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Behavioral And Disease Ecology Of Gopher Tortoises (Gopherus Polyphemus) Post Exclusion And Relocation With A Novel Approach To Homing Determination, Johnathan Napier Jan 2018

Behavioral And Disease Ecology Of Gopher Tortoises (Gopherus Polyphemus) Post Exclusion And Relocation With A Novel Approach To Homing Determination, Johnathan Napier

Electronic Theses and Dissertations

In the wake of human expansion, relocations and the loss of habitat can be stressful to an organism, plausibly leading to population declines. The gopher tortoise (Gopherus polyphemus) is a keystone species that constructs burrows it shares with 362 commensal species. Frequent exclusions and relocations and long generation times have contributed to G. polyphemus being State-designated as Threatened in Florida. Prior studies have indicated that G. polyphemus may possess homing behavior and thus be able to counteract stressors due to relocation and exclusion. I radiotracked a cohort of G. polyphemus for 11 months following excavation, relocation, and exclusion due to …


Rebuilt And Remade: The Florida Citrus Industry, 1909-1939, James Padgett Jan 2018

Rebuilt And Remade: The Florida Citrus Industry, 1909-1939, James Padgett

Electronic Theses and Dissertations

Prior to orange juice concentrate, Florida citrus was already an industrialized agricultural sector. This thesis explores the early-20th-century Florida citrus industry and demonstrates that contemporary farming practices were influential in advancing how citrus was produced, processed, worked, marketed, and regulated in early-20th-century Florida. Restarted after devastating freezes in 1894-1895, resolute Florida growers rebuilt their groves into marvels of large-scale citrus fruit production. Continuing a legacy in experimental crossbreeding, improved varieties of citrus were developed to lengthen the season and markets. Advocated by nurserymen and university educators, biological innovation helped the citrus thrive in the 1910s and 1920s from adverse weather …


Evolution And Distribution Of Phenotypic Diversity In The Venom Of Mojave Rattlesnakes (Crotalus Scutulatus), Jason Strickland Jan 2018

Evolution And Distribution Of Phenotypic Diversity In The Venom Of Mojave Rattlesnakes (Crotalus Scutulatus), Jason Strickland

Electronic Theses and Dissertations

Intraspecific phenotype diversity allows for local adaption and the ability for species to respond to changing environmental conditions, enhancing survivability. Phenotypic variation could be stochastic, genetically based, and/or the result of different environmental conditions. Mojave Rattlesnakes, Crotalus scutulatus, are known to have high intraspecific venom variation, but the geographic extent of the variation and factors influencing venom evolution are poorly understood. Three primary venom types have been described in this species based on the presence (Type A) or absence (Type B) of a neurotoxic phospholipase A2 called Mojave toxin and an inverse relationship with the presence of snake venom metalloproteinases …


Acute Effects Of Placebo And Open-Label Placebo Treatments On Muscle Strength, Voluntary Activation, And Neuromuscular Fatigue., Alina Swafford Jan 2018

Acute Effects Of Placebo And Open-Label Placebo Treatments On Muscle Strength, Voluntary Activation, And Neuromuscular Fatigue., Alina Swafford

Electronic Theses and Dissertations

Placebo treatments have long been used to study the psychological effects of expectancy and conditioning on an inert intervention. Interestingly, open-label placebo treatments (i.e., directly telling subjects they are receiving an inactive intervention) have recently shown promise in minimizing pain in clinical patient populations. We utilized a repeated measures design to examine the acute effects of placebo, open-label placebo, and control treatments on muscle strength and voluntary activation (Experiment #1), as well as neuromuscular fatigue (Experiment #2). Twenty-one untrained males (n=11) and females (n=10) visited the laboratory on three occasions to receive each treatment in a randomized, counter-balanced manner. All …


Psychomotor Skill Measurement Of Video Game Players, Thomas Carbone Jan 2018

Psychomotor Skill Measurement Of Video Game Players, Thomas Carbone

Electronic Theses and Dissertations

Psychomotor skills are a combination of innate abilities as well as skills developed because of repeated actions. Researchers have dedicated many studies to understand the extent to which past videogame play contributes to psychomotor skills and fine motor control dexterity. However, not all gamers are created equal. With today's proliferation of platforms, many people are gamers who never pick up a controller. Grouping all gamers together forms dangerous confounds when trying to generalize across a population as diverse as today's gamers. The current study aims to study a population comprised only of gamers to see if there are significant differences …


Embryonic Stem Cell-Derived Exosomes Inhibit Doxorubicin-Induced Pyroptosis In Cell Culture Models, Zahra Tavakoli Dargani Jan 2018

Embryonic Stem Cell-Derived Exosomes Inhibit Doxorubicin-Induced Pyroptosis In Cell Culture Models, Zahra Tavakoli Dargani

Electronic Theses and Dissertations

Doxorubicin (Dox) is a potent chemotherapeutic drug used for the treatment of various cancers. Unfortunately, its use is limited as Dox induces adverse cardiotoxicity (DIC) and muscle toxicity (DIMT), which are mediated through oxidative stress, ER stress, and inflammation. However, it remains unknown whether Dox induces an inflammation mediated cell death, called “pyroptosis”. The current study is designed to determine whether Dox induces pyroptosis in cardiac and muscle cell culture models. Moreover, the protective effects of embryonic stem cell-derived exosomes (ES-Exos) in inhibiting pyroptosis will also be determined. For this purpose, we designed two different cell culture models using H9c2 …


The Identification And Segmentation Of Astrocytoma Prior To Critical Mass, By Means Of A Volumetric/Subregion Regression Analysis Of Normal And Neoplastic Brain Tissue, Lyn Higgins Jan 2018

The Identification And Segmentation Of Astrocytoma Prior To Critical Mass, By Means Of A Volumetric/Subregion Regression Analysis Of Normal And Neoplastic Brain Tissue, Lyn Higgins

Electronic Theses and Dissertations

As the underlying cause of Glioblastoma Multiforme (GBM) is presently unclear, this research implements a new approach to identifying and segmenting plausible instances of GBM prior to critical mass. Grade-IV Astrocytoma, or GBM, is an aggressive and malignant cancer arising from star-shaped glial cells, or astrocytes, where the astrocytes, functionally, assist in the support and protection of neurons within the central nervous system and spinal cord. Subsequently, our motivation for researching the ability to recognize GBM is that the underlying cause of the mutation is presently unclear, leading to the operative that GBM is only detectable through a combination of …


Split Deoxyribozyme Probe For Efficient Detection Of Highly Structured Rna Targets, Sheila Raquel Solarez Jan 2018

Split Deoxyribozyme Probe For Efficient Detection Of Highly Structured Rna Targets, Sheila Raquel Solarez

Honors Undergraduate Theses

Transfer RNAs (tRNAs) are known for their role as adaptors during translation of the genetic information and as regulators for gene expression; uncharged tRNAs regulate global gene expression in response to changes in amino acid pools in the cell. Aminoacylated tRNAs play a role in non-ribosomal peptide bond formation, post-translational protein labeling, modification of phospholipids in the cell membrane, and antibiotic biosynthesis. [1] tRNAs have a highly stable structure that can present a challenge for their detection using conventional techniques. [2] To enable signal amplification and lower detection limits, a split probe - split deoxyribozyme (sDz or BiDz) probe, which …


Bacteria That Resist Centrifugal Force, Nickolas Kessler Jan 2018

Bacteria That Resist Centrifugal Force, Nickolas Kessler

Honors Undergraduate Theses

Our lab discovered that approximately 1 in 10,000 Escherichia coli cells in stationary phase remain in suspension after a high g-force centrifuge event. To establish the mechanism behind this curious phenotype, multiple mutant strains of E. coli were independently evolved such that the majority of their populations resisted migration when exposed to high centrifugal forces. Genomic DNA sequencing of the mutants' revealed unique, isolated mutations in genes involved in capsule synthesis and exopolysaccharide (EPS) production. Each mutant exhibits a novel mechanism that allows them to remain in suspension. The mutants were further characterized by determining their growth rates, strengths …


Investigation Of Aminoglycoside Induced Nanoparticle Self-Assemblies, Michael Leong Jan 2018

Investigation Of Aminoglycoside Induced Nanoparticle Self-Assemblies, Michael Leong

Honors Undergraduate Theses

Aminoglycosides are a group of broad-spectrum antibiotics that, under neutral pH conditions, carry a positive charge. The net cationic charge arises from the high number of amino groups in the core structure of aminoglycosides. Previous studies performed have shown that negatively charged citrate ligand-capped gold nanoparticles (AuNPs) can interact with various biomolecules such as aminoglycosides. AuNPs bound to biomolecules have been used in conjugation with various assaying techniques to detect and study compounds in vitro and in vivo. AuNPs also have strong light scattering properties that can be used with a wide variety of imaging and assaying techniques. Our laboratory …