Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- Antioxidant (6)
- Antioxidant activity (4)
- Antimicrobial activity (3)
- Turkey (3)
- Antibacterial (2)
-
- Antifungal (2)
- Arnebia densiflora (2)
- Biofilms (2)
- Biology (2)
- Boza (2)
- Distribution (2)
- Extract (2)
- Fish (2)
- Flavonoids (2)
- Gene cloning (2)
- Insecticidal activity (2)
- Lander College of Arts and Sciences (2)
- Microbial control (2)
- Mitotic index (2)
- Numerical taxonomy (2)
- Parasitism (2)
- Persistence (2)
- Phylogenetic analysis (2)
- Resistance (2)
- Science (2)
- Stability (2)
- Streptomyces (2)
- Student research (2)
- Total phenolics (2)
- Touro College (2)
Articles 61 - 90 of 120
Full-Text Articles in Entire DC Network
Significance Of Absorption Spectra For The Chemotaxonomic Characterization Of Pigmented Bacteria, Nuretti̇n Şahi̇n
Significance Of Absorption Spectra For The Chemotaxonomic Characterization Of Pigmented Bacteria, Nuretti̇n Şahi̇n
Turkish Journal of Biology
A method for characterizing pigmented bacteria based on the numerical analysis of their carotenoid absorption spectra was examined. To achieve this, some non-phototrophic pigmented bacteria that were isolated from various environments and closely related types of species were used. Bacterial carotenoid pigments were extracted with an acetone:methanol (7:2) mixture and the absorption spectra were determined. Data were normalized, and their first derivatives were analyzed using Ward clustering algorithms. Based on the spectral data, isolates were separated into 7 groups. Characterization based on derivative pigment spectral data showed good agreement statistically with biochemical characterization results. The Mantel test indicated that the …
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Influence Of Process Parameters On The Production Of Detergent Compatible Alkaline Protease By A Newly Isolated Bacillus Sp. Y., Shivakumar Srividya, Majumdar Mala
Turkish Journal of Biology
Detergent compatible alkaline protease production by a newly isolated Bacillus sp.Y from laundry soil was studied under shake flask conditions in growth medium comprised (g/L): peptone, 1; NaCl, 5; skim milk, 100; Na_2CO_3, 4. Different environmental process parameters such as fermentation period, initial pH, incubation temperature, and nutritional parameters such as supplementation of different starch sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Rice flour showed an almost 2.2-fold increase in protease activity followed by wheat flour, which showed a 1.85-fold increase over the basal media. However, supplementation of potato starch, corn starch, and ragi …
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Turkish Journal of Biology
The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu's) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico-chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen …
Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen
Possible Seasonal Variation Of The Fatty Acid Composition From Melanopsis Praemorsa (L., 1758) (Gastropoda: Prosobranchia), From Southeast Anatolia, Turkey, İhsan Eki̇n, Mehmet Başhan, Ridvan Şeşen
Turkish Journal of Biology
Possible seasonal variations of the fatty acid composition (neutral lipid, phospholipid, and total lipid content) of the freshwater snail Melanopsis praemorsa were investigated over the course of a year. Snail body lipids were fractionated by thin layer chromatography. Fatty acid composition of total body lipid, phospholipid, and neutral lipid of whole snail and total lipid of their algal food were analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. The most abundant fatty acids were C16:0, C18:0, C16:1\omega7, C18:1\omega9, C18:2\omega6, and C18:3\omega3 acids in the lipid analyses of the snail and in its food. Odd-numbered fatty acids and C20 polyunsaturated …
Influence Of Salinity On The Growth And Heavy Metal Accumulation Capacity Of Spirodela Polyrrhiza (Lemnaceae), Zeli̇ha Leblebi̇ci̇, Ahmet Aksoy, Fati̇h Duman
Influence Of Salinity On The Growth And Heavy Metal Accumulation Capacity Of Spirodela Polyrrhiza (Lemnaceae), Zeli̇ha Leblebi̇ci̇, Ahmet Aksoy, Fati̇h Duman
Turkish Journal of Biology
Given that the characteristic of free floating plants to uptake metals can have a positive effect on the efficiency of the remediation process of a wetland, it is important to investigate the factors that may impact the process of uptake of metals by the plants. By conducting this present study over a short duration of time, the effects of salinity on the growth, the content of the photosynthetic pigments (chlorophyll a, b, and carotenoid), and heavy metal uptake by Spirodela polyrrhiza were investigated. It was also observed that at a high level of salinity (100 and 200 mM NaCl) the …
Spectrum And Frequency Of Macromutations Induced In Chickpea (Cicer Arietinum L.), Aijaz A. Wani
Spectrum And Frequency Of Macromutations Induced In Chickpea (Cicer Arietinum L.), Aijaz A. Wani
Turkish Journal of Biology
The present investigation was undertaken to study the frequency and spectrum of morphological mutants induced by gamma rays and EMS in chickpea (Cicer arietinum L.). The seeds of 2 varieties of chickpea, Pusa-212 and Pusa-372, were treated with gamma rays (150 Gy, 200 Gy, 300 Gy, and 400 Gy), EMS (0.1%, 0.2%, 0.3%, and 0.4%) and combination treatments (200Gy+0.2%EMS, 300Gy+0.2%EMS, 200Gy+0.3%EMS, and 300 Gy+0.3%EMS). The M2 population was carefully screened for various viable macromutations. A wide spectrum of viable morphological mutations affecting almost all parts of the plant were isolated in the M2 generation. The most striking mutants isolated included …
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
Turkish Journal of Biology
Prevention and treatment of various degenerative diseases using traditional medicines is increasingly generating interest, especially in geriatric clinical research. This study aimed to evaluate the free-radical scavenging properties and potential to prevent DNA damage (from oxidative stress) of 56 extracts (polar and non-polar) from 14 medicinal plants (from 12 families) used by diverse Indian tribes and ethnic groups. Although widely known for traditional uses, these plants have not been extensively used for clinical purposes. Scavenging of DPPH^{\bullet} radical by all the extracts was evaluated to estimate their antioxidant potential and rank them accordingly. Extracts occupying the top 10 ranks were …
Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener
Evaluation Of Antibacterial, Antifungal, Antiviral, And Antioxidant Potentials Of Some Edible Oils And Their Fatty Acid Profiles, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Bi̇lge Şener
Turkish Journal of Biology
In the current study, the oils obtained from the nuts of Corylus avellana L. (hazelnut), Arachis hypogea L. (peanut, groundnut), Pinus pinea L. (umbrella nut), and Juglans regia L. (walnut) were tested for their antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and antimicrobial activity against the standard and isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, and Enterococcus faecalis as well as the fungi Candida albicans and C. parapsilosis by microdilution method, in which inhibition was expressed as minimum inhibition concentrations (MICs). Moreover, antiviral activity of the oils was determined using Herpes simplex (HSV) …
The Composition Of The Essential Oils Of Calamintha Pamphylica Subspecies, Sevi̇m Alan, Mi̇ne Kürkçüoğlu, K.H.C. Başer
The Composition Of The Essential Oils Of Calamintha Pamphylica Subspecies, Sevi̇m Alan, Mi̇ne Kürkçüoğlu, K.H.C. Başer
Turkish Journal of Biology
The essential oils obtained by hydrodistillation of the aerial parts of Calamintha pamphylica Boiss. & Heldr. subsp. pamphylica (A,B), subsp. davisii (Quezel & Contandr.) P.H.Davis (C,D,E), and subsp. alanyense S.Alan & Ocak (F) were analyzed using GC/MS. Menthyl acetate (26.2%-40.7%), menthol (13.5%-18.7%), and pulegone (7.7%-13.0%) were the main constituents in samples A and B; menthone (21.7%), pulegone (19.7%), and menthyl acetate (9.8%) were detected in sample C; isomenthyl acetate (16.7%), isomenthone (13.4%), and menthyl acetate (13.0%) were detected in sample D; menthyl acetate (20.9%), menthol (15.4%), and menthone (14.7%) were detected in sample E; and hexadecanoic acid (30.1%), menthyl acetate …
Molecular Diversity Of Thermophilic Bacteria Isolated From Pasinler Hot Spring (Erzurum, Turkey), Ahmet Adigüzel, Kadri̇ye İnan, Fi̇kretti̇n Şahi̇n, Tüli̇n Arasoğlu, Medi̇ne Güllüce, Ali̇ Osman Beldüz, Özlem Bariş
Molecular Diversity Of Thermophilic Bacteria Isolated From Pasinler Hot Spring (Erzurum, Turkey), Ahmet Adigüzel, Kadri̇ye İnan, Fi̇kretti̇n Şahi̇n, Tüli̇n Arasoğlu, Medi̇ne Güllüce, Ali̇ Osman Beldüz, Özlem Bariş
Turkish Journal of Biology
The present study was conducted to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Pasinler hot spring, Erzurum, Turkey. Fatty acid profiles, BOX PCR fingerprints, and 16S rDNA sequence data were used for the phenotypic and genotypic characterization of thermophilic bacteria. Totally 9 different bacterial strains were selected based on morphological, physiological, and biochemical tests. These strains were characterized by molecular tests including fatty acid and BOX profiles, and 16S rDNA sequence. The data of fatty acid analysis showed the presence of 14 different fatty acids in the 9 bacterial strains examined. Additionally, 5 of these fatty …
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
An Entomopathogenic Bacterium, Pseudomonas Putida, From Leptinotarsa Decemlineata, Hacer Muratoğlu, Zi̇hni̇ Demi̇rbağ, Kazim Sezen
Turkish Journal of Biology
The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was …
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
Turkish Journal of Biology
The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Turkish Journal of Biology
A total of 45 lactic acid bacteria were isolated from 10 different boza samples consumed in Turkey. The isolates were identified with respect to colonial, cellular morphology, and biochemical characters based on API CHL50 (Biomérieux) kit and also automated riboprinting was used to identify of the isolates. These isolates were tested for inhibitory activity against food-borne bacterial pathogens. Antimicrobial effects of these lactic acid bacteria were determined by the agar spot test and also agar diffusion method. As a result of the RiboPrinter® Microbial Characterization System (DuPont Qualicon) lactic acid bacteria isolates having antimicrobial activity (45 isolates) were identified as …
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Turkish Journal of Biology
Gas metal arc welding is a widely used method in a lot of industrial areas as it is cheap and produces high quality results. In the present study, peripheral blood lymphocytes of 23 welders and 25 non-exposed subjects were monitored for cytotoxicity. Information regarding the subjects' ages, smoking habits, alcohol consumption, duration of exposure, and medicine usage was recorded. According to the results, mitotic index (2.278 ± 1.325) of the welders was higher than that of those non-exposed (0.956 ± 0.616) (P < 0.01). There was no significant difference between mitotic index rates of smoking and non-smoking subjects. In contrast, there were significant differences between non-exposed subjects and welders for both smoking and non-smoking (P < 0.05). It was found that as the time of exposure to welding fumes increased so did the mitotic index rates (P < 0.05). Both a positive correlation in welders and a negative correlation in non-exposed subjects were observed between mitotic index frequency and age. In the light of the results obtained, it is advised that welders who work with gas metal arc welding be instructed and necessary protective measures be taken because of the risk caused by metal arc welding fumes.
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Turkish Journal of Biology
DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the …
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Turkish Journal of Biology
In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) …
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
Turkish Journal of Biology
The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Turkish Journal of Biology
Prangos species are widely used as medicinal plants in Turkey, and 14 species of this genus grow naturally in Anatolia. In the present study, the phenolic contents and antioxidant activities of the water and methanol (MeOH) extracts obtained from the root, herb, and fruits of 4 species of Prangos (Prangos ferulacea, P. heyniae, P. meliocarpoides var. meliocarpoides, and P. uechtritzii) collected in Konya province were compared. The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. Antioxidant activities of the extracts were studied by qualitative DPPH^. (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and by …
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Turkish Journal of Biology
The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Turkish Journal of Biology
Antioxidant activities in the leaves and fruits of wild Vaccinium arctostaphylos (locally named Qare-Qat) from different regions of Iran were evaluated using the stable 1, 1-diphenyl-2-picrylhydrazyl free radical and ferric reducing/antioxidant power methods. Variations in antioxidant activity, total phenolic, anthocyanin, and flavonoid content in fruit samples among genotypes was much greater than the variation observed between growing seasons, indicating that genetics plays a more important role than growing season in V. arctostaphylos fruits. The data indicated that the fruits of the Kelardasht genotype had the highest total phenolic content [42.73 ± 1.5 mg gallic acid equivalents (GAE) g^{-1} DW], anthocyanin …
Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun
Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun
Turkish Journal of Biology
The aerial parts and seeds of Sophora alopecuroides L. var. alopecuroides growing in Turkey were investigated for their alkaloid compositions and antimicrobial activities. The alkaloid extracts were analysed by capillary gas chromatography-mass spectrometry (GC-MS). The main components were identified as sophoridine (33.07%), matrine (24.03%), and sophocarpine (8.01%) in the aerial parts, and matrine (36.67%) and sophoridine (34.53%) in the seeds of the plant. The alkaloid extracts were also evaluated for their in vitro antibacterial and antifungal activities using microdilution techniques. The alkaloid extract of the aerial parts of S. alopecuroides var. alopecuroides presented significant activity against Staphylococcus aureus and Bacillus …
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Turkish Journal of Biology
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Turkish Journal of Biology
RNA interference (RNAi) pathway is a gene silencing process during which small double-stranded RNA (dsRNA) molecules trigger the degradation of homologous RNA targets. Small interfering RNAs (siRNAs) are the mediators of the RNAi that can be induced in vitro and in vivo by direct application of chemically synthesized siRNAs. Recently, promoter-targeted small non-coding RNAs have been described to be capable of regulating gene expression at the transcriptional level. In the present study we tested the hypothesis that Dicer-substrate siRNAs (D-siRNAs), which trigger gene silencing through intrinsic RNAi pathway and are therefore more potent in gene knockdown than traditionally used 21-23mer …
Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇
Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Biology
A gene encoding \beta-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 °C. The enzyme was found to be stable at 40 °C; however, 12% of LicA activity was lost at …
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
Turkish Journal of Biology
: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be …
Effects Of Cold And Salicylic Acid Treatments On Nitrate Reductase Activity In Spinach Leaves, Beli̇nda Aydin, Barbaros Nalbantoğlu
Effects Of Cold And Salicylic Acid Treatments On Nitrate Reductase Activity In Spinach Leaves, Beli̇nda Aydin, Barbaros Nalbantoğlu
Turkish Journal of Biology
The effects of long-term cold (5/3 °C) and salicylic acid (SA) (0.1 mM) treatments on the diurnal pattern of nitrate reductase (NR) activity in spinach (Spinacia oleracea L. cv. Gladiator) leaves were investigated. When the activities with cold treatment are compared to the control (20/15 °C), NR activity was decreased by 5 °C in the light period and by 3 °C in the dark period. The cold treatment also affected the timing of circadian rhythm in the diurnal pattern of NR activity. When the activities with SA treatments are compared to control and cold, it was determined that SA increases …
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Turkish Journal of Biology
We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 \muM) and naphthalene acetic acid (NAA, 10 \muM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. The application of PPM and antibiotics at different concentrations reduced contamination and led to …
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
Turkish Journal of Biology
To evaluate possible interactions between the renin-angiotensin system (RAS) and the sympathetic nervous system (SNS) in the systemic circulation upon the intravenous administration of angiotensin II and a set of adrenergic agonists in normal Sprague-Dawley (SD) rats. Rats were treated with carvedilol, a non-selective \beta- and selective \alpha_1-adrenoceptor blocker, in a dose of (5 mg/kg) orally for 7 days, and on day 8, the animals were pentobarbitone-anaesthetized and prepared for systemic hemodynamic study. Dose-response relationships in terms of elevation in the magnitude of the mean arterial blood pressure in response to intravenous injection of noradrenaline (NA), phenylephrine (PE), methoxamine (ME), …
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Turkish Journal of Biology
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Turkish Journal of Biology
In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …