Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Biomedical Engineering and Bioengineering
Isolation And Production Of Tandem Collagen Binding Domain From Clostridial Collagenase Colg And Developments In C1q Reagent Production For Future Molecule Characterization Work, Stephanie Beitle
Chemical Engineering Undergraduate Honors Theses
This thesis covers a two part project: the production methods to create a double collagen binding domain molecule with a growth factor for wound healing applications and the development of a new in-house production method for isolating C1q from bovine blood. The wound healing molecule was created using transformation, sonication, and purification before being tested via electrophoresis SDS page and Western blots to confirm the molecule’s presence. The C1q in-house production method utilizes an ultrafiltration flow cell rather than dialysis at a critical point in the process, allowing for researchers to not only be able to use a single small …
Monitoring Fgf1-Treated Skin Wounds With Label-Free Multiphoton Microscopy, Gianna Busch
Monitoring Fgf1-Treated Skin Wounds With Label-Free Multiphoton Microscopy, Gianna Busch
Biomedical Engineering Undergraduate Honors Theses
Chronic skin wounds pose a significant threat to public health, affecting as many as 5.5 million people in the United States every year and costing the healthcare system $10 billion annually1,2. These wounds are associated with prolonged inflammation, poor vascularization, increased infection risk, and high mortality rates3,4. There is significant interest in developing and testing a variety of biologics to promote wound healing. However, quantitative evaluations of healing are typically limited to measurements of wound size, and there is a critical need to develop quantitative biomarkers sensitive to different aspects of the healing process. Label-free multiphoton …
Guide Rnas Preparation For In-Vitro Crispr-Cas9 Complex Delivery Targeting Genes That Affect Wound Healing., Prashant Khatiwada
Guide Rnas Preparation For In-Vitro Crispr-Cas9 Complex Delivery Targeting Genes That Affect Wound Healing., Prashant Khatiwada
Biomedical Engineering Undergraduate Honors Theses
CRISPR-Cas9 technology has widely been used as a viable genome engineering platform to make site-specific insertion, deletion, and breaks. The nuclease dead version of Cas9 or dCas9 can be used for the activation and repression of target gene sites using specific activation or repression domains. In this study, CRISPR guide RNAs were designed for a CRISPR inhibition approach to repress the transcriptional activity of the target genes. An expression plasmid vector composed of a U6 promoter sequence, BbsI restriction sites, and a chimeric gRNA sequence was digested, and the phosphorylated forward and reverse gRNAs were ligated with the plasmid vector. …
Multiphoton Microscopy And Deep Learning Neural Networks For The Automated Quantification Of In Vivo, Label-Free Optical Biomarkers Of Skin Wound Healing, Jake D. Jones
Graduate Theses and Dissertations
Non-healing ulcerative wounds that occur frequently in diseases such as diabetes are challenging to diagnose and treat due to numerous possible etiologies and the variable efficacy of wound care products. With advanced age, skin wound healing is often delayed, leaving elderly patients at high risk for developing these chronic injuries. As it is challenging to discriminate age-related delays from disease-related chronicity, there is a critical need to develop new quantitative biomarkers that are sensitive to wound status. Multiphoton microscopy (MPM) techniques are well-suited for 3D imaging of epithelia and are capable of non-invasively detecting metabolic cofactors (NADH and FAD) without …