Biomedical Engineering and Bioengineering Commons^™

Homocysteine Reduction For Stroke Prevention: Regarding The Recent Aha/Asa 2021 Prevention Of Stroke In Patients With Stroke And Transient Ischemic Attack, Craig Brown, Jianhua Wang, Hong Jiang, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

Reduction of secondary ischemic stroke risk following an initial stroke is an important goal. The 2021 Prevention of Stroke in Patients With Stroke and Transient Ischemic Attack assembles opportunities for up to 80% secondary stroke reduction. Homocysteine reduction was not included in the recommendations. The reduction of homocysteine with low doses of folic acid has been shown to reduce ischemic stroke and all stroke. This has been obscured by studies using high doses of folic acid and cyanocobalamin in patients with renal failure and Methylenetetrahydrofolate reductase (MTHFR) polymorphisms. The confounding impacts of high dose folic acid and cyanocobalamin toxicity in …

S8e10: How Can Nature-Inspired Engineering Improve Human Health?, Ron Lisnet, Caitlin Howell

The Maine Question

Antibiotic resistance has become a growing problem in the treatment of bacterial infections. In addition to minimizing or negating the effects of existing medicine, these antibiotic-resistant bacteria, or “superbugs,” are mutating faster than the development of new remedies.

Caitlin Howell, University of Maine associate professor of biomedical engineering, is working on new tools that take notes from nature to combat antibiotic-resistant bacteria. Similar to the way in which the human body keeps balance with its own bacterial populations, Howell’s devices use nontoxic, non-invasive surface-based technology to trap bacteria and prevent them from spreading.

In this episode of “The Maine Question” …

Medical Foods For Lowering Homocysteine In Hypertensive Patients, Merrill F. Elias, Craig J. Brown

Maine-Syracuse Longitudinal Papers

In a recent commentary, Elias and Brown review the history of skepticism about the value of homocysteine for preventing hypertension and stroke. They argue that this skepticism is not supported by recent literature. This paper was stimulated by a recent comprehensive review of the literature on this topic by Smith and Refsum. Prior to these papers, a commentary and review by Skeete and DiPette provided evidence for the value of homocysteine-lowering in the treatment of hypertension. Elias and Brown supported this conclusion. Both papers emphasized that more work is needed to clarify the beneficial effects of lowering homocysteine.

Elias and …

Wavelet-Based Automatic Breast Segmentation For Mammograms, Basel White

Honors College

As part of a first of its kind analysis of longitudinal mammograms, there are thousands of mammograms that need to be analyzed computationally. As a pre- processing step, each mammogram needs to be converted into a binary (black or white) spatial representation in order to delineate breast tissue from the pectoral muscle and image background, which is called a mammographic mask. The current methodology for completing this task is for a lab member to manually trace the outline of the breast, which takes approximately three minutes per mammogram. Thus, reducing the time cost and human subjectivity when completing this task …

New Evidence For Homocysteine Lowering For Management Of Treatment-Resistant Hypertension, Merrill F. Elias, Craig J. Brown

Maine-Syracuse Longitudinal Papers

Treatment-resistant hypertension is a topic of great importance and the subject of editorial comments in the American Journal of Hypertension. Homocysteine is an intermediate compound in the vitamin regulated One Carbon Methylation pathway which affects glutathione, methionine, and nitric oxide metabolism. Elevated homocysteine, triggered by genetic mutations or insufficient body stores of active vitamins B6, B12, folate, riboflavin, indicates oxidative stress and is associated with impaired nitric oxide synthesis causing small vessel vasoconstriction in the central nervous system. High homocysteine (defined later) is also a risk factor for hypertension.

Higher Yogurt Intake Is Associated With Lower Blood Pressure In Hypertensive Individuals: Cross-Sectional Findings From The Maine–Syracuse Longitudinal Study, Alexandra T. Wade, Benjamin A. Guenther, Fayeza S. Ahmed, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

Associations between fermented dairy products and blood pressure are unclear. The current study therefore examined the association between yogurt and blood pressure in hypertensive and non-hypertensive individuals. Cross-sectional analyses were undertaken on 915 community-dwelling adults from the Maine-Syracuse Longitudinal Study. Habitual yogurt consumption was measured using a food frequency questionnaire. The primary outcomes were systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), and pulse pressure. Secondary outcomes included BMI (kg m^-2), total cholesterol (mg dL^-1), glucose (mg dL^-1), HDL (mg dL^-1), LDL (mg dL^-1), triglycerides (mg …

Reclaiming The Importance Of Homocysteine As A Marker Of Cardiovascular And Neurologic Disease, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

The Smith and Refsum review of ‘Homocysteine’ – from biomarker to disease prevention in this issue of the Journal of Internal Medicine [1 – is much more than an updated review of the literature. It provides a history of the progression of studies on homocysteine in relation to cardiovascular disease and neurological disorders from its earliest days to the present time. It is a scholarly examination of the mounting evidence of the importance of homocysteine as a biomarker for cardiovascular and neurologic disease and the utility of B vitamins and folate as a treatment.

The Hate Within, Kora Kukk

Honors College

A novel in its beginning stages, The Hate Within (May 2021) is the product of discovering a way to be able to eloquently combine biomedical engineering with creative writing which will intrigue young adult readers. This thesis contains the first few chapters of the story along with an additional journal entry, the story’s outline with a list of plot events, and a disquisition exploring the motivation and methodology behind creating this thesis.The novel is about a junior in high school named Alaita. She is a super sweet, kind, and popular girl in school until one day she is diagnosed with …

Scientific Knowledge Shaping Perceptions Of Bioengineered Food Among Undergraduate Students, Kathleen Tims

Honors College

While commonplace in our grocery stores, GMO-foods have persevered through years of controversy. As the availability of bioengineered(BE) commodities grows and biotechnical industries make strides, studies have shown that the average consumers’ knowledge concerning biotechnology has not. In addition to widely held misconceptions and the general lack of knowledge regarding bioengineered commodities reported by young adults, especially students, the recent United States Department of Agriculture mandate for BE product labeling prompts further research into the consumer perspective on bioengineered products. This research looked to expand upon prior work regarding students’ individual perspective towards topics such as GMOs, knowledge of BE …

A Method For Orientation Of Cellulose Nano Fibers For Addition Of Biological Nanoparticles And Tissue Integration, Joshua Hamilton

Honors College

The University of Maine is one of the world’s leading producers of cellulose nanofibers (CNF). This material has the power to revitalize the Maine paper industry. This has led to an abundance of labs on campus researching applications for the material. Active research activities at the University of Maine include industrial applications such as filtration, biodegradable packaging, building materials and niche health care applications. Niche health care applications include wicking pads for point of care diagnostics and tunable biomaterials ranging from dressings to implantable bone-like materials. Medical applications require an analysis of the biocompatibility of CNF.CNF is biocompatible in general: …

Conductive Agnw/Tempo Cnf Thin Film, David Flewelling

Honors College

Cellulose is a strong, readily available biologically sourced polymer with a wide potential for applications in many fields. Its high degree of biodegradability makes it an excellent candidate for environmentally friendly sensors, but it is necessary to devise a method by which to render it conductive. Silver nanowires are highly conductive nanoparticles with many potential applications in sensing, as well as being on a similar order-of-magnitude in size as cellulose derivatives, which makes them a prime candidate for use in this application. Methods of casting cellulose nanofibrils (CNF), cellulose nanocrystals (CNC), and TEMPO-Oxidized CNF (TEMPO CNF) films were explored, and …

Computational Analysis And Classification Of Shg Images Of Cancerous Pancreatic Tissue Based On Collagen Fiber Alignment, Gerren Welch

Honors College

Pancreatic cancer is a deadly disease, with a low five-year survival rate partly due to the difficulty in diagnosing the cancer early in its development, as it shares symptoms with more common and less lethal conditions. Using Second Harmonic Generation (SHG) microscopy and computer analysis, our knowledge of the biophysics of the pancreatic tumor microenvironment increases which may lead to the development of more effective therapies. In collaboration with Maine Medical Center Research Institute (MMCRI), we have identified 20 pancreatic cancer patients. In these 20 pancreatic cancer patients, Dr. Jones, a pathologist at MMCRI has identified normal adjacent pancreas, fibrotic …

Black Bear To Merin Skincare: Alumni Founders Adding Value To Maine's Lobster Industry, Ashley Forbes

General University of Maine Publications

It started in 2013 when co-founders Amber Boutiette and Patrick Breeding met on the first day of their first year as biomedical engineering undergraduate students at the University of Maine. Nearly eight years later the pair relocated to Portland, launched Marin Skincare--a specialty skincare product made with lobster glycoprotein--and are now teaming with an established Maine brand, Luke's Lobster, to scale their supply chain and meet strong demand for their first product.

The Perils Of Automated Wrist-Cuff Devices And Dental Chairs In Opportunistic Blood Pressure Screening, Merrill F. Elias, Amanda L. Goodell, Adam Davey

Maine-Syracuse Longitudinal Papers

In our opinion, the automated arm-cuff method should be used where the goal is OBPS and the patient should be properly seated at a table with BP measurement conducted following the recommendations of the American Heart Association.

Human Errors In Automated Office Blood Pressure Measurement, Merrill F. Elias, Amanda L. Goodell

Maine-Syracuse Longitudinal Papers

In this review of the literature and commentary, we examine the literature on automated blood pressure (BP) measurements in the office and clinic. Our purpose is to revisit issues as to the pros and cons of automated BP measurement published in Hypertension in June 2020 and to identify areas needing additional research. Despite initial reservations about automated BP, it is here to stay. A number of experts suggest that human error will be reduced when we move from the more complex skills required by aneroid sphygmomanometer measurement to the fewer skills and steps required by automated BP measurement. Our review …

Leisure Activity For Dementia Prevention. More Work To Be Done., Victor W. Henderson, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

Dementia prevention is a worldwide priority because of the aging population, devastating consequences for patients and families, and the drain on societal resources. In the absence of disease-modifying therapies, there is interest in lifestyle factors that might prevent or delay the onset of dementia. Chief among these is leisure activity, conceptualized as pursuits undertaken for relaxation or pleasure after completion of essential chores and occupational responsibilities. Leisure activity invokes the cornerstones of cognitive reserve: mental activity, physical activity, and social engagement.

Exploration Of The Relationship Between The Fractal Dimension Of Microcalcification Clusters And The Hurst Exponent Of Background Tissue Disruption In Mammograms, Betelhem Abay

Honors College

Breast cancer is one of the most frequent cancers among women worldwide and holds the second place in cancer-related death. Mammography is the most commonly used screening technique, however, the dense nature of some breasts makes the analysis of mammograms challenging for radiologists. The 2D Wavelet Transform Modulus Maxima (WTMM) is one mathematical approach that is used to for the analysis of mammograms. In 2014, a team from the CompuMAINE Lab characterized differences between benign microcalcification clusters (MC) from malignant MC by calculating their fractal dimension, D, with the aid of the 2D WTMM method. In a different implementation of …

Comparison Of Drying Methods Of Cellulose Nanofibrils Low Density Foams And Their Effects On Structural Properties, Lauren Ryan

Honors College

Cellulose nanofibrils (CNF) are a promising biomaterial made of cellulose, the most abundant biopolymer on the planet, and are produced in mass quantities by the Process Development Center at the University of Maine.One promising application of these materials are low-density foams, or aerogels, which are commonly prepared using lyophilization and solvent-exchange methods. Each of these methods rely on different physical and chemical processes to remove liquid from a given substrate and may impact the overall structure of a material in different ways. The purpose of this work was to determine if these drying techniques have any significant effect on the …

Adherence To A Mediterranean Diet Associated With Lower Blood Pressure In A Us Sample: Findings From The Maine- Syracuse Longitudinal Study, Fayeza S. Ahmed, Alexandra T. Wade, Benjamin A. Guenther, Karen J. Murphy, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

Hypertension is a key modifiable risk factor for cardiovascular disease. The Mediterranean diet (MedDiet) may be associated with improvements in blood pressure. However, few studies have examined the association between MedDiet adherence and blood pressure in non-Mediterranean populations, and findings are mixed. We analyzed cross-sectional data (Wave 6) for 851 participants of the Maine-Syracuse Longitudinal Study. MedDiet adherence was calculated using food frequency questionnaire data and a literature-based MedDiet adherence score. Dependent variables included systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse pressure (PP), and mean arterial pressure (MAP). Separate linear robust regression analyses revealed significant associations between MedDiet …

Adherence To A Mediterranean Diet Is Associated With Cognitive Function In An Older Non- Mediterranean Sample: Findings From The Maine- Syracuse Longitudinal Study, Alexandra T. Wade, Merril F. Elias, Karen J. Murphy

Maine-Syracuse Longitudinal Papers

Background: Adherence to a Mediterranean diet is associated with higher cognitive function and reduced risk of dementia in Mediterranean populations. However, few studies have investigated the association between Mediterranean diet adherence and cognition in populations outside of the Mediterranean basin. Furthermore, it is currently unknown whether the association between Mediterranean diet adherence and cognitive function differs between middle-aged and older individuals. Methods: Cross-sectional (n = 894) and longitudinal (n = 530) multivariable analyses were undertaken using data from community-dwelling adults from the Maine-Syracuse Longitudinal Study (MSLS). Mediterranean diet adherence was measured by applying a literature-based Mediterranean diet score to food …

Setting The Record Straight For Two Heroes In Hypertension: John J. Hay And Paul Dudley White, Merrill Elias, Amanda L. Goodell

Maine-Syracuse Longitudinal Papers

Papers dealing with the history of the study of hypertension provide an invaluable window to our past. They identify our misconceptions, mistakes, and advances and describe the evolution of new knowledge. New knowledge develops in the context of contemporary scientific knowledge and zeitgeist. Zeitgeist is the German word for the defining mood or spirit of a particular era as expressed in the ideas and beliefs of the time.

The limitations of a scientist's knowledge and perspective at any given point in time must be considered in historical context. Therefore, we must tread carefully when criticizing a scientist's thinking and ideas …

Arsenic Remediation Of Maine Drinking Water, Austin Steward

Honors College

Arsenic, a metalloid, is one of the most prominent toxins in Maine drinking water. There are approximately 86,500 Maine citizens exposed to water containing arsenic over the maximum contamination level causing adverse effects including nausea, multiple cancers, and a reduction of full scale IQ and executive function. In drinking water, arsenic arises both by the natural leaching from bedrock and from the use of chemicals such as pesticides, embalming fluids, and wood preservatives. Although there are many known arsenic water remediation techniques, finding a method compatible for multiple arsenic isotopes is challenging. In this work, we test the low-energy and …

Design Of The Maine-Syracuse Longitudinal Study, Merrill F. Elias

Maine-Syracuse Longitudinal Papers

The Maine-Syracuse Longitudinal Study (MSLS) uses a design that was very popular in life-span aging research in 1960 and for many years after. Starting with baseline the original cohort is followed over many years. Outcome measures are obtained at distinct time intervals, but new cohorts of individuals join the study at each specific period.

Implantation Of Nanocellulose In The Zebrafish Model, Hanna J. Anderson

Honors College

The number of novel materials for use in biomedical implantation is expanding rapidly, increasing the success rates of implant procedures. Nanocellulose is being assessed as a sustainable and biocompatible material, offering an alternative to conventional polymer or metal designs with the appropriate structure for potential tissue integration. In this research, the capacity of cellulose nanofibers to serve as biomedical implants is assessed through examination of immune responses of transgenic zebrafish, utilizing bright field and confocal fluorescence microscopy. Methods for creating microincisions for the implantation of dense cellulose nanofiber shards in the zebrafish model were explored, and a surgical protocol was …

Systolic Orthostatic Hypotension Is Related To Lowered Cognitive Function: Findings From The Maine-Syracuse Longitudinal Study, Rachael V. Torres, Merrill Elias, Georgina E. Crichton, Gregory A. Dore, Adam Davey

Maine-Syracuse Longitudinal Papers

The aim of the present study was to examine the relationship between orthostatic changes in blood pressure (BP) and cognition, with consideration given to cardiovascular risk factors and lifestyle variables. The cross-sectional analysis included 961 community-dwelling participants of the Maine-Syracuse Longitudinal Study, for whom BP clinic measures (five sitting, five recumbent, and five standing) were obtained. Eighteen percent of participants had orthostatic hypotension (fall in systolic BP ≥20 mm Hg or diastolic BP ≥10 mm Hg upon standing) and 6% had orthostatic hypertension (rise in systolic BP ≥20 mm Hg). Orthostatic hypotension and hypertension defined using traditional criteria were unrelated …

Stabilization Of Iron Oxide Nanoparticles For Homogenous Integration Into Cellulose Nano Fibrils, Banton H. Heithoff

Honors College

Cellulose nanofibrils is one of the future potential giants in the medical implant industry. Its unique properties make it the ideal material for use in both permanent prosthetic devices and non- permanent implants such as screws and plates. To increase the usability of this material, the addition of super paramagnetic iron oxide nanoparticles is needed to gain MRI and X-Ray visibility. The methodology for how to homogeneously integrate these particles into the system using the addition of coating agents is explored. This research demonstrates that the addition of coating agents to the iron oxide nanoparticles can affect both the pH …

Host Range Of Mycobacteriophage And Exploring Lysogenic Relationships Between Mycobacteriophage And Their Hosts, Emily Illingworth

Honors College

Mycobacteriophage (phage) are abundant viruses that infect bacteria of the genus Mycobacterium. Pathogenic species of Mycobacterium carry prophage that are hypothesized to contribute to virulence. The term “prophage” describes a dormant phage during lysogeny, which occurs when a phage genome integrates itself into the host genome. Understanding the interactions between bacterial host and prophage may improve our ability to treat disease caused by bacteria. Phage are highly diverse and are sorted into clusters based on genome sequence similarity. To determine which phage infect and form lysogens in mycobacterial fish pathogens, we applied viral dosages of phage representative of clusters A–X …

Citrate’S Counter Ions And Ph Effect On Gold Nanoparticle Growth Kinetics, Andrew B. Closson

Honors College

The effects of citrate’s counterions and pH are examined in gold nanoparticle growth kinetics. The growth of gold nanoparticles from citrate reduction is a common method used for creating particles from 10-120 nm with a well-defined size distribution. TEM observation of the seed-mediated growth shows an intermediate aggregation phase that has been shown by researchers to exist in other solution-phase nanocrystal growth. We have shown that this intermediate agglomeration phase within particle growth may be avoided with certain pairings of counterion and pH. This aggregation phase has no noticeable effects on the formation of the particles at a smaller scale …

The Role Of Ship1 In The Innate Immune System During An Influenza A Viral Infection, Spencer Earle Traxler

Honors College

The SHIP1 gene is a member of the inositol polyphosphate-5-phosphate (INPP5) and its expressed protein functions as a negative regulator of myeloid cell proliferation, survival, and migration. Mutations in this gene are associated with various defects and cancers of the immune system. Previous studies have shown that in response to wound formation, ship1-deficient zebrafish have increased neutrophil motility while overexpression of ship1 resulted in decreased neutrophil migration (Lam et al. 2012). From this research, it is suggested that SHIP1 is a key brake that limits neutrophil motility through a PI3K signaling-dependent pathway. While the role of SHIP1 during a wound …

Optimization And Synthesis Of Silver Nanoparticles Embedded Within A Porous Substrate For Raman Spectroscopy, Matthew W. Talbot

Honors College

Raman spectroscopy is a promising method for detection of a wide range of water contaminants. Raman spectroscopy’s growing list of applications relies upon signal enhancement achieved in recent years. A test strip or substrate designed to optimize Raman spectra, capable of withholding water and enhancing signal, would be a useful tool for applications including water quality tests. Signal enhancement may be achieved by the addition of silver nanoparticles (NPs) into a three-dimensional structure of cellulose nanofibers (CNF). The magnitude of signal enhancement may be related to nanoparticle size and morphology, and so control over the synthesis of silver nanoparticles could …