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Full-Text Articles in Experimental Analysis of Behavior
Application Of The “Idisco” Full-Brain Tissue Clearing Method: A View Of Dopamine And Inflammation In The Hiv-1 Transgenic Rat, Kristin Nickole Kirchner
Application Of The “Idisco” Full-Brain Tissue Clearing Method: A View Of Dopamine And Inflammation In The Hiv-1 Transgenic Rat, Kristin Nickole Kirchner
Theses and Dissertations
The iDISCO (immunolabeling-enabled three-dimensional imaging of solvent-cleared organs) method is a quick, inexpensive, and easily adaptable tissue staining and clearing procedure that allows neuroscientists to study a protein of interest in a whole, unaltered tissue sample. While the iDISCO method was initially tested and validated for mice embryos and brains, the current experiment sought to adapt the method for use in the rat using HIV-1 transgenic (Tg) rat brain tissue. Antibodies for tyrosine hydroxylase and Iba-1 were validated in the HIV-1 Tg rat and in F344/N control rats using iDISCO. Confocal images were taken of tyrosine hydroxylase positive neurons in …