Biomedical Engineering and Bioengineering Commons^™

Auto-Fluorescence Lifetime And Light Reflectance Spectroscopy For Breast Cancer Diagnosis: Potential Tools For Intraoperative Margin Detection, Vikrant Sharma, Shivaranjani Shivalingaiah, Yan Peng, David Euhus, Zygmunt Gryczynski, Hanli Liu

Bioengineering Faculty Publications

This study investigates the use of two spectroscopic techniques, auto-fluorescence lifetime measurement (AFLM) and light reflectance spectroscopy (LRS), for detecting invasive ductal carcinoma (IDC) in human ex vivo breast specimens. AFLM used excitation at 447 nm with multiple emission wavelengths (532, 562, 632, and 644 nm), at which autofluorescence lifetimes and their weight factors were analyzed using a double exponent model. LRS measured reflectance spectra in the range of 500-840 nm and analyzed the spectral slopes empirically at several distinct spectral regions. Our preliminary results based on 93 measured locations (i.e., 34 IDC, 31 benign fibrous, 28 adipose) from 6 …