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Articles 1 - 7 of 7
Full-Text Articles in Engineering
An Integrated Centrifugo-Opto-Microfluidic Platform For Arraying, Analysis, Identification And Manipulation Of Individual Cells, Robert Burger, D Kurzbuch, Robert Gorkin Iii, Gregor Kijanka, Macdara Glynn, C Mcdonagh, Jens Ducree
An Integrated Centrifugo-Opto-Microfluidic Platform For Arraying, Analysis, Identification And Manipulation Of Individual Cells, Robert Burger, D Kurzbuch, Robert Gorkin Iii, Gregor Kijanka, Macdara Glynn, C Mcdonagh, Jens Ducree
Robert Gorkin III
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.
Optical Sensing System Based On Wireless Paired Emitter Detector Diode Device And Ionogels For Lab-On-A-Disc Water Quality Analysis, Monika Czugala, Robert Gorkin Iii, Thomas Phelan, Jennifer Gaughran, Vincenzo Curto, Jens Ducree, Dermot Diamond, Fernando Benito-Lopez
Optical Sensing System Based On Wireless Paired Emitter Detector Diode Device And Ionogels For Lab-On-A-Disc Water Quality Analysis, Monika Czugala, Robert Gorkin Iii, Thomas Phelan, Jennifer Gaughran, Vincenzo Curto, Jens Ducree, Dermot Diamond, Fernando Benito-Lopez
Robert Gorkin III
This work describes the first use of a wireless paired emitter detector diode device (PEDD) as an optical sensor for water quality monitoring in a lab-on-a-disc device. The microfluidic platform, based on an ionogel sensing area combined with a low-cost optical sensor, is applied for quantitative pH and qualitative turbidity monitoring of water samples at point-of-need. The autonomous capabilities of the PEDD system, combined with the portability and wireless communication of the full device, provide the flexibility needed for on-site water testing. Water samples from local fresh and brackish sources were successfully analysed using the device, showing very good correlation …
Design And Fabrication Of A Cop-Based Microfluidic Chip: Chronoamperometric Detection Of Troponin T, Llibertat Abad, Francisco Javier Del Campo, Francesc Xavier Munoz, Luis J Fernandez, Daniel Calavia, Gloria Colom, Juan P Salvador, Maria Pilar Marco, Vanessa Escamilla-Gomez, Berta Esteban-Fernandez De Avila, Susana Campuzano, Maria Pedrero, Jose M Pingarron, Neus Godino, Robert Gorkin Iii, Jens Ducree
Design And Fabrication Of A Cop-Based Microfluidic Chip: Chronoamperometric Detection Of Troponin T, Llibertat Abad, Francisco Javier Del Campo, Francesc Xavier Munoz, Luis J Fernandez, Daniel Calavia, Gloria Colom, Juan P Salvador, Maria Pilar Marco, Vanessa Escamilla-Gomez, Berta Esteban-Fernandez De Avila, Susana Campuzano, Maria Pedrero, Jose M Pingarron, Neus Godino, Robert Gorkin Iii, Jens Ducree
Robert Gorkin III
This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells formultianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05–1.0 ng/mL. This methodology achieves a detection limit of …
Fabricating Electrodes For Amperometric Detection In Hybrid Paper/Polymer Lab-On-A-Chip Devices, Neus Godino, Robert Gorkin Iii, Ken Bourke, Jens Ducree
Fabricating Electrodes For Amperometric Detection In Hybrid Paper/Polymer Lab-On-A-Chip Devices, Neus Godino, Robert Gorkin Iii, Ken Bourke, Jens Ducree
Robert Gorkin III
We present a novel, low-resource fabrication and assembly method for creating disposable amperometric detectors in hybrid paper-polymer devices. Currently, mere paper-based microfluidics is far from being able to achieve the same level of process control and integration as state-of-the-art microfluidic devices made of polymers. To overcome this limitation, in this work both substrate types are synergistically combined through a hybrid, multi-component/multi-material system assembly. Using established inkjet wax printing, we transform the paper into a profoundly hydrophobic substrate in order to create carbon electrodes which are simply patterned from carbon inks via custom made adhesive stencils. By virtue of the compressibility …
Centrifugo-Pneumatic Valving Utilizing Dissolvable Films, Robert Gorkin Iii, Charles Nwankire, Jennifer Gaughran, Xin Zhang, Gerard Donohoe, Martha Rook, Richard O'Kennedy, Jens Ducree
Centrifugo-Pneumatic Valving Utilizing Dissolvable Films, Robert Gorkin Iii, Charles Nwankire, Jennifer Gaughran, Xin Zhang, Gerard Donohoe, Martha Rook, Richard O'Kennedy, Jens Ducree
Robert Gorkin III
In this article we introduce a novel technology that utilizes specialized water dissolvable thin films for valving in centrifugal microfluidic systems. In previous work (William Meathrel and Cathy Moritz, IVD Technologies, 2007), dissolvable films (DFs) have been assembled in laminar flow devices to form efficient sacrificial valves where DFs simply open by direct contact with liquid. Here, we build on the original DF valving scheme to leverage sophisticated, merely rotationally actuated vapour barriers and flow control for enabling comprehensive assay integration with low-complexity instrumentation on "lab-on-a-disc" platforms. The advanced sacrificial valving function is achieved by creating an inverted gas-liquid stack …
Centrifugal Automation Of A Triglyceride Bioassay On A Low-Cost Hybrid Paper-Polymer Device, Neus Godino, Elizaveta Vereshchagina, Robert Gorkin Iii, Jens Ducree
Centrifugal Automation Of A Triglyceride Bioassay On A Low-Cost Hybrid Paper-Polymer Device, Neus Godino, Elizaveta Vereshchagina, Robert Gorkin Iii, Jens Ducree
Robert Gorkin III
We present a novel paper-polymer hybrid construct for the simple automation of fundamental microfluidic operations in a lab-on-a-disc platform. The novel design, we term a paper siphon, consists of chromatographic paper strips embedded along a siphon microchannel. The paper siphon relies on two main interplaying forces to create unique valving and liquid-sampling methods in centrifugal microfluidics. At sufficiently low speeds, the inherent wicking of the paper overcomes the rotationally induced centrifugal force to drive liquids towards inwards positions of the disc. At elevated speeds, the dominant centrifugal force will extract liquid from the siphon paper strip towards the edge of …
Centrifugal Microfluidics For Cell Analysis, Robert Burger, Daniel Kirby, Macdara Glynn, Charles E Nwankire, Mary O'Sullivan, Jonathan Siegrist, David Kinahan, Gerson Aguirre, Gregor Kijanka, Robert Gorkin Iii, Jens Ducree
Centrifugal Microfluidics For Cell Analysis, Robert Burger, Daniel Kirby, Macdara Glynn, Charles E Nwankire, Mary O'Sullivan, Jonathan Siegrist, David Kinahan, Gerson Aguirre, Gregor Kijanka, Robert Gorkin Iii, Jens Ducree
Robert Gorkin III
Over the past two decades, centrifugal microfluidic systems have successfully demonstrated their capability for robust, high-performance liquid handling to enable modular, multipurpose lab-on-a-chip platforms for a wide range of lifescience applications. Beyond the handling of homogeneous liquids, the unique, rotationally controlled centrifugal actuation has proven to be specifically advantageous for performing cell and particle handling and assays. In this review we discuss technologies to implement two important steps for cell handling, namely separation and capturing/counting.