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Articles 1 - 10 of 10

Full-Text Articles in Biomedical Engineering and Bioengineering

Getting To The Root Of Bacterial Hairs: What Is “S”?, Rebecca Gaddis, Samantha O'Conner, Evan Anderson, Terri Camesano, Nancy Burnham Dec 2015

Getting To The Root Of Bacterial Hairs: What Is “S”?, Rebecca Gaddis, Samantha O'Conner, Evan Anderson, Terri Camesano, Nancy Burnham

Nancy A. Burnham

An atomic force microscope (AFM) was used to measure the steric forces of lipopolysaccharides (LPS) on the biofilm-forming bacteria, Pseudomonas aeruginosa. It is well known that LPS play a vital role in biofilm formation. These forces were characterized with a modified version of the Alexander and de Gennes (AdG) model for polymers, which is a function of equilibrium brush length, L, probe radius, R, temperature, T, separation distance, D, and an indefinite density variable, s. This last parameter was originally distinguished by de Gennes as the root spacing or mesh spacing depending upon the type of polymer adhesion; however since ...


Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang Sep 2015

Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang

Yi Pang

Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and ...


Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang Sep 2015

Biocompatible Flavone-Based Fluorogenic Probes For Quick Wash-Free Mitochondria! Imaging In Living Cells, Bin Liu, Mickey Shah, Ge Zhang, Qin Liu, Yi Pang

Ge Zhang

Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and ...


De Novo Design Of Self-Assembled Hexapeptides As Β-Amyloid (Aβ) Peptide Inhibitors, Jie Zheng, Ge Zhang, Mingzhen Zhang, Qiuming Wang, Guizhao Liang, Mingzhen Zhang, Jun Zhao, Kunal Patel, Xiang Yu, Chao Zhao, Binrong Ding, Feimeng Zhou Sep 2015

De Novo Design Of Self-Assembled Hexapeptides As Β-Amyloid (Aβ) Peptide Inhibitors, Jie Zheng, Ge Zhang, Mingzhen Zhang, Qiuming Wang, Guizhao Liang, Mingzhen Zhang, Jun Zhao, Kunal Patel, Xiang Yu, Chao Zhao, Binrong Ding, Feimeng Zhou

Ge Zhang

The ability of peptides to construct specific secondary structures provides a useful function for biomaterial design that cannot be achieved with traditional organic molecules and polymers. Inhibition of amyloid formation is a promising therapeutic approach for the treatment of neurodegenerative diseases. Existing peptide-based inhibitors are mainly derived from original amyloid sequences, which have very limited sequence diversity and activity. It is highly desirable to explore other peptide-based inhibitors that are not directly derived from amyloid sequences. Here, we develop a hybrid high-throughput computational method to efficiently screen and design hexapeptide inhibitors against amyloid-β (Aβ) aggregation and toxicity from the first ...


3d Scaffolds In Tissue Engineering And Regenerative Medicine: Beyond Structural Templates?, Tierney Deluzio, Dawit Seifu, Kibret Mequanint Aug 2015

3d Scaffolds In Tissue Engineering And Regenerative Medicine: Beyond Structural Templates?, Tierney Deluzio, Dawit Seifu, Kibret Mequanint

Tierney GB Deluzio

The objective of this article is to systematically present the emerging understanding that 3D porous scaffolds serve not only as structural templates for tissue fabrication but also provide complex signaling cues to cells and facilitate oxygen and therapeutic agent delivery. Strategies in the field of tissue engineering and regenerative medicine often rely on 3D scaffolds to mimic the natural extracellular matrix as structural templates that support cell adhesion, migration, differentiation and proliferation, and provide guidance for neo-tissue formation. In addition to providing a temporary support for tissue fabrication, 3D scaffolds have also been used to study cell signaling that best ...


3d Scaffolds In Tissue Engineering And Regenerative Medicine: Beyond Structural Templates?, Tierney Deluzio, Dawit Seifu, Kibret Mequanint Aug 2015

3d Scaffolds In Tissue Engineering And Regenerative Medicine: Beyond Structural Templates?, Tierney Deluzio, Dawit Seifu, Kibret Mequanint

Tierney GB Deluzio

The objective of this article is to systematically present the emerging understanding that 3D porous scaffolds serve not only as structural templates for tissue fabrication but also provide complex signaling cues to cells and facilitate oxygen and therapeutic agent delivery. Strategies in the field of tissue engineering and regenerative medicine often rely on 3D scaffolds to mimic the natural extracellular matrix as structural templates that support cell adhesion, migration, differentiation and proliferation, and provide guidance for neo-tissue formation. In addition to providing a temporary support for tissue fabrication, 3D scaffolds have also been used to study cell signaling that best ...


Three-Dimensional Confocal Microscopy Indentation Method For Hydrogel Elasticity Measurement, Donghee Lee, Md Mahmudur Rahman, You Zhou, Sangjin Ryu Aug 2015

Three-Dimensional Confocal Microscopy Indentation Method For Hydrogel Elasticity Measurement, Donghee Lee, Md Mahmudur Rahman, You Zhou, Sangjin Ryu

Md Mahmudur Rahman

No abstract provided.


Integrating Top-Down And Bottom-Up Scaffolding Tissue Engineering Approach For Bone Regeneration, M. Ramalingam, Esmaiel Jabbari, S. Ramakrishna, A. Khademhosseini Mar 2015

Integrating Top-Down And Bottom-Up Scaffolding Tissue Engineering Approach For Bone Regeneration, M. Ramalingam, Esmaiel Jabbari, S. Ramakrishna, A. Khademhosseini

Esmaiel Jabbari

No abstract provided.


Bioinspired Nanomaterials For Bone Regeneration, Esmaiel Jabbari Mar 2015

Bioinspired Nanomaterials For Bone Regeneration, Esmaiel Jabbari

Esmaiel Jabbari

No abstract provided.


Nanoparticles For Stem-Cell Engineering, Esmaiel Jabbari Mar 2015

Nanoparticles For Stem-Cell Engineering, Esmaiel Jabbari

Esmaiel Jabbari

No abstract provided.