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Prevalence Of Thermoactinomyces Thalpophilus And T. Sacchari Strains With Biotechnological Potential At Hot Springs And Soils From West Anatolia In Turkey, Ataç Uzel, Eli̇f Esi̇n Hameş Kocabaş, Erdal Bedi̇r
Prevalence Of Thermoactinomyces Thalpophilus And T. Sacchari Strains With Biotechnological Potential At Hot Springs And Soils From West Anatolia In Turkey, Ataç Uzel, Eli̇f Esi̇n Hameş Kocabaş, Erdal Bedi̇r
Turkish Journal of Biology
Hot spring sediment and soil samples from West Anatolia in Turkey were investigated for the occurrence of thermophilic Actinomycetes. Isolation was done using Actinomycetes isolation agar (AIA), starch casein agar and glycerol yeast extract agar at 55 °C. Extracellular protease activity of the isolates were screened by using AIA plus 1% casein according to the hydrolysis zones surrounding the colonies. Antimicrobial activities of the isolates were also screened by using streaked plate method against a panel of test bacteria. Identification of the isolates was made by cultural, physiological characteristics and 16S rDNA sequence similarity. Sixty-seven thermophilic Actinomycetes isolates were classified …
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus (Pprv) Isolated In Iran Based On Partial Sequence Data From The Fusion (F) Protein Gene, Majid Esmaelizad, Saber Jelokhani-Niaraki, Rohani Kargar-Moakhar
Turkish Journal of Biology
In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates …