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Articles 31 - 60 of 1217
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Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Turkish Journal of Biology
Therapeutic proteins have shown to be effective against a variety of diseases. Pharmaceutical companies are progressively focusing on research using proteins in search of novel effective therapeutics. The significant attention by researchers has resulted in considerable advances in the production and usage of therapeutic proteins in recent years. In the current study we focused on the understanding of therapeutic proteins and their impact on the human health care system. Advancements in the field of biotechnology have increased and facilitated the production of therapeutically significant proteins to combat various fatal diseases. Although protein-based therapeutics have taken center stage in drug discovery …
Molecular Characterization Of Diverse Wheat Germplasm For Puroindolineproteins And Their Antimicrobial Activity, Vishal Chugh, Kirandeep Kaur, Dharmendra Singh, Vinod Kumar, Harinderjit Kaur, Harcharan Singh Dhaliwal
Molecular Characterization Of Diverse Wheat Germplasm For Puroindolineproteins And Their Antimicrobial Activity, Vishal Chugh, Kirandeep Kaur, Dharmendra Singh, Vinod Kumar, Harinderjit Kaur, Harcharan Singh Dhaliwal
Turkish Journal of Biology
Thirty different wheat genotypes, including landraces, cultivars, and related wild species, were analyzed for puroindoline genes and proteins. Full-length Pina and Pinb genes were amplified using gene-specific markers where 13 genotypes amplified both genes, while 17 genotypes had either one or none of the puroindoline genes. None of the puroindoline genes were detected in any of the Triticum durum cultivars. Genotypes with both puroindoline genes had a soft grain texture, while other genotypes had hard grains. Related nonprogenitor species with A, C, M, U, and S genomes also showed the presence of either one or both the puroindoline genes, indicating …
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Turkish Journal of Biology
Capsaicin possesses cytotoxic/anticancer activity and shares some common structural features, including a benzene ring and a long hydrophobic carbon tail, with the histone deacetylase (HDAC) inhibitors trichostatin A and suberoylanilide hydroxamic acid. The aims of this study were to investigate HDAC inhibitory and cytotoxic activities of a synthetic derivative of capsaicin, hydroxycapsaicin (6-hydroxy-N-(4-hydroxy-3-methoxybenzyl)-8-methylnonenamide), in colon cancer cell lines. Hydroxycapsaicin inhibited HDAC activity in vitro (IC50 = 72 µM) much more effectively than the prototype capsaicin (IC50 > 13.1 mM) and also exhibited HDAC inhibitory activity in human cells (HeLa cells). MTT assay demonstrated that hydroxycapsaicin was less toxic than capsaicin against …
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Turkish Journal of Biology
The sirtuin (SIRT) gene family is reported to regulate critical intracellular processes from aging to cellular metabolism and repair. SIRT3 knockout (SIRT3-/-) mice develop receptor positive mammary tumors starting at 13 months and SIRT3 expression is decreased in human breast cancer samples as well as several other diseases. It is established that carcinogenesis results from the accumulation of multiple aberrant genetic events including the activation of oncogenes and inactivation of tumor-suppressor genes. To determine the potential early genomic events that may play a role in the tumor-permissive phenotype observed in SIRT3-/- cells, we compared gene expression profile in SIRT3-/- and …
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Turkish Journal of Biology
Cyanobacteria can be used to remove nitrogenous compounds from wastewater, but a major bottleneck in the process is the separation of cyanobacterial biomass from the treated water discharge, which may cause eutrophication. The current study assessed the suitability of three periphytic cyanobacteria (Geitlerinema sp., Gloeotrichia sp., and Lyngbya sp.) isolated from shrimp ponds. These cyanobacteria were immobilized by self-adhesion to polyvinyl chloride sheets, forming mats, and were screened for their efficacy to reduce nitrogenous compounds and phosphorus. Among the three isolates, Geitlerinema sp. showed the highest reduction rate (98%) and was further tested using shrimp pond wastewater. The results showed …
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. In the current study, dental composites having 1%, 5%, and 10% (w/w) sodium pentaborate pentahydrate were prepared and analyzed for their mechanical properties, degree of monomer conversion (DC) rate, antibacterial effects against Streptococcus mutans, and biocompatibility with human dental pulp stem cells (hDPSCs). Incorporation of boron into the composites significantly decreased flexural strength and DC in a dose-dependent manner, but the value for 1% boron-containing composite still remained within acceptable levels. Compressive strength …
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Turkish Journal of Biology
No abstract provided.
Ploidy Stability Of Oryza Sativa. L Cv Ir64 Transformed With The Moth Bean P5cs Gene With Significant Tolerance Against Drought And Salinity, Arumugam Mohana Priya, Subramanian Radhesh Krishnan, Manikandan Ramesh
Ploidy Stability Of Oryza Sativa. L Cv Ir64 Transformed With The Moth Bean P5cs Gene With Significant Tolerance Against Drought And Salinity, Arumugam Mohana Priya, Subramanian Radhesh Krishnan, Manikandan Ramesh
Turkish Journal of Biology
Rice is one of the world's most important crops. Thus, it is necessary to improve it by genetic transformation. IR64 is a high-yielding and disease-resistant variety, but it is sensitive to abiotic stresses, mainly salinity and drought. Therefore, an efficient transformation system is a prerequisite for transforming this indica rice with a stress-tolerant gene, the potent osmolyte proline-coding P5CS gene of Vigna aconitifolia. Successful transgene integration was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. A Mendelian pattern of transgene inheritance was also observed in the progeny plants. The individual transformants expressed significant tolerance over wild-type control plants, …
Feasibility And Assessment Of The Phytoremediation Potential Of Duckweed For Triarylmethane Dye Degradation With The Emphasis On Some Physiological Responses And Effect Of Operational Parameters, Samaneh Torbati
Turkish Journal of Biology
Phytoremediation is a low-cost and effective technology that removes pollutants, such as dye-containing effluents, from the environment through the use of plants. In the present study, the potential of Lemna minor L. for decolorization and degradation of the triarylmethane dye malachite green was investigated. The results revealed that the decolorization ability of the plant species is as high as 88%. The effect of some operational parameters (initial dye concentration, temperature, pH, and amount (weight) of plant) on the efficiency of biological decolorization process was determined. The metabolic fate of the dye was proposed by identifying 8 intermediate compounds produced during …
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Turkish Journal of Biology
Reactive oxygen species (ROS) overproduction may severely affect cell functions and even provoke cell death. Diazoxide, an opener of ATP-sensitive potassium channels, induces pharmacological preconditioning in different types of cells. However, the target of action of diazoxide is not clear enough because this substance can activate ATP-sensitive potassium channels localized in mitochondria (mitoKATP) as well as in plasma membranes (KATP channels). It has not yet been established if diazoxide activates mitoKATP in uterus cells. Our objective was to examine the influence of diazoxide on ROS production and on the viability of rat uterus cells under oxidative stress conditions. Using an …
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Turkish Journal of Biology
The current study reveals the effects of mycorrhization on heavy metal uptake and accumulation, antioxidant potential, and essential oil composition of Origanum majorana L., grown on soil polluted with industrial Cd and Pb. Two strains of Claroideoglomus claroideum (EEZ 54 and EEZ 55) were isolated from soil that is naturally rich in metals. EEZ 35 (Funneliformis mosseae) was isolated from a place with industrial contamination. The percentage of mycorrhizal colonization with the EEZ 54 strain was higher than those of the other strains; there was no significant difference between EEZ 55 and EEZ 35. The highest value of the total …
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Turkish Journal of Biology
Arnica montana L. (Asteraceae) is an economically important herb that contains numerous valuable biologically active compounds accumulated in various parts of the plant. The effects of carbon sources (sucrose, maltose, and glucose) at different concentrations (1%, 3%, 5%, 7%, and 9%) on growth were studied and GC-MS based metabolite profiling of A. montana hairy roots was conducted. The optimal growth and biomass accumulation of transformed roots were observed on an MS nutrient medium containing 3% or 5% sucrose. GC-MS analysis of hairy roots of A. montana showed the presence of 48 compounds in polar fractions and 22 compounds in apolar …
Evaluation Of The Phytoremediation Capacity Of Lemna Minor L. In Crude Oil Spiked Cultures, Taylan Kösesakal, Vesi̇le Selma Ünlü, Oktay Külen, Abdülrezzak Memon, Bayram Yüksel
Evaluation Of The Phytoremediation Capacity Of Lemna Minor L. In Crude Oil Spiked Cultures, Taylan Kösesakal, Vesi̇le Selma Ünlü, Oktay Külen, Abdülrezzak Memon, Bayram Yüksel
Turkish Journal of Biology
Phytoremediation of freshwater contaminated with crude oil is a technology that can restore damaged freshwater areas. Lemna minor is a small vascular plant that reproduces rapidly, is sensitive to a wide variety of pollutants, and is easy to culture. This study aimed to evaluate the phytoremediation capacity of L. minor in crude oil spiked cultures. Cultivation was carried out for 7 days in a greenhouse with a natural photoperiod and in nutrient solutions containing 0.5%, 1%, 2%, and 3% crude oil. Extracts were analyzed using GC/MS and synchronous UV fluorescence spectroscopy. After a week of cultivation, the fresh weight of …
Eugenol Biologic Activity In Immunosuppressed Rat Females With Candida Albicans Genital Infection: Histocytological Changes, Romeo Teodor Cristina, Diana Obistioiu, Eugenia Dumitrescu, Ileana Nichita, Florin Muselin, Diana Brezovan, Mihai Sorin Cernea
Eugenol Biologic Activity In Immunosuppressed Rat Females With Candida Albicans Genital Infection: Histocytological Changes, Romeo Teodor Cristina, Diana Obistioiu, Eugenia Dumitrescu, Ileana Nichita, Florin Muselin, Diana Brezovan, Mihai Sorin Cernea
Turkish Journal of Biology
From our prior studies concerning eugenol's biologic activity we observed the very good in vitro antifungal efficiency of this natural compound. Those positive results generated the need to supplement the available information with a comparative in vivo animal model. In this context, our current study was proposed to ascertain and compare the effects of eugenol with nystatin with a placebo control group (saline solution) by evaluating the cytohistological alterations in immunosuppressed Wistar female rats genitally infected with Candida albicans. The observed histological alterations were most apparent in the placebo group. Candidiasis affected the liver, ovaries, kidneys, and spleen of the …
Investigation Of The In Vivo Interaction Between Ss-Lactamase And Its Inhibitor Protein, Ni̇lay Büdeyri̇ Gökgöz, Si̇may Yalaz, Naze Gül Avci, Gi̇zem Buldum, Eli̇f Özkirimli Ölmez, Berna Sariyar Akbulut
Investigation Of The In Vivo Interaction Between Ss-Lactamase And Its Inhibitor Protein, Ni̇lay Büdeyri̇ Gökgöz, Si̇may Yalaz, Naze Gül Avci, Gi̇zem Buldum, Eli̇f Özkirimli Ölmez, Berna Sariyar Akbulut
Turkish Journal of Biology
The affinity of ß-lactamase inhibitory protein (BLIP) for TEM-1 ß-lactamase has raised hopes in the challenge of protein-based inhibitor discovery for ß-lactamase-mediated antibiotic resistance. Currently, the effect of the formation of the ß-lactamase:BLIP complex in vivo in ß-lactam resistant bacteria is an open question. The scarcity of information to the extent to which BLIP can impair ß-lactamase activity inside cells has urged us to assess the in vivo efficacy of BLIP as a potent ß-lactamase inhibitor. To this end, ß-lactamase and BLIP were coexpressed in Escherichia coli. Simultaneous expression of ß-lactamase and BLIP and the formation of the TEM-1 ß-lactamase:BLIP …
In Vitro High Frequency Regeneration Through Apical Shoot Proliferation Of Hemianthus Callitrichoides `Cuba' - A Multipurpose Ornamental Aquatic Plant, Surendra Barpete, Sancar Fati̇h Özcan, Muhammad Aasim, Sebahatti̇n Özcan
In Vitro High Frequency Regeneration Through Apical Shoot Proliferation Of Hemianthus Callitrichoides `Cuba' - A Multipurpose Ornamental Aquatic Plant, Surendra Barpete, Sancar Fati̇h Özcan, Muhammad Aasim, Sebahatti̇n Özcan
Turkish Journal of Biology
Hemianthus callitrichoides 'Cuba' is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-($\gamma$,$\gamma$-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA3). Plant growth regulators significantly (P ≤ 0.001) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter (3.06 cm), area (6.12 cm), and clump weight (2.85 g) of apical shoots were achieved on MS medium …
Kremen Is Beyond A Subsidiary Co-Receptor Of Wnt Signaling: An In Silico Validation, Hemn Mohammadpour, Saeed Khalili, Zahra Sadat Hashemi
Kremen Is Beyond A Subsidiary Co-Receptor Of Wnt Signaling: An In Silico Validation, Hemn Mohammadpour, Saeed Khalili, Zahra Sadat Hashemi
Turkish Journal of Biology
Kremen-1 is a co-receptor of Wnt signaling, interacting with the Dkk-3 protein, which is an antagonist of the Wnt/ß catenin pathway. In the present study we attempted to shed some light on the possible orientations of Kremen/Dkk-3 interactions, explaining the mechanisms of Kremen and Dkk-3 functions. Employing state-of-the-art software, a Kremen model was built and subsequently refined. The quality of the final model was evaluated using RMSD calculations. Ultimately, we used the Kremen model for docking analysis between Kremen and Dkk-3 molecules. A model built by the Robetta server showed the best quality scores. Near native coordination of the final …
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Turkish Journal of Biology
Propolis is a natural, resinous, hive product with various bioactive properties. Polymorphonuclear leukocytes (PMNLs) use both oxygen-dependent (respiratory burst) and oxygen-independent mechanisms (releasing proteolytic enzymes such as elastase) to destroy infectious agents. The in vitro effects of dimethyl sulfoxide (DMSO) extract of Turkish propolis on the secretion of PMN elastase from PMNLs following respiratory burst was studied. Phorbol myristate acetate was used to stimulate respiratory burst. The Flow Cytometric CellProbe Oxidative Burst Test was used to determinate if respiratory burst occurred or not and what its severity was after a 4 h incubation with propolis extracts (0 mg/mL, 0.625 mg/mL, …
Screening And Characterization Of Biosurfactant-Producing Bacteria Isolatedfrom The Arabian Sea Coast Of Karachi, Erum Shoeb, Nuzhat Ahmed, Jameela Akhter, Uzma Badar, Khaizran Siddiqui, Faiza Ansari, Maheen Waqar, Samina Imtiaz, Nimrah Akhtar, Qurat Ul Ain Shaikh, Roanuq Baig, Saba Butt, Saema Khan, Sidra Khan, Shahid Hussain
Screening And Characterization Of Biosurfactant-Producing Bacteria Isolatedfrom The Arabian Sea Coast Of Karachi, Erum Shoeb, Nuzhat Ahmed, Jameela Akhter, Uzma Badar, Khaizran Siddiqui, Faiza Ansari, Maheen Waqar, Samina Imtiaz, Nimrah Akhtar, Qurat Ul Ain Shaikh, Roanuq Baig, Saba Butt, Saema Khan, Sidra Khan, Shahid Hussain
Turkish Journal of Biology
The aim of the present study was to investigate biosurfactant-producing culturable bacteria inhabiting the coast of the Arabian Sea at Karachi. Overall, 15 seawater samples were collected from the Arabian Sea coast of Karachi. Isolation, characterization, and screening for 89 biosurfactant-producing bacterial strains were conducted through 8 conventional screening tests. Through GSP agar plate method 22 strains were found to be Pseudomonas aeruginosa and Gram reaction revealed 70% of the isolates to be gram-negative. Furthermore, 24% of the isolates showed hemolytic activity, 44% exhibited positive results for oil-spreading test, 54% showed emulsification to at least 1 of the 3 hydrocarbons …
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Turkish Journal of Biology
In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and …
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Turkish Journal of Biology
A porcine fat mass and obesity-associated gene (pFTO) was cloned into the expression vector pET30a(+) and heterologously expressed in Escherichia coli. The effects of isopropyl ß-D-thiogalactopyranoside (IPTG) concentration and induction time on the expression of recombinant pFTO were assessed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the molecular mass of the recombinant pFTO protein was about 56 kDa, and the recombinant protein could be induced efficiently in E. coli BL21 by the addition of 0.75 mM IPTG for 4 h at 30 °C. The recombinant pFTO fusion protein was purified using Ni-IDA affinity chromatography with a yield of about 1.8 …
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Turkish Journal of Biology
The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30-90 nm), and EDX analysis. Cell viability assays were carried …
Micropropagation Of Vaccinium Myrtillus L. (Bilberry) Naturally Growingin The Turkish Flora, Mustafa Cüce, Atalay Sökmen
Micropropagation Of Vaccinium Myrtillus L. (Bilberry) Naturally Growingin The Turkish Flora, Mustafa Cüce, Atalay Sökmen
Turkish Journal of Biology
This study was designed to micropropagate Vaccinium myrtillus L., a naturally growing bilberry, in the Turkish flora. In order to determine the most effective basal medium, lateral buds were initially selected as explants, which were then individually cultured in Murashige and Skoog medium, Anderson's rhododendron medium, and McCown's woody plant medium (WPM) supplemented with 1.0 mg L-1 zeatin in combination with either 0.1 mg L-1 indole-3-butyric acid (IBA) or 0.1 mg L-1 \alpha-naphthalene acetic acid. WPM, supplemented with zeatin and IBA, was found to be the most effective basal medium tested. As shoot regeneration ability highly depends on the medium …
Salicylic Acid Alleviates Cold-Induced Photosynthesis Inhibition Andoxidative Stress In Jasminum Sambac, Han Cai, Mengying He, Kun Ma, Yonggao Huang, Yun Wang
Salicylic Acid Alleviates Cold-Induced Photosynthesis Inhibition Andoxidative Stress In Jasminum Sambac, Han Cai, Mengying He, Kun Ma, Yonggao Huang, Yun Wang
Turkish Journal of Biology
Salicylic acid (SA) is a signal molecule that mediates many biotic and abiotic stress-induced physiological responses in plants. In the current study the protective effects of SA on cold stress-caused oxidative damage and photosynthesis inhibition in jasmine plants (Jasminum sambac) were examined. Jasmine seedlings were pretreated with 100 µM SA for 3 days and then subjected to cold stress (4 °C) for 15 days. The amounts of superoxide radicals (O_2^{-}) and hydrogen peroxide (H_{2}O_{2}) significantly increased in leaves of plants exposed to cold stress and resulted in oxidative damage, indicated by an increase in malondialdehyde accumulation and membrane leakage. The …
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
Turkish Journal of Biology
Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate …
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Turkish Journal of Biology
An intracellular nitrilase (Nit1) with cyanide-degrading activity was isolated from Trichoderma harzianum VSL291, cultivated on benzonitrile as the sole carbon source. Nit1 was purified to homogeneity by ion exchange and gel filtration chromatography with a recovery of 7.15% and a fold of 22.5. The molecular weight was estimated to be 47.7 kDa and the purified enzyme was sequenced with a system of liquid chromatography and mass spectrometry (LC-MS). The enzyme consists of 436 amino acids with a predicted molecular weight of 47.088 kDa. The sequence revealed conserved domains for a nitrilase super family such as putative active and binding sites …
Energy Efficiency Of The Sunlight Harvesting And Storing System In Bacterial Photosynthesis: Comparison With Semiconductor Photovoltaic Cells, Erkin Zakhidov, Mavluda Zakhidova, Abdumutallib Kokhkharov, Abdurasul Yarbekov, Vakhobjon Kuvondikov, Sherzod Nematov, Erkin Normatov, Aziz Saparbayev
Energy Efficiency Of The Sunlight Harvesting And Storing System In Bacterial Photosynthesis: Comparison With Semiconductor Photovoltaic Cells, Erkin Zakhidov, Mavluda Zakhidova, Abdumutallib Kokhkharov, Abdurasul Yarbekov, Vakhobjon Kuvondikov, Sherzod Nematov, Erkin Normatov, Aziz Saparbayev
Turkish Journal of Biology
The light phase of photosynthesis is considered a joint operation of 2 functional pigment-protein complexes: a light harvesting antenna, absorbing sunlight in a wide spectral range, and a reaction center, utilizing the energy of absorbed light quanta in photochemical charge separation reactions. These complexes allow converting solar energy to the energy of specific biomolecules with high quantum efficiency. However, before being transferred to reaction centers, the solar energy is stored in the lowest excited state of pigment molecules of the light harvesting antenna that partially convert light quantum energy into heat. These energy losses bring a significant reduction of energy …
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Turkish Journal of Biology
This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy …
Td-Damd-Pcr Assays For Fingerprinting Of Commercial Carnations, Ayşe Gül İnce, Mehmet Karaca
Td-Damd-Pcr Assays For Fingerprinting Of Commercial Carnations, Ayşe Gül İnce, Mehmet Karaca
Turkish Journal of Biology
Dianthus L. is one of the highly valued plant species in the family Caryophyllaceae. More than 30,000 cultivars of commercial carnations have been recorded and there is a need for an effective and cheap method to reveal their genetic diversity and identify the cultivars. To the best of our knowledge this is the first report of implementation of the touchdown direct amplification of minisatellite-region DNA polymerase chain reaction (Td-DAMD-PCR) technique in the genus Dianthus. A total of 12 core minisatellite primers empirically selected from 22 primers were used in fingerprinting and phylogenetic studies of some commercial carnation cultivars. Analysis revealed …
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Turkish Journal of Biology
The aim of the current study was to evaluate lead accumulation in the ovaries, fallopian tubes, and uterus and to take note of any consequent histo-architectural changes. The experiment involved a 12-month chronic exposure of 28 Wistar female rats at sexual maturity (221 ± 0.88 g/individual) to lead acetate in drinking water. The rats were divided into 4 groups based on the level of lead exposure: E1 at 0.050 mg L^{-1}, E2 at 0.100 mg L^{-1}, E3 at 0.150 mg L^{-1} , and a control group that received tap water. Lead level evaluation was performed by atomic absorption spectrometry at …