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Co-Culture Of Smooth Muscle Cells And Endothelial Cells On Porous 3d Polyurethane Scaffolds For Vascular Tissue Engineering, Aparna Bhattacharyya
Co-Culture Of Smooth Muscle Cells And Endothelial Cells On Porous 3d Polyurethane Scaffolds For Vascular Tissue Engineering, Aparna Bhattacharyya
Electronic Thesis and Dissertation Repository
One of the challenges in the designing of clinically-relevant vascular substitutes is our lack of understanding on how vascular smooth muscle cells (VSMCs) and vascular endothelial cells (VECs) interact in the graft. The aim of this study was to examine the factors that play a role in VSMC and VEC interaction in 3D co-culture. Highly porous 3D poly(carbonate urethane) scaffolds were fabricated using a solvent casting and particulate leaching method. VSMCs and VECs were co-cultured for 48 hours. Immunofluorescence staining showed that VSMCs readily attached to the scaffold and formed dense confluent layers which facilitated the organization of VECs of …
Oxygen Delivery Strategies In Tissue-Engineering Constructs, Dawit Gezahegn Seifu
Oxygen Delivery Strategies In Tissue-Engineering Constructs, Dawit Gezahegn Seifu
Electronic Thesis and Dissertation Repository
The supply of nutrients and the removal of waste products play a major role in tissue engineering. From all the nutrients necessary for cells seeded on scaffolds for tissue regeneration, oxygen is the limiting component due to its low solubility in culture media while cells consume five to six moles of oxygen for every mole of monosaccharide. The aim of the present work was to develop different strategies to improve the supply of oxygen to human coronary artery smooth muscle cells (HCASMC) seeded on three dimensional (3D) porous biostable polyurethane scaffolds. As a springboard for the study, the measured value …