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The Relationship Between Alpha-Defensin 1 And Gut Microbiome Composition In A Population Of Bhutanese Refugee Adults With A High Prevalence Of Type 2 Diabetes, Daisha Alana Drake

Honors Theses and Capstones

The New Hampshire Bhutanese refugee population is disproportionately affected by obesity and metabolic disorders such as type 2 diabetes. The gut microbiome is important for overall human health, with high microbial richness and diversity being associated with reduced chronic inflammation and type 2 diabetes risk. Microbial richness and diversity have been shown to decrease in United States (U.S.) immigrant populations, but have not been measured in refugee populations. One of the anti-inflammatory functions of the gut microbiome is related to the production of short-chain fatty acids (SCFAs), which regulate pro-inflammatory cytokine production. Alpha-defensin 1 is an antimicrobial peptide that is …

Neuronal Primary Cilia In Postnatal Brains & Alzheimer’S Disease Mice Eeg Patterns Under Fear Conditioning, Sierra Rose Mae Walsh

Honors Theses and Capstones

Alzheimer’s Disease (AD) is a neurodegenerative condition caused by the abnormal accumulation of amyloid β plaque. While small amounts of amyloid β plaque is to be expected with increased age, AD presents amyloid precursor protein (APP), a gene promoting inappropriate plaque formation, causing early neuronal death and tissue depreciation. APP23 transgenic mouse models contain the human mutation in the neocortical and hippocampal positions, areas revealing of plaque accumulation and memory loss. Within the hippocampal cortices, the primary cilia, which regulate higher cognition and neurodevelopment can be studied along with burst suppressions, or moments of high focal activity, to target potential …

Investigating The Role Of Bromodomain Proteins On Histone Post-Translational Modifications In Toxoplasma Gondii, Joshua Steven Berthiaume

Honors Theses and Capstones

Toxoplasma gondii is a prevalent pathogenic parasite that infects approximately 25 percent of the US population. For the parasite to successfully establish and maintain infection in its host, properly controlled regulation of gene expression is critical. One way Toxoplasma regulates gene expression is through modification of histone proteins that bind to DNA and can control gene accessibility. Acetylation is a modification that is added to histones that changes chromatin structure to enhance gene activation. Histone acetylation can also regulate gene expression by recruitment of important regulators such as bromodomain proteins (BDP). A previous study showed that loss of the bromodomain …

Effects Of Aging On The Reproductive Success Of Female Saltmarsh Sparrows (Ammospiza Caudacutus) In Maine And New Hampshire, Isabella Rose Collamati

Honors Theses and Capstones

The saltmarsh sparrow (Ammospiza caudacutus) is a ground-nesting specialist in coastal salt marshes of the Northeast. Rising sea-levels increase the loss of offspring due to tidal flooding, reducing nest success and resulting in a sharp population decline. In other avian species, age has been shown to affect nest success through altering fertility, behavior, and the number of young produced, favoring older individuals. I investigated age effects on nest success of female saltmarsh sparrows using nest monitoring data collected at four sites of a long-term demographic monitoring project: Chapman's Landing (Stratham, NH), Eldridge Marsh (Wells, ME), Popham Beach (Phippsburg, …

The Prevalence And Impact Of Dairy/Beef Crosses On Cattle Producers In New Hampshire, Eleanor J. Braun

Honors Theses and Capstones

Recent years had seen a significant rise in the use of beef semen on dairy cows in the United States. This trend was not widely understood, especially as it related to dairy farmers in New Hampshire. A survey was sent out by mail to the ninety-seven dairy farms in New Hampshire. There were thirty-seven responses to the survey. Thirty-two out of thirty-seven respondents bred beef semen to their dairy cows. Twenty-one of these respondents raised dairy x beef on their farms and twenty-six planned to continue breeding dairy x beef. The most common breeds used in dairy x beef crosses …

Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty

Honors Theses and Capstones

Nearly one out of six deaths in 2020, around ten million people, were caused by cancer, making it a leading cause of death worldwide (WHO, 2022). This major public health issue, in addition to the rise of multidrug-resistant (MDR) pathogens, provides a high demand for the discovery of new pharmaceutical drugs to be used clinically to treat these conditions. The Streptomyces genus accounts to produce 39% of all microbial metabolites currently approved for human health, indicating its potential as an important species to study for antimicrobial and anticancer agents. The long linear genome of Streptomyces contains specialized sequences known as …

The Effects Of Bacillus Velezensis Ncimb 30322 On Growth Of Chenopodium Ficifolium In Nutrient-Depleted Conditions, Ciana B. Lazu

Honors Theses and Capstones

Abiotic stressors, such as nutrient and drought stress, are a leading cause of crop yield reduction. Some bacteria and fungi are able to promote plant growth and increase stress tolerance by increasing nutrient availability through nitrogen fixation or phosphate solubilization, outcompeting pathogens, productions of volatile organic compounds (VOCs), reducing plant stress, and inducing induced systemic resistance (ISR), and thus serve as a valuable component of agricultural ecosystems to reduce dependency on chemical fertilizers. Chenopodium quinoa is a crop native to South America that has been gaining popularity in the United States because of its nutritive properties and resistance to drought …

Dog Weight, Age, And Sex Relationship With Shelter Length-Of-Availability At Pope Memorial Humane Society, Logan Lajoie

Honors Theses and Capstones

Many factors can influence length-of-availability for dogs in shelters, ranging from factors of the dogs themselves to outside factors. The external factors could be the geographical location of the shelter and its clientele, the socioeconomic status of the area, how the individual shelter uses its resources, the region's policies, and more (Scarlett, 2021, p. 18). This study evaluated length-of-availability in dogs at the Pope Memorial Humane Society, a nonprofit animal shelter that serves Strafford County, New Hampshire. This allowed for dog-specific factors to be investigated more closely. The specific factors investigated were dog weight, age, and sex compared to their …

Characterization Of Fiber Intake, The Gut-Brain-Axis, And Glycemic Control In Nh Bhutanese Refugees, Brandy Moser

Master's Theses and Capstones

The gut microbiome has a significant influence on chronic diseases, potentially through mediating inflammation and the gut-brain axis, yet underlying pathways remain unclear. Microbiomes are highly variable and can be influenced by a variety of lifestyle and environmental factors, like diet and medication use. The study of both the composition and function of the gut microbiome is warranted, yet many gaps remain particularly in our knowledge about the gut microbiome function in the context of chronic diseases. Refugee groups in the US, such as the Bhutanese refugee population in New Hampshire, are at higher risk for chronic diseases but underrepresented …

Targeting The Bcl6 Transcription Factor In Ovarian Cancer, Harrison H. Parent

Honors Theses and Capstones

Ovarian cancer is one of the most common reproductive malignancies for women in the United States, as well as one of the deadliest. Many deaths attributed to ovarian cancer, as well as cancer generally, are due to metastasis of the original tumor to different tissues throughout the body. The BCL6 transcription factor has been identified to play a key role in the metastasis of these tumors, as well as the invasion of these cancer cells through mesothelial cell layers, which contributes to ovarian cancer’s unique metastatic behavior. In an attempt to target these metastases and prevent mesothelial penetration, drugs were …

Characterization Of L-Malic Acid-Capped Cds Quantum Dots And Examination Of The Nanoncrystal's Biosensor Ability, Andrei A. Potapenko

Honors Theses and Capstones

Ligand-capped quantum dots are microscopic semiconductors commonly used in biomedical research and therapy for cell imaging and drug delivery. Despite the abundance of in vivo applications, the nature of interactions between the nanocrystals and the amino acids comprising human proteins remains insufficiently investigated. In this work, we report on the synthesis, ligand exchange, and characterization of the L-malic acid-capped CdS quantum dots via various spectroscopic techniques. We also assess the ligand-capped nanocrystals as potential probes for some amino acids. We determine that in the presence of L-cysteine, the L-malic acid-capped CdS quantum dots fluorescence signal quenches drastically, thus indicating a …

Investigating The Main Protease (Mpro) Of Sars-Cov-2 As A Potential Drug Target, Valerie Giovina Pascetta

Honors Theses and Capstones

The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 19 (COVID-19) pandemic has claimed the lives of roughly 6.2 million people worldwide as of May 2022. The virus’s main protease (M^pro ) has been identified as an attractive drug target due to the critical role it plays in the viral life cycle. The roughly 34 kDa M^pro cleaves functional viral polypeptides out of two long polyproteins at conserved cut sites, allowing them to fulfill their role in processes like transcription and replication. Here, we have studied the enzymatic activity …

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …

Finding The Best Predictors For Foot Traffic In Us Seafood Restaurants, Isabel Paige Beaulieu

Honors Theses and Capstones

COVID-19 caused state and nation-wide lockdowns, which altered human foot traffic, especially in restaurants. The seafood sector in particular suffered greatly as there was an increase in illegal fishing, it is made up of perishable goods, it is seasonal in some places, and imports and exports were slowed. Foot traffic data is useful for business owners to have to know how much to order, how many employees to schedule, etc. One issue is that the data is very expensive, hard to get, and not available until months after it is recorded. Our goal is to not only find covariates that …

The Effect Of Post-Meal Walking On 24-Hour Central Blood Pressure In Young Women With And Without Excess Adiposity, Maison P. D'Amelio, Bilal A. Chaudhry, Ciana B. Lazu

Honors Theses and Capstones

Post-meal walking (PMW) performed after breakfast, lunch, and dinner has been demonstrated to reduce blood glucose. However, no studies have examined the potential additive benefits of post-meal walking exercise on daytime central blood pressure (BP) in young women. METHODS: Thirteen physically inactive, non-hypertensive women (Age: 20±1 years; percent body fat: 28.2±13%) completed the study during the early follicular or placebo phase of their contraceptive cycle. Participants completed a control day (CON; no exercise/excess physical activity) and PMW day (3 bouts x 15 minutes of brisk walking) over five days in random order. Daytime ambulatory BP and accelerometry data (to estimate …

Impact Of Drought Stress On Oak Stomatal Size And Density At Thompson Farm, Nh, Emily Chen

Honors Theses and Capstones

Global climate change is likely to affect northeastern U.S. forests by increasing the frequency and severity of drought events. Historically, droughts rarely occurred in this region, so native tree species are not well-adapted to extreme moisture stress. In the future, the changing climate will likely cause unprecedented levels of low water availability which may have implications on future forest composition. It is hypothesized that trees can respond to these environmental changes by altering their functional traits, also referred to as phenotypic plasticity; for example, by producing leaves with fewer and smaller stomata.

To determine the capacity to which oak trees …

Factor Fiction? Identifying A Putative Toxoplasma Gondii Transcriptional Complex, Julia M. Paquette

Honors Theses and Capstones

Toxoplasma gondii is a highly prevalent protozoan parasite that is estimated to infect 30-50% of the global population, though there is no treatment for chronic infection and current treatments for acute infection may have serious side effects. Transcription is a tightly regulated process in T. gondii, allowing the parasite to successfully invade and replicate within host cells, and it is thus a promising avenue to study gene regulation and to investigate possible novel therapeutics. In our lab’s previous research, a TFIID-like complex was identified in T. gondii and found to be associated with the parasite specific bromodomain protein BDP3. …

Engineering Thermostable Regulators For Inducible Gene Expression In Thermophiles, Connor M. Joyce

Honors Theses and Capstones

Thermophilic bacteria have attracted research interest due to their ability to grow at high temperatures ranging from 45 °C to 75 °C with some extreme thermophiles able to survive nearly boiling temperatures. They are valuable sources of thermostable biocatalysts and many have great potential as industrial hosts for biofuel production because fermentation at high temperatures has advantages such as reduced processing costs and lower risk of contamination compared with mesophilic bacteria. For example, some Geobacillus species has been shown to have high solvent tolerance, making them good candidates as host for alcohol production. To develop them as industrial hosts, large …

A Survey Of Opportunistic Fungi In The Portsmouth, Nh Region, Chloe E. Richard

Honors Theses and Capstones

Opportunistic mycoses are caused by ubiquitous fungi that we encounter daily. This study identified the most common opportunistic fungi in a local area during the current winter season. A survey of fungi was conducted in the Portsmouth, New Hampshire region by taking environmental samples from both indoor and outdoor locations. The samples were then cultured and identified using colony morphology, microscopic characteristics, and biochemical testing. Despite the cold weather, the results showed that fungal species are still prevalent outdoors, with the most variation in single samples coming from areas with exposed soil and decaying vegetation, while less fungal presence was …

Effects Of The Air Quality In Equine Stable Environments On The Respiratory Health And Allergy Response Of Human Personnel: A Review, Maeve Hannah Perrin

Honors Theses and Capstones

Many studies have evaluated the impact of poor stable air quality on equine respiratory health and respiratory illness in horses. Many factors contribute to poor air quality conditions in the equine stable environment, including ventilation rates, humidity levels, presence of noxious gases, rate of fungal spore production, and level of airborne organic and inorganic dust particles. While the implications of poor air quality for equine health are well-documented, far less is known about how air quality impacts human stable personnel. This literature review seeks to evaluate the implications of poor equine stable air quality on the respiratory health and allergic …

Mimicking The Effect Of Prolactin On Stat3/Stat5 Activity In Breast Cancer, Jennifer N. Barbuto, Sarah R. Walker

Honors Theses and Capstones

Signal transducers and activators of transcription (STAT) 3 and 5 are commonly constitutively activated in breast cancer. STAT5 can outcompete STAT3 and reduce cell proliferation and metastasis. STAT5 activation is stimulated by prolactin, a natural hormone that can be harmful at high levels. The aim of this study is to identify some possible previously developed drugs that mimic the effect of prolactin and STAT5 without the added risk in MDA-MB231 breast cancer cells. Using the CLUE database query app and STAT5 up- and downregulation signatures, three drugs (X, K, and M) were chosen based on their similarity in signatures to …

Roles Of Non-Frankia Bacteria In Root Nodule Formation And Function In Alnus Sp., Kelsey Christine Mercurio

Honors Theses and Capstones

Plant roots are home to a wide variety of beneficial microbes; understanding and optimizing plant-microbe interactions may be critical to enhance global food security in a sustainable, equitable way. With the help of their nitrogen-fixing bacterial partner, Frankia, actinorhizal plants form symbiotic root nodules and play important roles in agroforestry and land reclamation. However, Frankia does not live alone in nodules, and the other microbial residents may contribute to nodule formation and function. We collected root nodules from alder trees (Alnus sp.) in 2018 and 2019, then isolated DNA and individual bacterial strains to characterize the nodule microbial …

Expanding On The Involvement Of Primary Cilia In Neurological Function Through Observing Eeg/Emg Changes In Different Genotypic Mice, Gabriella M. Palermo

Honors Theses and Capstones

Primary cilia have been known to regulate neurological functions, as primary cilia exist on astrocytes and neurons in the mature brain. More is being discovered about primary cilia and their role in the vertebrate nervous system; however, little is known about their functions and molecular pathways in the mature brain. There also lacks methods of diagnosis for primary cilia-related defects and diseases. The purpose of this project was to provide more information on the involvement of cilia in the neurological functions and offer a potential means for diagnosis of cilia-related disorders by comparing the EEG and EMG signals of wildtype …

Sars-Cov-2: An Investigation On Mutagenicity And Its Effects On Infectivity And Mortality, Tyler Elliott Silverwood

Honors Theses and Capstones

SARS-CoV-2, the etiological agent of the COVID-19 pandemic, has rapidly become a worldwide public health concern. Classified as a betacoronavirus, it is the third human coronavirus (HCoV) to emerge in the 21st century that causes severe disease, alongside SARS-CoV and MERS-CoV. The genome consists of open reading frames encoding accessory proteins and four structural proteins, including the spike protein which is a key determinant of host cell tropism. Mutations within the genome, particularly the spike gene, have been linked in-vitro to increased binding affinity to the human receptor angiotensin-converting enzyme 2 (hACE2), increased fitness in human hosts, and immune evasion. …

Occurrence Of Pulmonary Arteritis With Lungworm Infections In Gray Seals From New England, 2013-2020, Jillian R. Broadhurst, Inga Sidor Dvm, Ms, Dacvp

Honors Theses and Capstones

Gray seals (Halichoerus grypus) are a common pinniped species in New England coastal waters. Parasitic infection is an important cause of mortality for this species, and lungworm species are frequently implicated in cases of respiratory infection, especially in pups and weanling seals. The most common of these is Otostrongylus circumlitus, a large nematode found in the bronchi and bronchioles of affected seals. The full life cycle is unknown but its presence in lungs and sometimes pulmonary arteries suggests that migration via arterial circulation occurs. Signs and microscopic findings are widely variable; our cases included dyspnea, bloody mucoid …

Impacts Of Inappropriate Human Possession Of Wildlife On The Animal’S Well-Being, Anika Frink

Honors Theses and Capstones

Wildlife rehabilitation is the temporary intervention by humans on sick or injured wild animals, who would not otherwise survive, with the goal of release. Individual states have their own guidelines for how to become a licensed rehabilitator with extensive standards in place for how to keep such wildlife. The standards help to keep the animals wild for their eventual release and to avoid side effects of Inappropriate Human Possession or IHP. IHP is when an unlicensed person attempts to rehabilitate a wild animal or to keep it as a pet. This study examines the effects of IHP on wildlife patients …

The Evolution And Development Of Chiropteran Flight, Emmaline Willis

Honors Theses and Capstones

No abstract provided.

Tick-Borne Infections In New Hampshire: An Evaluation Of The Diagnostic Process In A Local Patient Population, Katherine Anderson

Honors Theses and Capstones

Overall, approximately 95 percent of reported cases of vector-borne disease were associated with ticks, making these the most medically important group of arthropods in the United States.¹ Despite the prevalence of tick-borne infections, the process for the diagnosis of this condition is not well studied. This study aims to analyze data from a pool of 100 patients who underwent testing for tick-borne disease in the same institution in Dover, New Hampshire during the most recent peak tick season of 2019. Information utilized in this study included: patient age, sex, location of testing (inpatient versus outpatient), diagnostic testing methods used …

“C1orf150”: A Novel Regulator Of Jak2 Kinase, And Candidate Tumor Suppressor In Human Blood Cell Progenitors, Tyler M. Wade

Honors Theses and Capstones

Erythropoietin (EPO) is an essential growth factor for red blood cell (RBC) production. In response to anemia, hypoxia-sensing cells in the kidney express and release EPO. EPO then acts in bone marrow to drive RBC formation from erythroid progenitors. Upon binding to its cell surface receptor, EPO triggers a JAK2 kinase signaling cascade for progenitor cell growth. Our lab has discovered a novel regulator of JAK2, “C1ORF150” (“150”). “150” is conserved in H sapiens and primates and is a new orthologue of the B-cell receptor adaptor protein and tumor marker, HGAL. Using a shRNA knockdown approach, I investigated the actions …

Do Trellis Systems Modify Grape's Health Beneficial Properties?, Molly Hanlon

Honors Theses and Capstones

No abstract provided.