Forensic Science and Technology Commons^™

Evaluating A Test For Shedding Propensity Using Tape Lifts From Different Skin Locations, Xiao M. Chen

Student Theses

The shedding propensity of a person can assist data interpretation in casework when assessing the possibility of passive transfer for DNA analysis. Past studies on shedding propensity evaluated palmar skin (washed and unwashed) deposits. This study compared different skin locations with respect to shedding propensity, and explored the potential of tape-lifts as a skin surface collection method. Eight different skin types and samples were collected with adhesive tape disks from 28 participants over three non-consecutive days; the washed and unwashed fingers from both hands, toe, and arm, neck below ear, and nape. Samples were extracted, quantified, amplified, genotyped, and evaluated …

Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson

Student Theses

Contact traces are an important part of DNA casework, but the probative value of any identified associations depends on the possibility of passive transfer. There is known individual variation in DNA left behind during contact, this DNA shedding propensity has an effect on whose DNA is detected. This study evaluated this variability using a cell staining approach. Volunteers were asked to deposit a fingerprint on a clean glass slide, then wash their hands and deposit a second fingerprint after a 30-minute wait without touching anything. Three sets of samples were collected over three consecutive weeks. Fingerprints were stained with a …

The Understanding Of Digital And Multimedia Evidence (Dme) By Attorneys And Digital Forensic Examiners (Dfe) Within The United States Criminal Justice System, Joseph Levi White

Dissertations

One goal of this research was to determine potential themes that may influence the understanding of Digital and Multimedia Evidence (DME) by attorneys and Digital Forensic Examiners (DFE) within the United States Criminal Justice System. Qualitative semi-structured interviews were conducted to gather information from experienced criminal attorneys and DFEs regarding potential influences on their understanding of DME. The results of these interviews were transcribed, and the data coded to allow for qualitative analysis. Five themes were developed from this data and are thought to play a role in understanding of DME by attorneys and DFEs: motivation for involvement in the …

Detecting Gsr Indicative Particles On Decayed Bones Using A Novel Field Kit, Sven Engling

Student Theses

Decomposed human remains are complex forensic puzzles, escalating in difficulty as the remains’ age obscures evidence, like trauma. Research has shown that scanning electron microscopes with energy dispersive X-ray spectrometers (SEM-EDX) are capable of detecting and identifying gunshot residue (GSR) particles on bones. However, SEM-EDX work is time consuming, expensive, and not accessible to every forensic department. Therefore, a preliminary field test capable of detecting GSR indicative particles, like lead, could save departments money and assist in trauma identification. This study examines the viability of using either the 3M Lead Check Test swabs or a sodium rhodizonate solution as part …

Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney

Student Theses

In this research, bovine leg bones were burned, both with and without meat intact, in order to determine if it was possible to extract and amplify DNA to obtain profiles. The meat was burned over an open flame and the DNA was extracted using the Qiagen DNeasy Blood & Tissue Mini Kit, quantified with the NanoDrop, and amplified using the Bovine Genotypes Panel 3.1 Kit, and separated using the 3500 Genetic Analyzer. Profiles were analyzed using the Thermo Fisher Cloud Microsatellite Analysis software. The samples burned with the meat intact produced lower quantities of DNA than the bones burned with …

Effects Of Improper Ammunition Storage, Victoria S. Andre

Student Theses

Firearms are commonly used by law enforcement, hunters, and civilians either for protection, sport or criminal activity. Ammunition is used in traditional firearms such as rifles and handguns, and consists of a cartridge case, propellant, a bullet (projectile), and primer. The two types of priming systems within the cartridge that are commonly used today are rimfire and centerfire cartridges. There are several types of malfunctions that can occur with ammunition. Improper ammunition storage is one major factor that plays a role in ammunition failure. One form of improper ammunition storage is where the storage environment being used abruptly changes due …

Integration Of Blockchain Technology Into Automobiles To Prevent And Study The Causes Of Accidents, John Kim

Electronic Theses, Projects, and Dissertations

Automobile collisions occur daily. We now live in an information-driven world, one where technology is quickly evolving. Blockchain technology can change the automotive industry, the safety of the motoring public and its surrounding environment by incorporating this vast array of information. It can place safety and efficiency at the forefront to pedestrians, public establishments, and provide public agencies with pertinent information securely and efficiently. Other industries where Blockchain technology has been effective in are as follows: supply chain management, logistics, and banking. This paper reviews some statistical information regarding automobile collisions, Blockchain technology, Smart Contracts, Smart Cities; assesses the feasibility …

The Influence Of Demographic Information On Public Attitudes Towards Individuals Who Commit Sexual Offenses, Emily Bogdan

Student Theses

Research exploring the factors that shape public attitudes towards individuals who commit sexual offenses is needed to inform policy and reduce stigma that these individuals face as they reenter society. Prior research has explored demographic factors of those who offend and have been victimized, but few have studied how these variables may interact with one another to shape attitudes toward people who commit sexual offenses. The current study explores whether offender gender, victim gender, and victim age shape the public’s attitudes towards these individuals. Participants were presented with a vignette describing the offense and then they were asked to respond …

Evaluation Of The Potential Of Automated Sem-Eds Analysis For The Discrimination Of Inorganic Soil Particles, Anna S. Duggar

Dissertations, Theses, and Capstone Projects

Soil, that complex mixture of minerals, organic particles, chemicals, and anthropogenic materials, is ubiquitous and easily transferred, which gives it forensic relevance. Forensic soil analysis has traditionally included the identification and comparison of both organic and inorganic components, the inorganic portion being most commonly examined by light microscopy and other instrumental techniques. Predominantly, the inorganic materials in soil are naturally-occurring minerals, contributed as grains from the surrounding bedrock, which gives a soil its regional character. That parent material then undergoes physical, chemical, and anthropogenic changes producing wide geographic variation within a single region. This variation forms the basis for potential …

Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis

Student Theses

Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …

Bullet Ricochet Of Polymer Coated Bullets, Stephen Daly

Student Theses

Polymer coated bullets were designed to limit a shooter’s exposure to lead. However, despite the advantages that these bullets give there are still difficulties which arise in a forensic firearms examination. Unlike in a typical metal coated bullet, individualizing striations will typically not be imparted to a polymer coated bullet making it unable to be linked to a specific firearm. Due to this difficulty any information that can be gained at the scene of the crime could be crucial. Some obtainable information includes the angle of ricochet and the angle of incidence of the discharged bullet as well as traces …

Investigation Of Postmortem Methamphetamine Cases Submitted To The New York City Office Of Chief Medical Examiner, Isaiah Jewell

Student Theses

Methamphetamine (N-methylamphetamine) is a central nervous system stimulant (CNS) and sympathomimetic drug with a high addiction potential. In the United States, there has been a significant increase in the presence of methamphetamine in recent years, specifically in the Northeastern region of the country. The New York City Office of Chief Medical Examiner (NYC-OCME) postmortem methamphetamine casework from 2018 and 2019 was analyzed and revealed that the presence of the drug increased drastically within a span of one year, jumping from 65 cases in 2018 to 99 cases in 2019. Males were overwhelmingly responsible for much of the casework, taking up …

Amino Acid Composition Analysis As A Means To Differentiate Hair Samples From Individuals Of Similar Demographics And The Effect Of Hair Treatments, Trisha Brady

Honors Theses

Hair is a forensically-relevant exhibit due to its ability to be shed, torn, or transferred during a crime. Current forensic hair analysis includes microscopic hair comparison and mitochondrial or nuclear DNA analyses, each with limitations. Novel methods, such as those evaluated herein, have potential to complement conventional techniques. These include evaluating functional groups with infrared (IR) spectroscopy, elements with laser induced breakdown spectroscopy (LIBS), and amino acid ratios with gas chromatography- mass spectrometry (GC-MS). Hair from two demographically similar individuals was divided into three sample groups per individual: untreated, bleached, and dyed. Spectral differences between individuals and treatments were evaluated …

Evaluation Of The Effect Of The Humic Acid Inhibitor On Forensic Genetic Investigations Of Human Skeletal Remains, Madeline King

Honors Theses

Postmortem survival of DNA in human skeletal remains occurs due to the compact microstructure of the skeleton and its ability to provide a strong, protective physical barrier to environmental insults. On a molecular level, DNA preservation in bones/teeth involves electrostatic interactions between the negatively-charged DNA backbone and positively charged calcium residues in hydroxyapatite, the latter of which is one of the main components of bone microstructure. Despite these protections, over time endogenous DNA becomes damaged, limiting our ability to detect it and affecting its utility in making a positive identification. Hence, forensic genetic investigations of unidentified human remains (UHRs) are …

Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez

Honors Theses

Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …

Investigating The Use Of Saligae® To Identify Expirated Blood On Various Material, Maria Bigos

Honors Theses

Expirated bloodstains and impact bloodstains have very similar physical characteristics. The main way Blood Pattern Analysts identify bloodstains in based on their physical characteristics. This can lead to difficulty distinguishing between expirated blood and impact spatter. Expirated blood can be identified through the presence of air bubbles, however these are often only present on non-porous surfaces. However, if expirated bloodstains can be identified through the presence of saliva, this can provide an alternative way to identify expirated bloodstains. This study examined if SALIgAE can be accurately used to identify the presence of saliva in blood and thus, be a way …

Criterion Validity Of The Ohio State University Traumatic Brain Injury Identification Method In A Criminal Justice Sample, Sarah M. Monaghan

Student Theses

The purpose of the current study was to determine the clinical utility of the Ohio State University Traumatic Brain Injury Identification Method (OSU-TBI-ID) in a criminal justice sample, by evaluating the criterion-related validity of this instrument. It was hypothesized that this tool could differentiate between incarcerated individuals with or without a history of Traumatic Brain Injury (TBI) on measures evaluating important TBI-related sequalae. The sample consisted of 95 incarcerated men detained at a private correctional facility in a Mid-Atlantic state. Measures used in this study to evaluate executive functioning difficulties, psychiatric difficulties, substance use problems, institutional misconduct and recidivism were …

Determination Of Human Shedding Propensity Based On Str Results, Genevieve Trapani

Student Theses

Trace DNA evidence may be discovered at a crime scene after having been deposited by a person of interest via active or passive transfer. Based on previous studies, passive transfer of one’s DNA is influenced by their shedding propensity, or probability of depositing a detectable amount of DNA through touch. Determining the shedding propensity of a person of interest can aid in trace DNA interpretation in forensic casework. This study explored STR profile quality and the presence of a DNA mixture for different skin surface locations, including fingertips before and after handwashing. As expected, unwashed fingers showed a higher prevalence …

Correlation Between Tooth Root Translucency, Crystallinity, And Dna Preservation In Teeth For Unidentified Human Remains (Uhr) Investigations, Paul Yount

Master's Theses

Endogenous genetic material protected by the rigid, mineralized hard tissues of bones and teeth is often targeted for forensic DNA testing in missing persons and unidentified human remains (UHR) investigations. Although standard approaches have narrowed sample selection to a few optimal skeletal elements (i.e., weight-bearing long bones, teeth), subsampling and decisions beyond this can be challenging due to the non-uniform, heterogeneous pattern of postmortem diagenesis. One particular component of skeletal microstructure — the inorganic mineral matrix [hydroxyapatite (HAp), Ca10(PO4)6(OH)2] — is purported to play a major role in DNA preservation. The potential correlation between molecular changes to skeletal microstructure and …

Unraveling The Mummy: The Effects Of Natural Mummification On The Recovery And Degradation Of Dna, Marissa Gandolfo-Muller

Honors Theses

As demonstrated through casework and research studies (Hawass et al., 2010; Gielda & Rigg, 2017), anthropogenic mummification and modern-day embalming can expedite degradation of DNA. Current research in the field of forensic mummification is sparse and little research has been done on quantifying naturally mummified DNA (Leccia et al., 2018; Shved et al., 2014). This research focuses on observing and quantifying the differences in the recovery and degradation of DNA from specimens that have been naturally mummified. This research on natural, forensic mummies is a blend of experimental archeology and postmortem DNA analysis.

In this study, two control specimens and …

Investigation Of Palatal Rugoscopy For Forensic Human Identification, Cameron Filipe

Honors Theses

Human identification is critical to the field of forensic science as well as in many sub-disciplines, including forensic pathology, forensic anthropology, and forensic odontology. While few widely-accepted methods of identification dominate the discipline (primarily fingerprint and dental record analysis), the study of palatal rugae patterns (rugoscopy) is an emerging tool in human identification. Some studies suggest that patterns of the palatal rugae are an individualizing characteristic among humans. While the use of rugoscopy for identification has been explored as an identifying marker in the dental and orthodontic communities, its use for forensic applications is not widely recognized. Also, while some …

The Opportunities And Challenges Of Internet Of Things Evidence In Regard To Criminal Investigations, Nathan Mcclaran

Honors Theses

As internet-connected devices become more prevalent, the need for police and law enforcement to be able to utilize these devices when solving crimes steadily increases. Law enforcement agencies are already aware of the opportunities and challenges when interacting with computers and mobile cellular devices, but many agencies are unable to utilize these avenues of evidence because of limited access to resources able to forensically examine computing devices. In addition, the introduction of internet of things devices, such as Amazon Alexa and other microphone or camera connected devices, allows new opportunities for law enforcement agencies to gather digital forensic evidence either …

How To Get Away With Murder: The Effectiveness Of Blood Detectors On Surfaces Cleaned Of Blood, Grace Cavanaugh

Undergraduate Theses and Capstone Projects

The purpose of this experiment was to test blood detectors against household cleaners. Similar experiments have been run before, but not on the selected surfaces. It is important to understand what one is looking for at a crime scene, and what a cleaned surface may look like. Four surfaces were chosen for this experiment: ceramic tile as a control, concrete, laminated wood flooring, and drywall. For each surface, 0.5mL of pig’s blood was allowed to dry before being cleaned off with either Clorox bleach, Dawn soap and hot water, a Mr. Clean Magic Eraser, or Mrs. Meyer’s Multi-Purpose Concentrate. Bluestar …

Cultivation Effects From Television Crime Dramas To Forensic Practices, Stephanie P. Mobley

Honors Theses

Television and its effects have been frequently studied due to the prevalence of television in today’s society. Cultivation theory examines these effects by claiming that people’s perceptions of the real world are shaped by the social reality portrayed on television. This paper used this theory to examine a link between college student’s viewership of television crime dramas and their opinions about forensic practices. It used quantitative survey questions and previous research to come to conclusions for the research questions. The three research questions asked whether frequent viewing of crime dramas cultivated false beliefs about three forensic practices, (fingerprint analysis, polygraph …

Effect Of Age And Sex On The Discrimination Of Monozygotic Twins Using Cg18562578 Dna Methylation In Saliva, Carla Pierre Louis

Student Theses

DNA methylation is a type of epigenetic modification that impacts gene expression without disturbing the genetic nucleotide sequence. DNA methylation has the potential to be included in the daily forensic analysis because among other uses, it has the potential to be applied to discrimination of monozygotic (MZ) twins that currently cannot be differentiated via forensic DNA profiling. In previous research, our group showed that DNA methylation at the cg18562578 site in chromosome 3 could be used to discriminate MZ twins. Because studies have shown that DNA methylation measures can be influenced by demographic factors such as sex and age; here, …

The Efficacy Of Recovering Latent Fingerprints From Fired Cartridge Cases By Way Of Cyanoacrylate Fuming Combined With Basic Yellow Dye Staining, Lily Mantz

Theses and Dissertations

This experiment attempted to tackle the long-standing struggle to consistently recover latent fingerprints from fired cartridges. With an emphasis on realistic handling of high volume of samples combined with Cyanoacrylate fuming followed by Basic Yellow dye staining, a total of 740 cartridges were examined. These included fired and unfired brass and nickel case 9mm Luger and 40 S&W cartridges. The cartridges were divided into five different groups; each were treated differently prior to cyanoacrylate fuming and dye staining, which was conducted in the same manner for the groups. The groups included the positive and negative controls, loaded, cycled, and fired. …

Powerplex® Fusion 6c System Versus Powerplex® Fusion 5c: A Comparison Of Performance Metrics, Caitlin M. Mccaughan

Theses and Dissertations

Following the FBI mandated expansion of the CODIS core loci from 13 to 20, several manufacturers developed short tandem repeat (STR) typing kits in response to the new criteria. One such manufacturer was the Promega Corporation, which released the PowerPlex® Fusion 5C megaplex STR typing kit (Fusion 5C) in 2012. Currently, the Virginia Department of Forensic Science (VDFS) utilizes this amplification kit for both casework and database applications. In 2015, Promega released the PowerPlex® Fusion 6C STR typing kit (Fusion 6C), which contains three additional loci beyond those included in PowerPlex® Fusion 5C, and a sixth color channel. The power …

Analysis Of Lubricants At Trace Levels Using Infrared Spectroscopy, Tanmai Bandarupalli

Honors Undergraduate Theses

Analysis of trace evidence involved in sexual assault investigations holds considerable potential as a newer avenue of identification when bulk, larger evidence is not found or unreliable. Trace analysis of forensic materials involves common findings such as strands of hair, residues left on clothing, shards of paint or glass, etc. In recent research focused on the analysis of trace materials found as evidence in a sexual assault, there has been promise in condom and bottled lubricant classification based on their chemical profiles that can provide an associative link in an investigation. Few studies have considered the examination of lubricant evidence …