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Full-Text Articles in Biomedical Engineering and Bioengineering
Enzymatic Glycerolysis Of Soybean Oil , Hossein Noureddini, S E. Harmeier
Enzymatic Glycerolysis Of Soybean Oil , Hossein Noureddini, S E. Harmeier
Papers in Biomaterials
Enzymatic glycerolysis of soybean oil was studied. Of the nine lipases that were tested in the initial screening, Pseudomonas sp. resulted in the highest yield of monoglycerides. Lipase from Pseudomonas sp. was further studied for the influence of temperature, thermal stability, enzyme/oil ratio, and glycerol/oil ratio. A full factorial optimization approach was performed. The following conditions were tested over the specified ranges: temperature (30?70°C), thermal stability (30?70°C), enzyme/oil ratio (0.05?0.2 g enzyme/10 g oil), glycerol/oil ratio (1:1?3:1 glycerol/oil molar ratio) and 1 h reaction time. The stability of the enzyme at the reaction temperature was also incorporated as a separate …
Glycerolysis Of Fats And Methyl Esters., Hossein Noureddini, V Medikonduru
Glycerolysis Of Fats And Methyl Esters., Hossein Noureddini, V Medikonduru
Papers in Biomaterials
The glycerolysis of methyl esters and triglycerides with crude glycerol. a coproduct from the transesterification of triglycerides, was studied. Three procedures were followed ior this conversion. The first procedure was a one-step glycerolysis with methyl esters. The second procedure was a two-step process. This proced~~irnev olved an initial partial glycerolysis with methyl esters, followed by fat glycerolysis. The third procedure u,as a simultaneous glycerolysis n,ith methyl esters and triglycerides. In the glycerolysis with methyl esters, the removal of methanol is vital to the production of mono- and diglycerides. Methanol was removed either by drawing vacuum on the reactor or by …