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Closure Of Earthern Manure Structures (Including Basins, Holding Ponds And Lagoons), Don D. Jones, Richard K. Koelsch, Saqib Mukhtar, Ronald Sheffield, John W. Worley

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper is a summary of what is known scientifically about the closure of earthen manure structures without artificial liners, including lagoons, storage basins, and runoff holding ponds, and what needs to be examined further to increase our understanding of the dynamics of closing them in an environmentally safe manner. This information should be useful as a guide for state regulatory agencies considering rules for closure and for academicians and consultants who work with livestock production facilities.

Detection And Neutralization Of Bacterial Biohazards Via Nanoencapsulation, Rosaura De Zayas

FIU Electronic Theses and Dissertations

Bacteria biohazards, such as Anthrax, are responsible for causing mild to serious illnesses in humans and animals. The primary aim of this research study was to develop a rapid one step assay to detect and neutralize bacteria-based biohazards, using an Immunoliposome-nanoparticle complex. An Anthrax model, Bacillus cereus, was grown for 3 hours and diluted 1:25 in media (2.0 x 107 cfu/ml). The Bacillus cereus was interacted with an Immunoliposome-nanoparticle complex containing an MgO-C 2 neutralization agent. The samples were analyzed via flow cytometry with a 1:8:1 ratio Bacillus cereus, Immunoliposome-nanoparticle complex, and Ethidium homodimer-1 for two hours. The results obtained …

Pre- And Post-Alpha Motoneuronal Control Of The Soleus H-Reflex During Sinusoidal Hip Movements In Human Spinal Cord Injury, Maria Knikou, Debjani Chaudhuri, Elizabeth Kay, Brian D. Schmit

Biomedical Engineering Faculty Research and Publications

The aim of this study was to establish the contribution of hip-mediated sensory feedback to spinal interneuronal circuits during dynamic conditions in people with incomplete spinal cord injury (SCI). Specifically, we investigated the effects of synergistic and antagonistic group I afferents on the soleus H-reflex during imposed sinusoidal hip movements. The soleus H-reflex was conditioned by stimulating the common peroneal nerve (CPN) at short (2, 3, and 4 ms) and long (80, 100, and 120 ms) conditioning test (C-T) intervals to assess the reciprocal and pre-synaptic inhibition of the soleus H-reflex, respectively. The soleus H-reflex was also conditioned by medial …

Department Of Biological Systems Engineering Newsletter, Issue 1, August 2006

BSE Department Magazine

Contents:
Welcome Comments from Department Head
Student Poster Exhibition
Quarter-scale Tractor Team
Distinguished Fellowship Awards
Scoops and Scholars
Educational Objectives
ASABE Honors Department Faculty and Staff Members
Computer Tool Helps Crop Production under Limited Irrigation
Comings and Goings
Milestones
Faculty and Staff News
Alumni Update
Alumni Profile
Graduation
UCare Spring 2006 Undergraduate Research
MSYM Student on Winning Team
A Nebraskan Goes Way South
Visitors to BSE

99mTc-Labeled C2a Domain Of Synaptotagmin I As A Target-Specific Molecular Probe For Noninvasive Imaging Of Acute Myocardial Infarction, Ming Zhao, Xiaoguang Zhu, Shundong Ji, Jundong Zhou, Kutlan S. Ozker, Wei Fang, Robert C. Molthen, Robert S. Hellman

Biomedical Engineering Faculty Research and Publications

Abstract: The exposure of phosphatidylserine (PtdS) is a common molecular marker for both apoptosis and necrosis and enables the simultaneous detection of these distinct modes of cell death. Our aim was to develop a radiotracer based on the PtdS-binding activity of the C2A domain of synaptotagmin I and assess ^99mTc-C2A-GST (GST is glutathione S-transferase) using a reperfused acute myocardial infarction (AMI) rat model. Methods: The binding of C2A-GST toward apoptosis and necrosis was validated in vitro. After labeling with ^99mTc via 2-iminothiolane thiolation, radiochemical purity and radiostability were tested. Pharmacokinetics and biodistribution were studied in healthy rats. …

Vegetative Treatment Systems For Open Lot Runoff: Review Of Literature, Richard K. Koelsch, Jeffrey Lorimer, Kyle Mankin

Department of Biological Systems Engineering: Conference Presentations and White Papers

Runoff from open lot livestock systems (beef and dairy) defined as Concentrated Animal Feeding Operations (CAFO) must be controlled by systems designed and managed to prevent the release of manure-contaminated runoff for storms equal to or less than a 25-year, 24-hour design storm. This performance standard has been attained for open lot systems with some combination of clean water diversion, settling basins, runoff collection ponds and irrigation systems (baseline system).

An alternative approach is to rely on overland flow and infiltration into cropland with perennial forage or grasses for treatment of open lot runoff. Such vegetative systems have been researched …

Environmental Effects On Driver Acceleration Exposure, Shekhar Khanal

Masters Theses

The European Union has developed a set of standard for the exposure to whole body vibration measured by acceleration. For manufacturers to meet this directive, it is essential to understand the levels of exposures that are common among long-haul vehicle drivers.

Many factors can influence the exposure levels. Generally, all the European vehicles are the cab-over design. Each vehicle manufacturer has provided a combination of truck and cab suspensions in addition to the air-ride seating that is standard on the vehicles. The road quality, driver speeds, and time of daily work can have variation from country to country. The determination …

Precision And Accuracy Of Thermal Calibration Of Atomic Force Microscopy Cantilevers, Nancy Burnham, G Matei, E Thoreson, J Pratt, D Newell

Nancy A. Burnham

To have confidence in force measurements made with atomic force microscopes(AFMs), the spring constant of the AFM cantilevers should be known with good precision and accuracy, a topic not yet thoroughly treated in the literature. In this study, we compared the stiffnesses of uncoated tipless uniform rectangular silicon cantilevers among thermal, loading, and geometric calibration methods; loading was done against an artifact from the National Institute of Standards and Technology (NIST). The artifact was calibrated at NIST using forces that were traceable to the International System of units. The precision and accuracy of the thermal method were found to be …

Vascular Reactivity Of Isolated Rat Mesenteric Arterioles In The Presence And Absence Of Ouabain, Rohit Chawla

FIU Electronic Theses and Dissertations

The microvasculature plays a significant role in the regulation of blood pressure and regional blood supply. Cardiotonic steroids like the adrenal cortical hormone (ouabain) have been proposed to play a role in some forms of hypertension. The purpose of this study was to determine the effect of different agonists on arteriolar diameter in the presence and absence of ouabain.

In Vitro studies on isolated intact rat mesenteric arterioles were performed by administering different concentrations of the vasoconstrictor norepinephrine (NE) and the vasorelaxant acetylcholine (Ach) in the presence and absence of ouabain. NE induced constriction was not significantly enhanced in the …

Yield Response Of Corn To Deficit Irrigation In A Semiarid Climate, José O. Payero, Steven R. Melvin, Suat Irmak, David D. Tarkalson

Biological Systems Engineering: Papers and Publications

Irrigation water supplies are decreasing in many areas of the U.S. Great Plains, which is requiring many farmers to con¬sider deficit-irrigating corn (Zea mays L.) or growing crops like winter wheat (Triticum aestivum L.) that require less water, but that are less profitable. The objectives of this study were to: (1) quantify the yield response of corn to deficit irrigation, and (2) determine which of several seasonal water variables correlated best to corn yield in a semiarid climate. Eight (T1–T8) and nine (T1–T9) deficit-irrigated treatments (including dryland), were compared in 2003 and 2004 in North Platte, Nebraska. The …

Economics Of Manure Phosphorus Distribution From Beef Feeding Operations, William F. Kissinger, Raymond E. Massey, Richard K. Koelsch, Galen E. Erickson

Department of Biological Systems Engineering: Conference Presentations and White Papers

An economic model was developed to evaluate cost and value of manure distribution. Feedlots ranging in size from 2,500 head to 25,000 head one-time capacities were used as case studies to calculate excretion amounts from cattle fed diets with a range of phosphorus. Diet P and subsequent costs of distributing the manure were used to analyze the corresponding costs of manure distribution, in addition to determining the required available land needed to be in compliance with a nutrient management plan based on utilization of manure P by the crops grown.

The model illustrated when animals are fed diets of increasing …

National Livestock And Poultry Environmental Learning Center, Richard K. Koelsch, Mark Risse, Joe Harrison, Jill Heemstra, Frank Humenik

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper describes a new national initiative to improve the delivery of science-based information to non-research customers. The vision of the Livestock and Poultry Environmental Learning Center is to provide individuals involved in public policy issues, animal production, and delivery of technical services for confined animal systems with on-demand access to the nation’s best science-based resources that is responsive to priority and emerging environmental issues associated with animal agriculture. This paper describes the implementation plans for this project.

Bme Annual Report 2005-2006, Department Of Biomedical Engineering, Florida International University

Biomedical Engineering Department Annual Reports

No abstract provided.

Molecular Modeling Of Proteins And Peptides Related To Cell Attachment In Vivo And In Vitro, Wanhua Zhao

Doctoral Dissertations

Polypeptides constitute half of the dry mass of the cell, they form the bulk of the extracellular matrix (ECM), and they are a common element of extra- and intracellular signaling pathways. There is increasing interest in the development of computational methods in polypeptide and protein engineering on all length scales. This research concerns the development of computational methods for study of polypeptide interactions related to cell attachment in vivo and in vitro.

Polypeptides are inherently biocompatible, and an astronomical range of unique sequences can be designed and realized in massive quantities by modern methods of synthesis and purification. These …

Critical Computational Aspects Of Near Infrared Circular Tomographic Imaging: Analysis Of Measurement Number, Mesh Resolution And Reconstruction Basis, Phaneendra K. Yalavarthy, Hamid Dehghani, Brian W. Pogue, Keit D. Paulsen

Dartmouth Scholarship

The image resolution and contrast in Near-Infrared (NIR) tomographic image reconstruction are affected by parameters such as the number of boundary measurements, the mesh resolution in the forward calculation and the reconstruction basis. Increasing the number of measurements tends to make the sensitivity of the domain more uniform reducing the hypersensitivity at the boundary. Using singular-value decomposition (SVD) and reconstructed images, it is shown that the numbers of 16 or 24 fibers are sufficient for imaging the 2D circular domain for the case of 1% noise in the data. The number of useful singular values increases as the logarithm of …

Benefits Of Automatic Differentiation For Biomechanical Optimizations, Jeffrey A. Reinbolt, B. J. Fregly

Jeffrey A. Reinbolt

No abstract provided.

Cosmm: An Undergraduate Laboratory For Engineering And Manufacturing Complex, Organic Shapes Using Nature As A Template, Daniel Walsh, Lanny Griffin, Robert Crockett

Biomedical Engineering

No abstract provided.

Alterations In Regional Vascular Geometry Produced By Theoretical Stent Implantation Influence Distributions Of Wall Shear Stress: Analysis Of A Curved Coronary Artery Using 3d Computational Fluid Dynamics Modeling, John F. Ladisa, Lars E. Olson, Douglas Anthony Hettrick, David C. Warltier, Judy R. Kersten, Paul S. Pagel

Biomedical Engineering Faculty Research and Publications

Background

The success of stent implantation in the restoration of blood flow through areas of vascular narrowing is limited by restenosis. Several recent studies have suggested that the local geometric environment created by a deployed stent may influence regional blood flow characteristics and alter distributions of wall shear stress (WSS) after implantation, thereby rendering specific areas of the vessel wall more susceptible to neointimal hyperplasia and restenosis. Stents are most frequently implanted in curved vessels such as the coronary arteries, but most computational studies examining blood flow patterns through stented vessels conducted to date use linear, cylindrical geometric models. It …

Dissociation Of Automatic And Strategic Lexical-Semantics: Functional Magnetic Resonance Imaging Evidence For Differing Roles Of Multiple Frontotemporal Regions, Brian T. Gold, David A. Balota, Sara J. Jones, David K. Powell, Charles D. Smith, Anders H. Andersen

Neuroscience Faculty Publications

Behavioral research has demonstrated three major components of the lexical-semantic processing system: automatic activation of semantic representations, strategic retrieval of semantic representations, and inhibition of competitors. However, these component processes are inherently conflated in explicit lexical-semantic decision tasks typically used in functional magnetic resonance imaging (fMRI) research. Here, we combine the logic of behavioral priming studies and the neurophysiological phenomenon of fMRI priming to dissociate the neural bases of automatic and strategic lexical-semantic processes across a series of three studies. A single lexical decision task was used in all studies, with stimulus onset asynchrony or linguistic relationship between prime and …

High-Resolution Thin-Film Device To Sense Texture By Touch, Vivek C. Maheshwari, Ravi F. Saraf

Ravi Saraf Publications

Touch (or tactile) sensors are gaining renewed interest as the level of sophistication in the application of minimum invasive surgery and humanoid robots increases. The spatial resolution of current large-area (greater than 1 cm²) tactile sensor lags by more than an order of magnitude compared with the human finger. By using metal and semiconducting nanoparticles, a ~100-nm-thick, large-area thin-film device is self-assembled such that the change in current density through the film and the electroluminescent light intensity are linearly proportional to the local stress. A stress image is obtained by pressing a copper grid and a United States …

The Effect Of Physiological Cyclic Stretch On The Cell Morphology, Cell Orientation And Protein Expression Of Endothelial Cells, V. Barron, Claire Brougham, K. Coghlan, C. Stenson-Cox, D. O'Mahoney, P.E. Mchugh

Articles

In vivo, endothelial cells are constantly exposed to pulsatile shear and tensile stresses. The main aim of this study was to design and build a physiological simulator, which reproduced homogenous strain profiles of the tensile strain experienced in vivo, and to investigate the effect of this cyclic tensile strain on the cell morphology, cell orientation and protein expression of endothelial cells. The biological response of human umbilical vein endothelial cells to a uniaxial cyclic stretch, in this newly developed simulator, was examined experimentally using immunohistostaining and confocal imaging and it was

found that the cells elongated and oriented at 58.9± …

Application Of Aspen Mads-Box Genes To Alter Reproduction And Development In Trees, Gopi Krishna Podila, Leland James Cseke, Banalata Sen, David F. Karnosky

Michigan Tech Patents

The present invention provides compositions and methods for producing a transgenic plant that exhibits altered characteristics resulting from over expression or under expression of a novel polypeptide PtM3 or its homolog PtM4. The altered characteristics resulting from over-expression include at least one of the ability to convert axillary mer- istem to floral meristem; to accelerate flowering i.e., early flowering; to increase fruit production; to increase nut production; to increase seed output; to increase branching; to increase flower production; to increase fruit yield; to increase flower yield and a combination thereof. The altered characteristics resulting from suppressed expression include at least …

Imaging Breast Adipose And Fibroglandular Tissue Molecular Signatures By Using Hybrid Mri-Guided Near-Infrared Spectral Tomography, Ben Brooksby, Brian W. Pogue, Shudong Jiang, Hamid Dehghani, Subhadra Srinivasan, Christine Kogel, Tor D. Tosteson, John Weaver, Steven P. Poplack, Keith D. Paulsen

Dartmouth Scholarship

Magnetic resonance (MR)-guided near-infrared spectral tomography was developed and used to image adipose and fibroglandular breast tissue of 11 normal female subjects, recruited under an institutional review board-approved protocol. Images of hemoglobin, oxygen saturation, water fraction, and subcellular scattering were reconstructed and show that fibroglandular fractions of both blood and water are higher than in adipose tissue. Variation in adipose and fibroglandular tissue composition between individuals was not significantly different across the scattered and dense breast categories. Combined MR and near-infrared tomography provides fundamental molecular information about these tissue types with resolution governed by MR T1 images.

Explant Analysis Of Total Disc Replacement, Paul A. Anderson, Steven M. Kurtz, Jeffrey M. Toth

Biomedical Engineering Faculty Research and Publications

Explant analysis of human disc prostheses allow early evaluation of the host response to the prosthesis and the response of the prosthesis from the host. Furthermore, early predictions of failure and wear can be obtained. Thus far, about 2-3% of disc prostheses have been removed. Observed wear patterns are similar to that of appendicular prostheses including abrasions/scratching, burnishing, surface deformation, fatigue, and embedded debris. Chemically the polymeric components have shown little degradation in short-term implantation. In metal on metal prostheses the histologic responses consist of large numbers of metallic particles with occasional macrophages and giant cells. Only rare cases of …

The Antibacterial Effects Of Zinc Ion Migration From Zinc-Based Glass Polyalkenoate Cements, D. Boyd, H. Li, D. A. Tanner, Mark R. Towler, J. G. Wall

Chemical and Biochemical Engineering Faculty Research & Creative Works

Zinc-based glass polyalkenoate cements have been synthesised and their potential use in orthopaedic applications investigated. Zinc ions were released from the materials in a rapid burst over the first 24 h after synthesis, with the release rate falling below detectable levels after 7 days. Cement-implanted bone samples were prepared, and the released zinc was shown, using energy dispersive X-ray analysis, to penetrate from the cement into the adjacent bone by up to 40 μm. Finally, the cements exhibited antibacterial activity against Streptococcus mutans and Actinomyces viscosus that reflected the pattern of zinc release, with the inhibition of growth greatest shortly …

A Prototype Table-Top Inverse-Geometry Volumetric Ct System, Taly Gilat Schmidt, Josh Star-Lack, N. Robert Bennett, Samuel R. Mazin, Edward G. Solomon, Rebecca Fahrig, Norbert J. Pelc

Biomedical Engineering Faculty Research and Publications

A table-top volumetric CT system has been implemented that is able to image a 5-cm-thick volume in one circular scan with no cone-beam artifacts. The prototype inverse-geometry CT (IGCT) scanner consists of a large-area, scanned x-ray source and a detector array that is smaller in the transverse direction. The IGCT geometry provides sufficient volumetric sampling because the source and detector have the same axial, or slice direction, extent. This paper describes the implementation of the table-top IGCT scanner, which is based on the NexRay Scanning-Beam Digital X-ray system (NexRay, Inc., Los Gatos, CA) and an investigation of the system performance. …

Osteogenic Differentiation Of Human Mesenchymal Stem Cells On Thin Film Tyrosine Derived Polycarbonates, Tamunotoyne Briggs

Theses

Mesenchymal stem cells, harvested from adult bone marrow, are promising in the field of regenerative medicine because of the vast differentiation potential into various cell lines such as: osteoblasts, chondrocytes, adipocytes, and neurons. Osteogenic differentiation of human mesenchymal stem cells (hMSC) could be an important tool in the treatment of orthopedic deficiencies such as bone defects. The extent of in vitro human mesenchymal stem cell growth, adhesion, motility and differentiation into osteoblasts is a function of the material surface chemistry which is mediated by protein adsorption onto the surface. A library of tyrosine derived polycarbonates allows the tailoring of material …

Pda Based Ambulatory Pulse Oximeter, Arpita Shah

Theses

The main aim of the research was to develop an ambulatory pulse oximeter which can be used to monitor the SpO₂ heart rate (HR) and plethsymograph (PPG) waveform of a person. To achieve this, an algorithm was developed in LabVIEW 8.0 to extract the HR, SpO₂ and PPG data from a Nonin Xpod device, Nonin Medical, Inc. LabVIEW PDA software was developed to make it compatible with the PDA. LabVIEW software was also developed for sending the data to the PDA via Bluetooth Pulse Oximeter commercialized by Nonin Medical Company.

Using this algorithm, data was collected from three …

The Complex Behavior Of Poly(Dte) Carbonate And Its Blend Properties, Christopher George Elvin

Theses

Over the years a number of different polymers have been studied for bone applications. Polyesters such as the poly lactic acids (PLA), poly glycolic acids (PGA), and their copolymers have been studied intensely for bone regeneration purposes. Poly(DTE)carbonate has been found useful for bone regenerative purposes and is under review by the U.S. Food and Drug Administration.

The purpose of this study was to investigate the properties of poly(DTE)carbonate when PLLA, PLGA, and P(LcoDL)LA was blended with it. The desired outcome was to produce miscible polymer blends or significant phase shift in the blended materials which will make them at …

Automatic Detection Of Qt And Related Intervals, Divya Gowdar

Theses

The objective of this thesis is divided into two main sections: The first section comprises of the development of the algorithm for the detection of the Q wave, Tmax afld the Tend to measure the intervals such as QT, QT corrected (QTc), RT, QT_max and RT_max respectively. The second section deals with the analysis of different variabilities including heart rate variability (HRV), QT, QTc, RT, QT_max and RT_max.

Using the R wave points as reference points, the Q wave was detected by using the Differential Threshold Method (DTH). The Tmax was detected by using a …