Engineering Commons^™

Smart Packaging: A Novel Technique For Localized Drug Delivery For Ovarian Cancer, Eva Christabel Williams

USF Tampa Graduate Theses and Dissertations

Localized drug delivery is emerging as an effective technique due to its ability to administer therapeutic concentrations and controlled release of drugs to cancer sites in the body. It also prevents the contact of harsh chemotherapy drugs to healthy regions in the body that otherwise would become exposed to current treatments.

This study reports on a model chemotherapy drug delivery system comprising non-ionic surfactant vesicles (niosomes) packaged within a temperature-sensitive chitosan network. This smart packaging, or package-within-a package system, provides two distinct advantages. First, the gel prevents circulation of the niosomes and maintains delivery in the vicinity of a tumor. …

Determination Of Isotherms Of Enantiomers On A Chiral Stationary Phase Using Supercritical Fluid Chromatography, Wade Newlin Mack Ii

USF Tampa Graduate Theses and Dissertations

Supercritical fluids provide a robust, tunable environment with favorable transport properties and enhanced solubility. Supercritical carbon dioxide is an environmentally friendly substance that has distinct advantages to traditional solvents used in large scale chemical processing. Ibuprofen is an enantiomeric drug that has been shown to fight prostate cancer and has been used as a mild Alzheimer's disease treatment in the S conformation. Purification of high value products with decreased hazardous material consumption is accomplished by employing supercritical fluid chromatography (SFC). Large scale, single enantiomer purification of pharmaceuticals is dependent on scale-up information. Equilibrium isotherms provide the necessary information to scale-up …

Determination Of Ibuprofen Isotherm Using Supercritical Fluid Chromatography, Loi Ho

USF Tampa Graduate Theses and Dissertations

Chromatography is widely used to determine physiochemical properties data including adsorption isotherm. In the separation of enantiomers through sorptive processes, supercritical fluids allow efficient and green alternatives to liquid solvent based systems. The isotherm information is vital in the development of operating policies and design of preparative chromatographic or moving bed separation schemes. Determination of sorption isotherms from experimental chromatographic elution data is automated and compared with different chromatogram model. Procedures were developed and validated for separation of R- and S- ibuprofen with supercritical 〖CO〗_2 and ethanol mixture as the mobile phase over a chiral stationary phase. The isotherms for …

Cactus Mucilage-Assisted Heavy Metal Separation: Design And Implementation, Dawn Iona Fox

USF Tampa Graduate Theses and Dissertations

Natural contamination of groundwater by arsenic (As) has become a critical public health threat in many parts of the world. The well-known regions associated with As contamination of groundwater are Bangladesh and West Bengal, India where approximately 100 million people are exposed to high levels of arsenic by drinking arsenic-contaminated groundwater and about 35 million are already affected. Long-term drinking of arsenic-contaminated water leads to arsenicosis, which is characterized by cancers of the skin, organ disease and certain other types of cancer. Affected developing communities are at higher risk because they may not have access to conventional water treatment facilities. …

Oxygen Diffusion Characterization Of Frp Composites Used In Concrete Repair And Rehabilitation, Chandra K. Khoe

USF Tampa Graduate Theses and Dissertations

Many independent studies have conclusively demonstrated that fiber reinforced polymers (FRP) slow down chloride-induced corrosion of steel in concrete. The mechanism for this slow down is not well understood but it has been hypothesized that FRP serves as a barrier to the ingress of chloride, moisture, and oxygen that sustain electrochemical corrosion of steel.

This dissertation presents results from an experimental study that determined the oxygen permeation rates of materials used in infrastructure repair. In the study, the oxygen permeation constants for epoxy, carbon and glass fiber laminates, concrete, epoxy-concrete and FRP-concrete systems were determined and a method developed to …

Supercritical Fluid Aided Microencapsulation Of Dry Powders, Raquel Carvallo

USF Tampa Graduate Theses and Dissertations

Coating of fine particles to produce tailored surface properties is currently a key development for supercritical fluids applications, in different areas such as: pharmaceutical, nutraceutical, cosmetic, agrochemical, electronic and specialty chemistry industries. During the encapsulation process the particles surface can be designed with specific properties by spreading a thin film coating material over the surface of the particles.

Chitosan, a natural polymer, was used in this work as the encapsulant material. Chitosan is biocompatible, biodegradable to normal body constituents, safe, non-toxic, bacteriostatic, anticancerogen, and versatile polymer. These attributes are among the properties that make Chitosan an attractive component of pharmaceutical …

Catalytic Hydrodehalogenation And Hydrogenation Of Halogenated Aromatic Organic Contaminants For Application To Soil Remediation, Claire J. Osborn

USF Tampa Graduate Theses and Dissertations

The objective of this research was to aid in the development of a new method for removing and destroying soil contaminants. In particular, 1,2,4,5-tetrachlorobenzene (TeCB) was selected for this research. Hydrodehalogenation (HDH) was paired with hydrogenation for remedially destroying TeCB without generating a secondary waste stream in a single batch reactor. Palladium- and rhodium-catalyzed HDH and hydrogenation were applied in a batch reactor at room temperature and moderate hydrogen pressure. Cyclohexane was formed as an end product with benzene as an intermediate reactant.

An analytical method was developed to measure TeCB, benzene, and cyclohexane in a solution of water and …

The Conversion Of Low-Grade Heat Into Power Using Supercritical Rankine Cycles, Huijuan Chen

USF Tampa Graduate Theses and Dissertations

Low-grade heat sources, here defined as below 300 ºC, are abundantly available as industrial waste heat, solar thermal, and geothermal, to name a few. However, they are under-exploited for conversion to power because of the low efficiency of conversion. The utilization of low-grade heat is advantageous for many reasons. Technologies that allow the efficient conversion of low-grade heat into mechanical or electrical power are very important to develop.

This work investigates the potential of supercritical Rankine cycles in the conversion of low-grade heat into power. The performance of supercritical Rankine cycles is studied using ChemCAD linked with customized excel macros …

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …

The Virtual Hip: An Anatomically Accurate Finite Element Model Based On The Visible Human Dataset, Jonathan M. Ford

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to determine if element decimation of a 3-D anatomical model affects the results of Finite Element Analysis (FEA). FEA has been increasingly applied to the biological and medical sciences. In order for an anatomical model to successfully run in FEA, the 3-D model’s complex geometry must be simplified, resulting in a loss of anatomical detail. The process of decimation reduces the number of elements within the structure and creates a simpler approximation of the model. Using the National Library of Medicine’s Visible Human Male dataset, a virtual 3-D representation of several structures of the …

Investigation Of Buildup Dose For Therapeutic Intensity Modulated Photon Beams In Radiation Therapy, Khosrow Javedan

USF Tampa Graduate Theses and Dissertations

Buildup dose of Mega Voltage (MV) photon beams can be a limiting factor in intensitymodulated radiation therapy (IMRT) treatments. Excessive doses can cause patient discomfort and treatment interruptions, while underdosing may lead to local failure.

Many factors which contribute to buildup dose, including the photon beam energy spectrum, scattered or contaminant radiation and their angular distribution, are not modeled well in commercial treatment planning systems. The accurate Monte Carlo method was employed in the studies to estimate the doses.

Buildup dose of 6MV photon beams was investigated for three fundamentally different IMRT modalities: between Helical TomoTherapy and traditional opposed tangential …

Synthesis, Characterization, And Self-Assembly Of Gold Nanorods And Nanoprisms, Kristina L. Tran

USF Tampa Graduate Theses and Dissertations

The unique properties of gold nanoparticles make them excellent candidates for applications in electronics, sensing, imaging, and photothermal therapy. Though abundant literature exists for isotropic gold nanoparticles, work on nanoparticles of different shapes has been gaining interest recently. Anisotropic gold nanoparticles, such as nanorods and nanoprisms, have tunable optical properties in the visible and near-infrared regions. Through synthesis and surface modification, the production of various shapes of these gold nanoparticles can be controlled to meet different applications.

Two different types of gold nanorods were used in this thesis. The first type was stabilized with cetyltrimethylammonium bromide (CTAB) and had aspect …

Effects Of Monoclonal Anti-Abeta Antibodies On The Amyloid Beta Peptide Fibrillogenesis And Their Involvement In The Clearance Of Alzheimer's Disease Plaques, Jeffy Pilar Jimenez

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is the most common cause of senile dementia worldwide. AD is a neurodegenerative disorder characterized by the loss of memory and language skill, collapse of the cognitive function, and distortion of social behavior. As of today, the onset mechanisms of AD and cure are unknown; however, three hallmarks are commonly encountered: extra and intracellular accumulation of amyloid beta (A!) peptide plaques, formation of intracellular neurofibrillary tangles, and inevitable neuronal death. Hypothetically, a possible scenario provoking or involved in the onset of AD is a cascade effect that starts with an imbalance in the production and clearance of …

Pegylation Of Niosomes, John A. Elliott

USF Tampa Graduate Theses and Dissertations

The research presented in this dissertation describes the creation and characterization of a novel antibody-vesicle conjugate modified with polyethylene glycol (PEG) that possesses enhanced binding to and uptake by inflammation-activated endothelial cells with improved storage stability and longer shelf-life and potential reduction in immunogenic potential compared to previous designs.

Targeted drug delivery provides an effective means of delivering therapeutic concentrations of a drug to the site or organ of action. The drug is delivered using a niosome, a vesicle with an aqueous core and a bilayer membrane composed of non-ionic surfactants and cholesterol. Antibodies that recognize specific cell antigens are …

Thermodynamic Model And The Controlling Variables Of Phosphate Lattice Loss, Mohammad Abutayeh

USF Tampa Graduate Theses and Dissertations

A thermodynamic model was developed based upon five equilibrium reactions to predict the limits of distribution of phosphates between the liquid and the solid phases in a reactor used to extract phosphoric acid from phosphate rock. A computer code was generated to carry out different simulations of the model using several inputs of temperatures and liquid phase sulfuric acid contents. Ideal Solution, Debye-Hückel, and Robinson-Guggenheim-Bates electrolyte activity coefficient models were employed alternately in each simulation to complete the thermodynamic model and the outputs were compared to one another.

Experimental data of equilibrium constants were regressed to adjust the values of …