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Biosecurity Risk Assessment Of The Fairchild Dairy Nutrition Research Center, Margaret Lynch
Biosecurity Risk Assessment Of The Fairchild Dairy Nutrition Research Center, Margaret Lynch
Honors Theses and Capstones
No abstract provided.
Variations In Carotenoids In Plasma From Jersey Cows At An Organic Dairy Compared To A Conventional Dairy Over Time, Jillian M. Smith
Variations In Carotenoids In Plasma From Jersey Cows At An Organic Dairy Compared To A Conventional Dairy Over Time, Jillian M. Smith
Honors Theses and Capstones
Interest in organic milk is increasing. By USDA Certified Organic standards, animals must be fed on pasture for a minimum of 120 days. Research indicates that various factors such as diet impact the nutritional quality and composition of milk. The objective of this study was to understand how feeding practices affected bovine plasma carotenoid concentrations over time. Plasma samples were collected from 9 cows fed on pasture at an organic dairy and from 9 cows fed total mixed ration (TMR) at a conventional dairy. Components were extracted from the plasma and separated via High Performance Liquid Chromatography. Results showed that …
One Can’T Stand On Its Own: Are Non-Luminescence Traits Necessary For V. Fischeri Colonization Of E. Scolopes?, Feier Liu
Honors Theses and Capstones
Vibrio fischeri and Euprymna scolopes squid establish mutualistic symbiosis and select for each other in the natural environment. V. fischeri provides bioluminescent camouflage for E. scolopes while E. scolopes provides nutrients for V. fischeri. The most intriguing aspect of this relationship is that E. scolopes is highly selective and only allows sustained colonization by luminous, but not dark V. fischeri. Luminescence is the key symbiosis trait; however, other bacterial factors may also allow squid recognition. We hypothesized that there are luminescence linked traits that contribute to colonization. V. fischeri with luminescence variation was isolated and tested for oxidative resistance, …
The Effects Of Cytokinin On The Transcriptional Regulation Of Pin Expression In Arabidopsis Thaliana, Elizabeth Burgess
The Effects Of Cytokinin On The Transcriptional Regulation Of Pin Expression In Arabidopsis Thaliana, Elizabeth Burgess
Honors Theses and Capstones
The processes of cell division and differentiation are critical to the development of any multicellular organism. During the formation of plant roots these processes take place at a region of the root tip called the meristem. Cytokinin and auxin are two plant growth hormones that influence this process. Although these two growth hormones are both necessary they also appear in many ways to have an antagonistic relationship. As meristematic root cells undergo differentiation they cease dividing. It has been proposed that the size of the root meristem and thus the overall rate of root growth are determined by the balance …
Replication Protein A (Rpa70c) Negatively Regulates Ribonucleotide Reductase (Rnr) In The Model Plant Arabidopsis Thaliana, Stacy Wong
Honors Theses and Capstones
Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays an important role in cellular responses to DNA damage. For example, RPA can activate the cell-cycle checkpoint protein ATR in the presence of persisting DNA damage. The model plant Arabidopsis thaliana has 5 functional homologous RPA70 genes which may play specific roles in response to DNA damaging agents. One chemical that causes DNA damage in Arabidopsis is Hydroxyurea (HU), which blocks DNA replication by inhibiting activity in Ribonucleotide Reductase (RNR), an enzyme responsible for the production of free deoxyribonucleotides (dNTPs). In studies of Arabidopsis mutants, atr plants, but …
Genetic Engineering Of Dunaliella: Potential For Improved Biofuel Production, Alison Wirshing
Genetic Engineering Of Dunaliella: Potential For Improved Biofuel Production, Alison Wirshing
Honors Theses and Capstones
Biodiesel produced from algal lipids is a promising source of renewable energy. Reasons for using algae, as opposed to vascular plants, are numerous. Oleaginous algae do not require fertile land or fresh water to grow, and therefore do not compete with food crop resources. Algae have short generation times and higher growth rates than larger vascular plants allowing for synthesis and accumulation of large quantities of neutral lipids, about 20-50% of the dry cell weight, in a short period of time. The efficiency of biodiesel production from oleaginous algae could be improved by using transgenic (genetically engineered) algae. The goal …
The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz
The Effects Of Dependent Infants On The Social Behavior Of Mantled Howler Monkeys (Alouatta Palliate), Keziah Katz
Honors Theses and Capstones
Six weeks of observation on eight groups of wild mantled howler monkeys revealed that the presence of a dependent infant alters the frequency of social interactions between a female with an infant and other adult monkeys. Males preferred to spend time with females without infants but still spent more time with females with infants than with males. Females without infants spent significantly less time with females with infants than with males or other females without infants and females with infants decreased their frequency of social interaction overall without preference for males, females or other females with infants.
Transformation Of Tobacco With The Ba5 Cement Protein Gene From Balanus Amphitrite, Matt Marquis
Transformation Of Tobacco With The Ba5 Cement Protein Gene From Balanus Amphitrite, Matt Marquis
Honors Theses and Capstones
Expressing barnacle cement proteins genes such as the BA5 gene in plants may enable individual study and analysis. This technique is effective since barnacle cement is difficult to work with as a whole in the lab setting. The BA5 gene extracted from Balanus amphitrite is transferred to tobacco leaf tissue using Agrobacterium tumefaciens.
Bigger Bodies, Bigger Bruises: How Men’S Collegiate Hockey Player Sizes Affect Injury Rates 1989-2004, Kimberly Deane
Bigger Bodies, Bigger Bruises: How Men’S Collegiate Hockey Player Sizes Affect Injury Rates 1989-2004, Kimberly Deane
Honors Theses and Capstones
This study looks at men's collegiate hockey player sizes from 1989-2004 and correlates that data with the growth in injury rates seen within the same time period. Results show that player size (in terms of weight) has a statistically significant effect on player injury rates.
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Defeating Cytoplasmic Sequestration Of P53 In Human Breast Cancer Cells; Is Mortalin Involved?, Sarah Yunes
Honors Theses and Capstones
Cytoplasmic sequestration of p53, possibly caused by p53 interacting with mortalin, can prevent p53 from functioning in DNA repair and apoptosis, causing aberrant growth. This project treated SKBR3 breast cancer cells with MKT-077, a dye that is a competitive binder to mortalin to see if it would result in the release of p53 from the cytoplasm and restoration of p53 function. Treatment resulted in partial translocation of a protein suspected to be p53 to the nucleus and apoptosis initiated at the mitochondria.
A Messenger Molecule Governs Interdependency In An Evolved Pseudomonas Aeruginosa Biofilm Community, Thomas Macelliott Johnson
A Messenger Molecule Governs Interdependency In An Evolved Pseudomonas Aeruginosa Biofilm Community, Thomas Macelliott Johnson
Honors Theses and Capstones
Biofilm populations are known to harbor great diversity, but the importance of this diversity is not fully understood. A likely contributor to this variation is the second messenger molecule cyclic-di-GMP: low levels associate with a planktonic lifestyle while high levels favor biofilm formation. In ongoing studies of an evolving biofilm population of Pseudomonas aeruginosa (PA), we observed extensive ecological diversification that may relate to this lifestyle switch. In PA, the gene bifA encodes a phosphodiesterase that is known to degrade cyclic-di-GMP and reduce biofilm. This gene was cloned onto a plasmid under control of an inducible promoter and the plasmid …