Biomedical Engineering and Bioengineering Commons^™

Enhanced Recovery Of Mechanical Function In The Canine Heart By Seeding An Extracellular Matrix Patch With Mesenchymal Stem Cells Committed To A Cardiac Lineage., Glenn Gaudette, Irina Potapova, Sergey Doronin, Damon Kelly, Amy Rosen, Adam Schuldt, Zhongju Lu, Paul Kochupura, Richard Robinson, Michael Rosen, Peter Brink, Ira Cohen

Glenn R. Gaudette

The need to regenerate tissue is paramount, especially for the heart that lacks the ability to regenerate after injury. The urinary bladder extracellular matrix (ECM), when used to repair a right ventricular defect, successfully regenerated some mechanical function. The objective of the current study was to determine whether the regenerative effect of ECM could be improved by seeding the patch with human mesenchymal stem cells (hMSCs) enhanced to differentiate down a cardiac linage. hMSCs were used to form three-dimensional spheroids. The expression of cardiac proteins was determined in cells exposed to the spheroid formation and compared with nonmanipulated hMSCs. To …