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Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan Jan 2011

Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan

Turkish Journal of Biology

The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …


Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak Jan 2011

Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak

Turkish Journal of Biology

The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …


Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian Jan 2011

Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian

Turkish Journal of Biology

In recent years, emphasis has been placed on the use of natural materials in the control and treatment of various infections, as some chemically synthesized drugs have undesirable side effects. In this study, the ethanol extract of Iranian walnut leaves was examined for antibacterial activities on Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, and Actinomyces viscosus using the microdilution method. Total phenols, flavonoids, and flavonols were also determined colorimetrically. The minimum inhibitory concentrations (MIC) for ethanolic extract ranged between 15.6 and 187.5 mg/mL and minimum bactericidal concentrations (MBC) ranged between 31.25 and 250 mg/mL. Total phenols were 410 ± 14.43 mg/g …


Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇ Jan 2011

Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇

Turkish Journal of Biology

The volatile compounds of 13 Turkish pine (Pinus brutia Ten.) honey samples were characterized by solid phase microextraction (SPME) analysis, followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 42 volatile compounds were identified, but volatile compounds such as nonana1, benzene, 4-hexen-3-ol, alpha-pinene, and 2-heptanone were recognized to be specific floral origin markers of the pine honey. The SPME extraction method was proposed as an alternative way to carry out pollen analysis for floral source detection, especially for products such as pine honey, by characterization of honeydew elements.


The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz Jan 2011

The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz

Turkish Journal of Biology

Oylum Höyük falls within the borders of the province of Kilis, located in the southern part of Turkey. It is an important Bronze Age settlement dating back to 5500 BC. In this study, the sex of 36 individuals found in Oylum Höyük was examined using morphological and genetic methods. In the morphological method, sex was identified in 14 female and 13 male individuals. The remaining 8 individuals could not be identified due to the poor preservation of their skeletal remains. Genetic sex was determined with the amplification of sex-specific X, Y centromeric alphoid repeats and ZFX-ZYF gene regions of DNA, …


Studies On The Effect Of Ph And Carbon Sources On Enzyme Activities Of Some Pectinolytic Bacteria Isolated From Jute Retting Water, Biswapriya Das, Ashis Chakraborty, Sagarmoy Ghosh, Kalyan Chakrabarti Jan 2011

Studies On The Effect Of Ph And Carbon Sources On Enzyme Activities Of Some Pectinolytic Bacteria Isolated From Jute Retting Water, Biswapriya Das, Ashis Chakraborty, Sagarmoy Ghosh, Kalyan Chakrabarti

Turkish Journal of Biology

The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase (PG), pectin lyase (PNL), xylanase, and cellulase, of some pectinolytic bacteria were studied. The pectinolytic bacterial strains were isolated from the jute retting water bodies of 2 important jute growing areas of North 24 Parganas, West Bengal, India. The highest PG (12.45 to 41.0 IU/g cell wet weight) and PNL (22.55 to 75.7 U/mL) activities occurred at the alkaline pH of 8.0 and 8.5, respectively. Maximum xylanase activity (0.01 to 0.735 \mumol/mL/min) occurred at pH …


A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan Jan 2011

A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan

Turkish Journal of Biology

The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential …


Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel Jan 2011

Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel

Turkish Journal of Biology

This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 \muM \mu-naphthalene acetic acid (NAA) and 2.22 \muM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 \muM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 \muM). The most …


Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary Jan 2011

Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary

Turkish Journal of Biology

A secondary metabolite, 2^'-O-ethylmurrangatin (1), was isolated from the leaves of a medicinal plant, Murraya paniculata. The structure of compound 1 was identified with the help of spectroscopic techniques. Compound 1 was screened for its antioxidant, lipoxygenase, prolyl endopeptidase, and respiratory burst inhibitory activities. 2^'-O-Ethylmurrangatin (1) was found to have significant activity against lipoxygenase enzyme and moderate respiratory burst inhibitory activity, but no activity against either DPPH radical scavenging assay or prolyl endopeptidase enzyme.


Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak Jan 2011

Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak

Turkish Journal of Biology

Ethanolic (EEAA) and sesquiterpene lactone (SEAA) extracts of the aerial parts of Anthemis aciphylla var. aciphylla Boiss. (Asteraceae) were evaluated for anti-inflammatory activity on carrageenin-induced paw edema (acute model) and cotton pellet-induced granuloma (chronic model) in rats. In carrageenin-induced paw edema, EEAA and SEAA at intraperitoneal doses of 50, 100, and 200 mg/kg dose-dependently inhibited the paw edema. In cotton pellet-induced granuloma, the oral administration of EEAA and SEAA at 50, 100, and 200 mg/kg dosages was also found to significantly inhibit granuloma tissue formation.


Molecular Cloning, Characterization, And Homologous Expression Of An Endochitinase Gene From Bacillus Thuringiensis Serovar Morrisoni, Sezer Okay, Gülay Özcengi̇z Jan 2011

Molecular Cloning, Characterization, And Homologous Expression Of An Endochitinase Gene From Bacillus Thuringiensis Serovar Morrisoni, Sezer Okay, Gülay Özcengi̇z

Turkish Journal of Biology

The endochitinase gene (chi3023) of Bacillus thuringiensis (Bt) serovar morrisoni strain 3023 was amplified via polymerase chain reaction (PCR) and cloned in Escherichia coli. The ORF of chi3023 (GenBank Accession Number: DQ993175) consists of 2031 nucleotides encoding a 676-residue protein with a calculated molecular mass of 74.5 kDa and a pI value of 6.0. The amino acid sequence of Chi3023 was compared with previously sequenced Bt chitinases and the phylogenetic relationships among them were determined. The deduced N-terminal 34 amino acids of the premature Chi3023 exhibited a typical signal peptide. The E. coli-Bt shuttle vector pHT315 was used for homologous …


Characterization Of Lactococcin Bz Produced By Lactococcus Lactis Subsp. Lactis Bz Isolated From Boza, Di̇dem Şahi̇ngi̇l, Hi̇lal İşleroğlu, Zeli̇ha Yildirim, Mustafa Akçeli̇k, Meti̇n Yildirim Jan 2011

Characterization Of Lactococcin Bz Produced By Lactococcus Lactis Subsp. Lactis Bz Isolated From Boza, Di̇dem Şahi̇ngi̇l, Hi̇lal İşleroğlu, Zeli̇ha Yildirim, Mustafa Akçeli̇k, Meti̇n Yildirim

Turkish Journal of Biology

A bacterium isolated from boza produced in Turkey was identified, and the physico-chemical and microbiological properties of its inhibitory compound were characterized. The isolate was identified as Lactococcus lactis subsp. lactis, based on morphology, physiology, carbohydrate fermentation, the fatty acid profile, and 16S rDNA gene sequence homology. The antimicrobial compound produced by the microorganism, lactococcin BZ, was sensitive to papain, pepsin, trypsin, and \beta-mercaptoethanol, but was resistant to catalase, amylase, lipase, organic solvents (methanol, chloroform, etc.), detergents (SDS, urea, Tween-80, Triton X-100), and EDTA. Lactococcin BZ was active against several gram-positive and gram-negative foodborne pathogens and food spoilage bacteria. Lactococcin …


The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel Jan 2011

The Effects Of The Composition Of Growth Medium And Fermentation Conditions On The Production Of Lipase By R. Delemar, Ünsal Açikel, Mehtap Erşan, Yeşi̇m Sağ Açikel

Turkish Journal of Biology

Lipases (triacylglycerol hydrolases) are hydrolytic enzymes that can catalyze the hydrolysis of the ester bond of long-chain acylglycerols at the oil-water interface. The present study investigated the effects of inducers, surface-active materials, activators, and inhibitors in the fermentation medium on lipase activity in Rhizopus delemar. In the presence of certain commercial oils and tributyrin as an inducer, lipase activity decreased in the order of sunflower oil > soybean oil > hazelnut oil > corn oil > tributyrin > olive oil. In addition, the effects of the stirring and aeration rates on lipase activity were investigated. To investigate the effect of surface-active materials on lipase activity …


Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal Jan 2011

Biochemical Factors Involved In The Resistance Of Some Smoked Tropical Fish Samples To Insect Attack, Akinola John Akintola, Oluwatoyin Lawal

Turkish Journal of Biology

The role of mineral elements (ppm), protein and oil content (%), and saponification and free fatty acid values in contributing to resistance against insect infestation was investigated in 4 fish species: Clarias gariepinus, Parachuma obscura, Hydrocynus sp., and Malapterus electricus. Smoked fish samples were purchased from a local market in Ilorin, Kwara State, Nigeria, packaged, and maintained at 4 ^°C for 7 days. They were later brought out to acclimatise, and then observed for 4 months for possible natural insect infestation. Next, crushed samples of each species were analysed using conventional chemical analysis in order to assess the role of …


Chromosome Aberrations Induced By Curcumin And Aloin In Allium Cepa L. Root Meristem Cells, Loganathan Palanikumar, Irulappan Ragunathan, Natarajan Panneerselvam Jan 2011

Chromosome Aberrations Induced By Curcumin And Aloin In Allium Cepa L. Root Meristem Cells, Loganathan Palanikumar, Irulappan Ragunathan, Natarajan Panneerselvam

Turkish Journal of Biology

To date, a variety of plant compounds have been studied extensively with regard to anti-mutagenic, anti-carcinogenic, and anti-genotoxic activity. Nevertheless, very few of them have been studied in terms of clastogenicity. In the present study the clastogenic activity of curcumin (the active component of turmeric) and aloin (the active component of aloe) was evaluated at doses of 5, 10, 20, 30, 50, and 100 µg/mL for 4, 8, and 12 h. Both curcumin and aloin had significant dose- and time-dependent clastogenic effects on the test plant system (Allium cepa L.). Compared to curcumin, aloin was more clastogenic. Clastogenic activity was …


The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen Jan 2011

The Toxicity Of A Synthetic Industrial Detergent And A Corrosion Inhibitor To Brackish Water Fish (Tilapia Guineensis), Dori̇s Fouwe Ogeleka, Lawrence Ikechukwu Ezemonye, Feli̇x E. Okieimen

Turkish Journal of Biology

The lethal effects resulting from incessant release of toxic surfactant-containing industrial chemicals into the Niger Delta Ecological Zone of Nigeria was studied using a laboratory toxicity test. The study employed the Organisation for Economic Development and Cooperation (OECD) 203 protocol. The test organism (Tilapia guineensis [fish]) was obtained from a brackish water environment and exposed to Neatex (industrial detergent) and Norust CR 486 (corrosion inhibitor) at concentrations of 6.25, 12.5, 25, 50, and 100 mg/L for 96 h. The ecological endpoint considered was mortality at 96 h, and the results obtained indicate that concentration and duration of exposure influenced the …


Antioxidant And Antimicrobial Activity Of Turkish Endemic Sonchus Erzincanicus Extracts, Ahmet Mavi̇, Ni̇met Yi̇ği̇t, Demet Yi̇ği̇t, Ali̇ Kandemi̇r Jan 2011

Antioxidant And Antimicrobial Activity Of Turkish Endemic Sonchus Erzincanicus Extracts, Ahmet Mavi̇, Ni̇met Yi̇ği̇t, Demet Yi̇ği̇t, Ali̇ Kandemi̇r

Turkish Journal of Biology

Sonchus erzincanicus (Asteraceae) is a plant species that is endemic to Turkey and grows in Erzincan. Lipid peroxidation inhibition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and total phenolic content of the methanol and water extracts of the plant were determined. Antimicrobial activity of the extracts was also tested against 107 clinical isolates of human pathogenic microorganism strains belonging to Enterobacter aerogenes, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans using the disc-diffusion method, and the minimal inhibition concentration (MIC) value of each active extract was determined. The extracts were observed to have antioxidant potential, although no better than …


Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er Jan 2011

Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er

Turkish Journal of Biology

Venom from the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects towards hosts. Here, we further investigate if parasitism or envenomation by P. turionellae alters total protein of its host Galleria mellonella L. (Lepidoptera: Pyralidae). Various venom concentrations representing doses previously determined to yield host responses yet fall below the calculated LD99 were used for pupae and larvae. Parasitization was only assayed for host pupa since P. turionellae females normally parasitize host prepupae and pupae in nature. Hemolymph total protein concentration remained relatively steady at all doses …


Biochemical Response Of Olea Europaea Cv. Gemlik To Short-Term Salt Stress, Mehmet Ali̇ Demi̇ral, Deni̇z Aktaş Uygun, Murat Uygun, Erkan Kasirğa, Ari̇fe Alev Karagözler Jan 2011

Biochemical Response Of Olea Europaea Cv. Gemlik To Short-Term Salt Stress, Mehmet Ali̇ Demi̇ral, Deni̇z Aktaş Uygun, Murat Uygun, Erkan Kasirğa, Ari̇fe Alev Karagözler

Turkish Journal of Biology

One-year-old olive (Olea europaea L. cv. Gemlik) seedlings were exposed to increasing levels of salinity in pot culture. The experiment was laid out in a randomized block design with 6 replicates. Dry matter (DM) percentage, sodium (Na) and chloride (Cl) concentrations, 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, reducing power (RP), total phenolic content (TPC), and proline (PRO) content were determined in the leaves of the plants. The results showed that the olive plant used physiological and biochemical mechanisms consecutively to alleviate the effects of salt stress. The type of mechanism used was related to the severity of the salt stress. Salinity-induced …


In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken Jan 2011

In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken

Turkish Journal of Biology

Forty-five fungal isolates were obtained from sclerotia of Rhizoctonia solani on potato tubers in Erzurum, Turkey. The interaction between fungal isolates and R. solani was studied in dual culture technique. Some fungal isolates affected R. solani by antibiosis and/or parasitism. Results of the antagonism tests showed that Acremonium sp., Gliocladium viride, Paecilomyces marquandii, Paecilomyces sulphurellus, Penicillium camemberti, Penicillium expansum, Penicillium frequentans (ME-50), Penicillium nigricans, Penicillium olsonii, Penicillium phialosporum, Sporothrix sp. (MCY-4), Sporothrix schenckii, and Verticillium dahliae isolates produced an inhibition zone in front of the R. solani colony to a varying degree. Trichoderma harzianum isolates were able to overgrow the …


Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n Jan 2011

Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n

Turkish Journal of Biology

In the present study, a total of 290 actinomycetes isolates were obtained from 19 karstic caves in Turkey. Out of these, 131 were chosen for numerical classification studies according to their morphology and pigmentation properties. Representative isolates were compared with the reference Streptomyces strains based on their various taxonomic characteristics such as biochemical, physiological, nutritional, and morphological, totalling 134 unit characters. The data were evaluated using simple matching coefficients (S_{SM}), and clustering achieved using the Complete algorithm by X-Taxon and NTSys-pc programs. The test strains were assigned to 9 major (5-11 strains), 18 minor (2-4 strains), and 31 single membered …


Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz Jan 2011

Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz

Turkish Journal of Biology

A series of studies were carried out to determine the succession and phytoplankton community of the Melen River in the Western Black Sea region of Turkey. The study was conducted at 6 stations at monthly intervals between May 2003 and April 2004. It was observed that the abundance and biovolume of phytoplankton were low in winter, though higher values were recorded in late spring and summer. Among the values noted, the spring peak was 534.38 × 10^5 ind. L^{-1} and 7384.8 mm^3 L^{-1} belonging to Peridinium sp. The most common diatom, Cyclostephanos dubius, was favoured by low discharge at stations …


Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz Jan 2011

Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz

Turkish Journal of Biology

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 …


Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh Jan 2011

Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh

Turkish Journal of Biology

Out of 70 fungal isolates recovered from soil, wall paints (Latex), and pieces of plastic debris from different habitats in Jordan and plastic shields of street light posts on campus, 35 isolates showed varied potential to degrade polyester-polyurethane (PS-PUR). Six of these isolates (Fusarium solani, Alternaria solani, Spicaria spp., Aspergillus fumigatus, Aspergillus terreus, and Aspergillus flavus) were selected on the basis of their growth rates on basal salt media amended with PS-PUR as the sole source of carbon. Isolates were further evaluated utilizing 3 different methods: 1) direct plating, 2) clear zone in a 2-layered agar media, and 3) liquid …


Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi Jan 2011

Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi

Turkish Journal of Biology

Biological control of soilborne plant pathogens is a possible alternative to the use of chemical pesticides, which are harmful to the environment. Under greenhouse and field conditions, several isolated strains of the fungus Trichoderma have been found to be effective biocontrol agents of various soilborne plant pathogenic fungi. The use of phosphate solubilizing microorganisms (PSMs) has a considerable synergistic effect on the growth and development of crop plants. In the present study, T. harzianum was isolated from 10 different agro-climatic zones of Karnataka and tested for the potential of antagonistic activity against the plant pathogen Xanthomonas and the ability of …


C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz Jan 2011

C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz

Turkish Journal of Biology

In the present study, the 2n = 54 chromosomal race of blind mole rats, Nannospalax xanthodon superspecies, from Kırıkkale Province in Turkey was investigated. Conventional chromosome staining, Ag-NOR (Nucleolus Organizer Region) staining, and C-banding analysis were carried out on specimens of mole rats. The karyotype including 3 metacentric pairs (nos. 1-3), 3 submetacentric pairs (nos. 4-6), 3 subtelocentric pairs (nos. 7-9), and 17 acrocentric pairs (nos. 10-26) of autosomes (NFa = 70). C-heterochromatin regions were found in the centromeric and pericentromeric region and the short arms of some bi-armed autosomal pairs, and C-heterochromatin was localized in pericentromeric areas of a …


The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş Jan 2011

The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş

Turkish Journal of Biology

The suprachiasmatic nucleus (SCN) generates and controls the circadian rhythms in mammals including the rhythm of locomotor activity. Leptin is a hormone secreted by adipose tissue that informs the brain about the fat stores. SCN neurons express leptin receptors. Here we investigated the effects of 3 different leptin administrations on the locomotor activity of the Syrian hamsters maintained in constant darkness. Animals were intraperitoneally (ip) injected (4 µg/kg), subcutaneously (sc) infused (4 µg/kg), or intra-SCN infused (0.4 µg/kg) with leptin for 3 days at circadian time 10 whereas the controls received saline (0.9% NaCl) at the same time in order …