inflammatory cells that recognize the material as foreign through surface-adsorbed proteins. When proteins interact with surfaces, they can unfold and expose epitopes that may be recognized by immune cells and trigger a series of reactions. Importantly, the presentation of unfolded proteins is directly influenced by the highly dynamic and heterogeneous behavior of proteins in near-surface environments, as well as by the physicochemical features of the underlying surface. Such behavior is the result of ...


Multicatalytic, Light-Driven Reactions For Improved Biofuels And Wastewater Remediation, Glenn Richard Hafenstine Jan 2018

Multicatalytic, Light-Driven Reactions For Improved Biofuels And Wastewater Remediation, Glenn Richard Hafenstine

Chemical & Biological Engineering Graduate Theses & Dissertations

Utilizing solar driven photocatalysis to facilitate coupling reaction systems is a promising route for low-energy and selective production of valuable chemicals. My doctoral research has focused on using photocatalytic cadmium sulfide nanorods to run novel reaction systems under mild, ambient conditions with sunlight as the only energy source. In this dissertation, I will first describe a cascade reaction system for catalytic upgrading of the fermentation products butanol and ethanol into an easily separated product in 2-ethylhexenal with a higher energy density. Specifically, I will report the use of the enzyme alcohol dehydrogenase, regeneration of the enzymatic cofactor by the photocatalyst ...


Engineering The Phospholipid Monolayer On Fluorocarbon, Hydrocarbon, And Liquid Crystal Nanodroplets For Applications In Biosensing, Rajarshi Chattaraj Jan 2018

Engineering The Phospholipid Monolayer On Fluorocarbon, Hydrocarbon, And Liquid Crystal Nanodroplets For Applications In Biosensing, Rajarshi Chattaraj

Mechanical Engineering Graduate Theses & Dissertations

Nanodroplets (NDs) are liquid-in-liquid dispersions of ~100-800 nm size range that are often stabilized by a shell of lipids, polymer, proteins, or surfactants. NDs have been explored for a variety of biomedical applications, mostly involving drug formulation and delivery. However, the unique properties of encapsulated liquids, and the effects of interfacial chemistry on these properties, makes NDs potentially powerful candidates for new biosensing technologies. This dissertation explores different oil-in-water or fluorocarbon-in-water ND systems for in-solution sensing of biomarkers as both a platform for diagnostic assays and as a precursor to in vivo biosensing. Nanodroplets, because of their size, provide a ...


The Coordination Of Movement From Motor Units To Muscle Synergies, Daniel Francis Feeney Jan 2018

The Coordination Of Movement From Motor Units To Muscle Synergies, Daniel Francis Feeney

Integrative Physiology Graduate Theses & Dissertations

This dissertation comprises computational and experimental studies that examined the neuromuscular factors underlying differences in manual dexterity and mobility in health and disease.

The first two studies developed models of motor unit force production. The first model used a Proportional-Integral-Derivative (PID) control algorithm to activate a pool of motor units to simulate the force trajectory during force-matching tasks. The second model comprised a probabilistic state-space model to estimate the common synaptic input to motor neurons based on the discharge times of action potentials by activated motor units. The state-space model demonstrated superior sensitivity compared with previous models.

The next three ...


Crispr-Assisted Interrogation And Engineering Of Metabolic Pathways, Marcelo Colika Bassalo Jan 2018

Crispr-Assisted Interrogation And Engineering Of Metabolic Pathways, Marcelo Colika Bassalo

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Since Mendel’s work established the basis of inheritance in the late 1800s, multiple decades of research characterized monogenic traits across all domains of life. Yet, we still have a fairly limited knowledge on the genotype behind the vast majority of phenotypes. It is now evident that discrete biological functions can rarely be linked to a single gene. Further, these multigenic traits are often interconnected via a sophisticated and robust metabolic and regulatory network, selected by evolution in order to optimally distribute resources. The complexity of these multigenic traits challenges traditional genetic tools, broadly limiting our capability to understand and ...


Skeletal Muscle Metabolism: From Tissue To Stem Cell, Katherine Elise Gadek Jan 2018

Skeletal Muscle Metabolism: From Tissue To Stem Cell, Katherine Elise Gadek

Molecular, Cellular, and Developmental Biology Graduate Theses & Dissertations

Excessive circulating triglycerides due to reduction or loss of lipoprotein lipase (LPL) activity contribute to hypertriglyceridemia and increased risk for pancreatitis. The only gene therapy treatment for LPL deficiency decreases pancreatitis but minimally reduces hypertriglyceridemia. Synthesized in striated muscle and adipose tissue, LPL is then trafficked to blood vessel endothelial cells where it hydrolyzes triglycerides into free fatty acids. We conditionally knocked out LPL in differentiated striated muscle tissue lowering striated muscle LPL activity and causing hypertriglyceridemia. We crossed these LPL knockout mice with mice possessing a conditional avian retroviral receptor gene and infected mice with human LPL or mCherry ...


Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi Jan 2018

Modeling Hypertrophic Cardiomyopathy In Neonatal Cardiomyocytes In Cultured In 3d, Esther Choi

Undergraduate Honors Theses

Heart disease continues to be the leading cause of death in both men and women. It is accompanied with cellular- and organ- level remodeling that cannot be mimicked or recaptured by cells on standard culture platforms. Polyethylene glycol hydrogels bridge this gap by providing user-defined tunability for cell culture. A novel 3D cell culture system was developed to encapsulate neonatal rat ventricular cardiomyocytes (NRVMs) in a single plane, and cell health was assessed. In parallel, recombinant adenoviruses containing the mutations responsible for human hypertrophic cardiomyopathy (HCM) in the beta myosin gene (R403Q and R453C) were generated and used as a ...


Characterization Of The Properties Of Photopatterned Hydrogels For Use In Regenerative Medicine, Callie Irene Fiedler Apr 2017

Characterization Of The Properties Of Photopatterned Hydrogels For Use In Regenerative Medicine, Callie Irene Fiedler

Electrical, Computer & Energy Engineering Graduate Theses & Dissertations

The goal of this thesis was to locally photopattern cytocompatible hydrogels to exhibit a wide range of mechanical properties and to probe the fundamental parameters governing these materials printed via stereolithography (SLA). Fabricating cell-laden structures with locally defined mechanical properties is non-trivial because the use of multiple precursor materials is wasteful, slow, and can lead to cell-death. To investigate the range of mechanical properties a single precursor solution can produce, I initially formed a single-network hydrogel and cyclically in- swelled fresh precursor solution followed by photo-exposure of the swollen gel (“swelling + exposure” or SE cycle). Because transport (i.e., diffusion ...


Radiometric Thermometry For Wearable Deep Tissue Monitoring, Parisa Momenroodaki Apr 2017

Radiometric Thermometry For Wearable Deep Tissue Monitoring, Parisa Momenroodaki

Electrical, Computer & Energy Engineering Graduate Theses & Dissertations

Microwave thermometry is an attractive non-invasive method for measuring internal body temperature. This approach has the potential of enabling a wearable device that can continuously monitor core body temperature. There are a number of health-related applications in both diagnostics and therapy, which can benefit from the knowledge of core body temperature. However,there are a limited number of device solutions, which are usually not wearable or cannot continuously monitor internal body temperature non-invasively. In this thesis, a possible path toward implementing such a thermometer is presented. The device operates in the "quiet" frequency band of 1.4 GHz which is ...


The Role Of Physiochemical Cues On Msc Differentiation And Tissue Regeneration In A Cartilage Biomimetic Hydrogel, Elizabeth Anne Aisenbrey Jan 2017

The Role Of Physiochemical Cues On Msc Differentiation And Tissue Regeneration In A Cartilage Biomimetic Hydrogel, Elizabeth Anne Aisenbrey

Chemical & Biological Engineering Graduate Theses & Dissertations

Cartilage defects, whether caused by injury or disease, often lead to further degeneration and ultimately osteoarthritis. Current treatments such as autologous chondrocyte implantation (ACI) and microfracture have shown limited success at repairing and regenerating cartilage tissue, thus an alternative treatment is necessary. Cartilage tissue engineering using scaffolds as a vehicle to deliver cells, biochemical cues, and soluble factors presents a promising strategy to regenerate cartilage tissue. Photopolymerizable poly(ethylene glycol) (PEG) hydrogels that can be modified with different biochemical moieties, degradable and nondegradable peptides, and therapeutic agents are an attractive platform to design and develop scaffolds that can be tailored ...


Zonal Articular Cartilage Possesses Complex Mechanical Behavior Spanning Multiple Length Scales: Dependence On Chemical Heterogeneity, Anisotropy, And Microstructure, Joseph Anders Wahlquist Jan 2017

Zonal Articular Cartilage Possesses Complex Mechanical Behavior Spanning Multiple Length Scales: Dependence On Chemical Heterogeneity, Anisotropy, And Microstructure, Joseph Anders Wahlquist

Mechanical Engineering Graduate Theses & Dissertations

This work focused on characterizing the mechanical behavior of biological material in physiologically relevant conditions and at sub millimeter length scales. Elucidating the time, length scale, and directionally dependent mechanical behavior of cartilage and other biological materials is critical to adequately recapitulate native mechanosensory cues for cells, create computational models that mimic native tissue behavior, and assess disease progression. This work focused on three broad aspects of characterizing the mechanical behavior of articular cartilage. First, we sought to reveal the causes of time-dependent deformation and variation of mechanical properties with distance from the articular surface. Second, we investigated size dependence ...


Evaluation Of Fibrous Polymeric Coating Over Vascular Stent Material, Parnaz Boodagh Jan 2017

Evaluation Of Fibrous Polymeric Coating Over Vascular Stent Material, Parnaz Boodagh

Civil Engineering Graduate Theses & Dissertations

The field of percutaneous coronary intervention has seen a plethora of advances over the past few decades, which have allowed for its development into safe and effective treatments for patients suffering from cardiovascular diseases. However, in-stent thrombosis and restenosis remain clinically significant problems.

This dissertation proposes a methodology to potentially overcome in-stent thrombosis and restenosis by designing and fabricating a polymeric coating with mechanical and surface properties inert to cell adhesion and platelet attachment. First, conventional electrospinning technique was used to fabricate polyethylene glycol dimethacrylate/ poly l-lactide acid (PEGDMA/PLLA) blend fiber substrate with different composition ratios. Next, coaxial electrospinning ...


Zonal Articular Cartilage Possesses Complex Mechanical Behavior Spanning Multiple Length Scales: Dependence On Chemical Heterogeneity, Anisotropy, And Microstructure, Joseph Anders Wahlquist Jan 2017

Zonal Articular Cartilage Possesses Complex Mechanical Behavior Spanning Multiple Length Scales: Dependence On Chemical Heterogeneity, Anisotropy, And Microstructure, Joseph Anders Wahlquist

Mechanical Engineering Graduate Theses & Dissertations

This work focused on characterizing the mechanical behavior of biological material in physiologically relevant conditions and at sub millimeter length scales. Elucidating the time, length scale, and directionally dependent mechanical behavior of cartilage and other biological materials is critical to adequately recapitulate native mechanosensory cues for cells, create computational models that mimic native tissue behavior, and assess disease progression. This work focused on three broad aspects of characterizing the mechanical behavior of articular cartilage. First, we sought to reveal the causes of time-dependent deformation and variation of mechanical properties with distance from the articular surface. Second, we investigated size dependence ...


Understanding The Mechanics Of Tissue Growth In Engineered Scaffolds: Case Of Cartilage Tissue, Umut Akalp Jan 2017

Understanding The Mechanics Of Tissue Growth In Engineered Scaffolds: Case Of Cartilage Tissue, Umut Akalp

Civil Engineering Graduate Theses & Dissertations

Tissue failure due to aging or diseases reduces the quality of life for individuals. In the case of cartilage tissue, the current solution is to use implants to fulfill the functional duties of native tissue. However, this approach has limitations, such as periodic replacement and number of the required operations. Tissue engineering provides an alternative approach in which the aim is to regenerate the native tissue by a population of cells encapsulated in a scaffold (i.e. hydrogels). Although this approach is promising, there are several limitations regarding the design of these scaffolds which can be overcome only by the ...