Engineering Commons^™

Increased Trans-Glycosylation Activity Of Hexosaminidases For Synthesis Of Human Milk Oligosaccharides, Jan Muschiol, Jesper Holck, Peter K. Busk, Lene Lange, Jørn D. Mikkelsen, Anne S. Meyer, Shariza B. Jamek,

Enzyme Engineering XXIV

It is well known that the composition of human breast milk differs significantly from the one of ordinary bovine milk. Especially the presence of sialylated and fucosylated oligosaccharides contributes to its health and development promoting features for newborn infants. [1] Nevertheless, not all newborns and especially premature infants sometimes cannot be breast fed for different reasons. For those children it is important that they receive a proper balanced formula product containing the above mentioned human milk oligosaccharides (HMOs). With respect to this we are developing new enzymatic routes for synthesis of sialylated and fucosylated oligosaccharides, which can be used as …

Enzyme Shielding In A Soft Organo-Silica Layer – Pharma/Biopharma Applications, Yves Dudal, Federica Richina, Lucas Durandi, Rita Correro, Patrick Shahgaldian

Enzyme Engineering XXIV

Enzymes, or biocatalysts, are specialty proteins. Like antibodies to antigens, enzymes exhibit remarkable specificity for their substrate and naturally facilitate many chemical conversions. However, the use of enzymes in the biotech industry ($2.3bn) is strongly limited by the fact that enzymes are highly sensitive like most proteins and usually not fitted to in vivo and process conditions. Many expensive genetically engineered or biosourced enzymes show remarkable properties but need to be made more robust for deployment in the health industry.

We have developed a unique procedure to fit enzymes to in vivo and process conditions. The process is initiated by …

Computational Redesign Of Transaminase Active Site, Elisa Lanfranchi, Hein J. Wijma, Dick B. Janssen, Carlos J. Ramírez, Madhurya Lutikurti

Enzyme Engineering XXIV

Aminotransferases are widely exploited in simple as well as more elaborate multi-enzymatic cascade reactions as an environmentally friendly alternative to transition metal catalysis. However, efficient selective conversion of numerous targets is a great limitation to date [1]. Attempts to improve substrate scope have been undertaken by generation and screening of large mutant libraries, which is very time-consuming and raises costs concerns [2]. Recent approaches explored the use of molecular docking of demanding substrates, followed by energy minimization and/or MD simulations [1;3]. Still, the best results have been obtained by extensive mutagenesis and screening.

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Conference Program, Pierre Monsan, Magali Remaud-Simeon

Enzyme Engineering XXIV

No abstract provided.