Protective Immunization In Mice Against Group B Streptococci Using Encapsulated C5a Peptidase,
2013
University of Iowa
Protective Immunization In Mice Against Group B Streptococci Using Encapsulated C5a Peptidase, Donna Santillan, M. E. Andracki, S. K. Hunter
Donna A. Santillan
OBJECTIVE: The purpose of the study was to test whether C5a peptidase encapsulated within a biodegradable polymer can act as a vaccine and elicit an immune response to prevent group B streptococci (GBS) infection in mice and provide protection to pups. STUDY DESIGN: C5a peptidase was encapsulated in semipermeable microspheres of poly(lactide-co-glycolide). Female ICR mice were immunized with encapsulated C5a peptidase, free C5a peptidase, or empty microparticles. Booster doses were given at days 21 and 42. Antibody responses were measured by enzyme-linked immunosorbent assay. Challenge with GBS type III was performed 4 days after the final booster in the vaginal …
Efficacy Of Polymeric Encapsulated C5a Peptidase-Based Group B Streptococcus Vaccines In A Murine Model,
2013
University of Iowa
Efficacy Of Polymeric Encapsulated C5a Peptidase-Based Group B Streptococcus Vaccines In A Murine Model, Donna Santillan, K. Rai, Mark Santillan, Y. Krishnamachari, A. Salem, S. Hunter
Donna A. Santillan
OBJECTIVE: The purpose was to examine in mice the efficacy of various polymeric-encapsulated C5a peptidase vaccine formulations in eliciting a long-term immune response and preventing group B streptococcus (GBS) infection. STUDY DESIGN: C5a peptidase was encapsulated in semipermeable microspheres of poly(lactide-coglycolide) (PLGA). Female ICR mice were immunized with 0, 10, or 30 mug of encapsulated C5a peptidase within 2 different formulations of PLGA polymers. Booster doses were given at weeks 4 and 8. Antibody responses were measured by enzyme-linked immunosorbent assay at weeks 4, 8, 11, and 40. Vaginal challenges with GBS types 1a, III, and V were performed at …
The Mll Fusion Gene, Mll-Af4, Regulates Cyclin-Dependent Kinase Inhibitor Cdkn1b (P27(Kip1)) Expression,
2013
University of Iowa
The Mll Fusion Gene, Mll-Af4, Regulates Cyclin-Dependent Kinase Inhibitor Cdkn1b (P27(Kip1)) Expression, Z. Xia, R. Popovic, J. Chen, C. Theisler, T. Stuart, Donna Santillan, F. Erfurth, M. Diaz, N. Zeleznik-Le
Donna A. Santillan
No abstract provided.
Indoleamine 2,3 Dioxygenase (Ido) Deficiency Results: In Vascular Dysfunction And Proteinuria In Pregnancy,
2013
University of Iowa
Indoleamine 2,3 Dioxygenase (Ido) Deficiency Results: In Vascular Dysfunction And Proteinuria In Pregnancy, Mark Santillan, Christopher Pelham, Pimonrat Ketsawatsomkron, Donna Santillan, Baoli Yang, Stephen Hunter, Curt Sigmund
Mark K. Santillan
No abstract provided.
Cell Encapsulation As A Potential Nondietary Therapy For Maternal Phenylketonuria,
2013
University of Iowa
Cell Encapsulation As A Potential Nondietary Therapy For Maternal Phenylketonuria, Donna Santillan, Mark Santillan, Stephen Hunter
Mark K. Santillan
OBJECTIVE: The objective of this work was to determine whether cells overexpressing phenylalanine (Phe) hydroxylase (PAH) can significantly reduce Phe in vitro for potential use as a therapy for preventing maternal phenylketonuria. STUDY DESIGN: Human 293T and WRL68 cell lines were transiently and stably transfected to overexpress PAH. Cells were encapsulated within microspheres of sodium alginate. Timed measurements of Phe in media were performed using tandem mass spectrometry. RESULTS: Both nonencapsulated and encapsulated transiently transfected cells overexpressing PAH significantly reduced the Phe concentration in media by approximately 50% in comparison to mock-transfected cells. Cell line clones stably expressing PAH significantly …
"A Day In The Life:" A Simulated Experience,
2013
University of Texas at Tyler
"A Day In The Life:" A Simulated Experience, Melinda Hermanns, Narisa Waldo, Mary Luanne Lilly
Nursing Faculty Publications and Presentations
An experiential learning activity titled, "A Day in the Life" was implemented with nineteen baccalaureate nursing students in order to achieve a holistic understanding of the challenges faced by persons living with Schizophrenia and a physical disability, such as a fracture in an upper extremity or a visual impairment. The role play simulation required that students interact with public transportation and community resources, while assuming the role of a person with Schizophrenia and a physical disability. Using a qualitative descriptive methodology, reflective journals, aesthetic expressions, and post conference discussions about "A Day in the Life" were reviewed and analyzed by …
Metabolic Syndrome And Depression: A Systematic Review Of The Association,
2013
Brigham Young University
Metabolic Syndrome And Depression: A Systematic Review Of The Association, Michael S. Robinson
Student Works
Purpose: To explore whether there is an association between metabolic syndrome (MetS) and depression, the nature of the relationship, and implications for practicing health care professionals. Data Sources: Peer reviewed articles obtained through electronic database search in: CINAHL, The Cochrane Library, MEDLINE, and PsychINFO between the years of 2006 and 2013. Results: MetS and depression have a bidirectional association; MetS leads to depression and vice-versa. More severe depression has a stronger association with MetS. Increased waist circumference (AKA: abdominal obesity, body mass index [BMI]), elevated triglycerides, and low HDL cholesterol are the MetS components most commonly/strongly associated depression. Though not …
Conference Learnings,
2013
Lehigh Valley Health Network
Conference Learnings, Kimberly Bartman Rn, Bsn, Cvn
Patient Care Services / Nursing
No abstract provided.
Selective Discontinuation Of Luteal Phase Support In Assisted Reproduction Cycles Using A Gonadotropin-Releasing Hormone Agonist And Human Menopausal Gonadotropins For Ovarian Stimulation.,
2013
University of Iowa
Selective Discontinuation Of Luteal Phase Support In Assisted Reproduction Cycles Using A Gonadotropin-Releasing Hormone Agonist And Human Menopausal Gonadotropins For Ovarian Stimulation., D. Stovall, Bradley Van Voorhis, Amy Sparks, L. Adams, C. Syrop
Bradley J Van Voorhis
No abstract provided.
Tubal Versus Uterine Transfer Of Cryopreserved Embryos: A Comparative Analysis.,
2013
University of Iowa
Tubal Versus Uterine Transfer Of Cryopreserved Embryos: A Comparative Analysis., Bradley Van Voorhis, D. Stovall, Amy Sparks, Craig Syrop
Bradley J Van Voorhis
No abstract provided.
Clinical Factors Associated With Live Birth After Single Embryo Transfer,
2013
University of Iowa
Clinical Factors Associated With Live Birth After Single Embryo Transfer, Jessica Kresowik, Amy Sparks, Bradley Van Voorhis
Amy E.T. Sparks
No abstract provided.
Clinical Factors Associated With Live Birth After Single Embryo Transfer Editorial Comments,
2013
University of Iowa
Clinical Factors Associated With Live Birth After Single Embryo Transfer Editorial Comments, Jessica Kresowik, Amy Sparks, Bradley Van Voorhis
Amy E.T. Sparks
No abstract provided.
Helping New Nurses Be Assertive About Patient Safety,
2013
University of Iowa
Helping New Nurses Be Assertive About Patient Safety, S. Kinsella, K. Bigelow, S. Romano, Amy Sparks
Amy E.T. Sparks
No abstract provided.
Gamete Intrafallopian Transfer Pregnancy Rates Are Not Affected By Use Of Propofol Anesthesia.,
2013
University of Iowa
Gamete Intrafallopian Transfer Pregnancy Rates Are Not Affected By Use Of Propofol Anesthesia., T. Mukherjee, Y. Bellin, L. Andres, R. Vincent, Amy Sparks
Amy E.T. Sparks
No abstract provided.
Decreased Expression Of The Heat Shock Protein Hsp70-2 Is Associated With The Pathogenesis Of Male Infertility,
2013
University of Iowa
Decreased Expression Of The Heat Shock Protein Hsp70-2 Is Associated With The Pathogenesis Of Male Infertility, H. Feng, J. Sandlow, Amy Sparks
Amy E.T. Sparks
OBJECTIVE: To examine the expression of the heat shock protein hsp70-2, and the possible relationship with the pathogenesis of male infertility. DESIGN: Prospective study. SETTING: Reproductive testing laboratory in a university hospital. PATIENT(S): Men undergoing testicular biopsy during an investigation of subfertility. INTERVENTION(S): Testicular tissues were obtained from biopsies of men undergoing infertility evaluation and subdivided into three groups: normal testes, maturational arrest and Sertoli cell-only syndrome. Immunostaining and Western blotting techniques determined expression of the heat shock protein hsp70-2 MAIN OUTCOME MEASURE(S): Expression of the heat shock protein hsp70-2 in the testes. RESULT(S): The experimental data demonstrated that the …
Gene-Transfer Efficiency During Gestation And The Influence Of Co-Transfer Of Non-Manipulated Embryos On Production Of Transgenic Mice,
2013
University of Iowa
Gene-Transfer Efficiency During Gestation And The Influence Of Co-Transfer Of Non-Manipulated Embryos On Production Of Transgenic Mice, R. Canseco, Amy Sparks, R. Page, C. Russell, J. Johnson, W. Velander, R. Pearson, W. Drohan, F. Gwazdauskas
Amy E.T. Sparks
Litter size of DNA microinjected zygotes is lower than for non-manipulated zygotes. The rate of embryonic and fetal survival in early, mid and late gestation was determined to assess whether DNA integration was responsible for embryonic losses. Also, the effect of including non-microinjected embryos with injected embryos on pregnancy rate and transgenic pup production was determined. In Experiment I, one-cell embryos from immature CD-1 mice were microinjected with a whey acidic protein promoter-human protein C gene construct. One hour after microinjection embryos were transferred to pseudopregnant recipients (45 transfers of 30 embryos each). Fifteen recipients were sacrificed on day 4, …
Semen Quality In Fertile Men In Relation To Psychosocial Stress,
2013
University of Iowa
Semen Quality In Fertile Men In Relation To Psychosocial Stress, A. Gollenberg, F. Liu, C. Brazil, E. Drobnis, D. Guzick, J. Overstreet, J. Redmon, Amy Sparks, C. Wang, S. Swan
Amy E.T. Sparks
OBJECTIVE: To examine the association between stressful life events and semen parameters. DESIGN: Cross-sectional analysis in a pregnancy cohort study. SETTING: Prenatal clinics in five U.S. cities. PATIENT(S): Fertile men (n = 744) in the Study for Future Families, a cohort study of pregnant women and their partners. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Sperm concentration, percent motile, and percent normal morphology and classification above/below World Health Organization (WHO) cutoffs for semen quality. RESULT(S): After adjusting for confounders, men reporting 2+ recent stressful life events had an increased risk of being classified below WHO thresholds for "normal" defined by concentration, motility, …
Selective Discontinuation Of Luteal Phase Support In Assisted Reproduction Cycles Using A Gonadotropin-Releasing Hormone Agonist And Human Menopausal Gonadotropins For Ovarian Stimulation.,
2013
University of Iowa
Selective Discontinuation Of Luteal Phase Support In Assisted Reproduction Cycles Using A Gonadotropin-Releasing Hormone Agonist And Human Menopausal Gonadotropins For Ovarian Stimulation., D. Stovall, Bradley Van Voorhis, Amy Sparks, L. Adams, C. Syrop
Amy E.T. Sparks
No abstract provided.
Culture Of One-Cell Bovine Embryos In Explanted Mouse Oviduct And Bovine Oviductal Epithelial Cells,
2013
University of Iowa
Culture Of One-Cell Bovine Embryos In Explanted Mouse Oviduct And Bovine Oviductal Epithelial Cells, Amy Sparks, F. Gwazdauskas, M. Mcgilliard
Amy E.T. Sparks
One-cell bovine embryos fertilized in vivo were cultured in TCM-199 and bovine oviductal epithelial cells, in TCM-199, or in explanted immature mouse oviducts supported by TCM-199 to compare development to the blastocyst stage. The morphological stage of development and cell number were determined following 144 hours of culture. Of the embryos that cleaved at least once, 52.6, 30.4 and 0.0% developed to the morula/blastocyst stage after culture in oviductal epithelial cells, in TCM-199 alone, or in explanted mouse oviducts, respectively. The mean total cell number for embryos cultured in oviductal epithelial cells (24.5) was higher than for embryos cultured in …
Serum Inhibin-B In Fertile Men Is Strongly Correlated With Low But Not High Sperm Counts: A Coordinated Study Of 1,797 European And Us Men,
2013
University of Iowa
Serum Inhibin-B In Fertile Men Is Strongly Correlated With Low But Not High Sperm Counts: A Coordinated Study Of 1,797 European And Us Men, N. Jorgensen, F. Liu, A. Andersson, M. Vierula, D. Irvine, J. Auger, C. Brazil, E. Drobnis, T. Jensen, P. Jouannet, J. Overstreet, J. Redmon, Amy Sparks, J. Toppari, C. Wang, N. Skakkebaek, S. Swan
Amy E.T. Sparks
OBJECTIVE: To describe associations between serum inhibin-b and sperm counts, adjusted for effect of time of blood sampling, in larger cohorts than have been previously reported. DESIGN: Cross-sectional studies of spermatogenesis markers. SETTING: Four European and four US centers. PATIENT(S): Fertile men (1,797) were included and examined from October 1996-February 2005. INTERVENTION(S): The study was observational and therefore without any intervention. MAIN OUTCOME MEASURE(S): Associations between inhibin-b and semen variables controlled for time of blood sampling and other covariates. RESULT(S): Inhibin-b decreased about 2.00% per hour from 8 am-12 pm and then about 3.25% per hour from 12 pm-4 pm. …
