Forensic Science and Technology Commons^™

Effect Of 17a - Ethynylestradiol On The Pupation Rate, Emergence Rate, And Sex Ratio Of Phormia Regina (Calliphoridae), Bethany K. Hoschar

Honors Theses

After death, blowflies are attracted to decomposing remains for feeding and reproduction purposes. Since blowflies are usually the first species to colonize a set of remains, they are important to forensic scientists when estimating the time since death. Using the knowledge of the life cycle of a blowfly and any interferences of the environment that can affect the development, forensic entomologists can estimate the time since the arrival of blowflies, otherwise considered time since deposition. This research investigated how a specific estrogen, 17 a-ethynylestradiol, present in pollutants affects the development time of blowflies, and therefore the time since death estimation. …

Fabricated Forensics: Examining An Undergraduate Population’S Ability To Detect Fallacies In Crime-Based Media, Conner Davis

Senior Capstone Theses

My research examines the effects of general education on students’ perspectives of the CSI effect. The CSI effect is a phenomenon in which people’s perceptions of criminal investigation are distorted from the truth because of the media’s portrayal of criminal investigation. The study sample includes undergraduate students enrolled in a Mid-Atlantic University. To quantify the degrees in which subjects are susceptible to the CSI effect, the subjects will be measured on their ability to identify basic forensic investigation flaws portrayed in three different television series. Subjects were given a worksheet, exposed to a fifteen-minute video compilation, and were told to …

Evaluation Of Dna From Blood And Saliva Overtime, Janine Smalling

Honors Theses

In this research, we will examine the quantity of DNA over time. In John M. Butlers, "Fundamentals of Forensic DNA Typing", he charts out the average amount of DNA extracted from different bodily fluid samples. Examples include blood, semen, saliva, urine, etc. Though valuable, the values presented are in the cases of a fresh extraction of the sample. It is important to note that in most cases when DNA is being extracted from crime scene evidence, the samples are not fresh and have been degraded to some extent. On a crime scene, time is only one of the factors affecting …

Effects Of Mercury On The Dopamine Transporter Cell Surface Expression In Pc12 Cells, Christina Hui

Student Theses

Environmental factors, such as heavy metal exposures, have been suggested to have an impact not only on neurodegenerative disease, such as Parkinson’s disease, but also on psychostimulants abuse and their toxicity. In this study, two questions were addressed: 1) effects of mercuric chloride on parkinsonian toxicant 1-methyl-4-phenylpyridinium (MPP⁺) induced cytotoxicity and 2) effects of mercuric chloride on cell surface dopamine transporter. Pheochromocytorma cells (PC12) were treated with various concentrations of mercuric chloride (0.02~2.0 ppm) for 4 hours with and without 0.1 mM MPP⁺. Significant potentiation of toxicity was observed when there was co-treatment with 0.5 ppm …

Evaluation Of A Novel Dna Collection Matrix Designed To Improve Recovery Of Touch Dna From Non-Porous Surfaces, Eric Ramirez

Student Theses

The collection of touch DNA from evidence has been a challenge in the field of Forensic Biology for decades. There are numerous collection protocols, substrates, and solutions that have been used to varying levels of success. Currently, no single method or substrate stands out as the single most effective collection protocol. The Gene Link Omni Matrix™ K105 solution is a novel collection method that can be sprayed onto the surface of non-porous evidence. The matrix dries into a film, capturing cells and cell-free DNA which can be scraped off and collected into a tube. During extraction, the matrix dissolves into …

Dna Mixture Statistics Using A Likelihood Ratio Software Tool: Effect Of Variations In Drop-Out Rates And Number Of Contributors, Felicia M. Lucero

Student Theses

Complex DNA mixtures can be very probative evidence, but comparisons to a person of interest can be affected by allelic drop-out and uncertainty regarding the number of individuals having contributed DNA to a sample. Scientific organizations such as the International Society of Forensic Genetics (Gill et al., 2006) recommend that likelihood ratios should be used to provide a statistical weight when a positive association is made between the DNA profile of a person of interest and an evidentiary DNA sample. To this effect the New York City Office of Chief Medical Examiner (OCME) developed a software program, Forensic Statistical Tool …

A Microspectra Analysis Of Synthetic Wig Fibers, Jaclyn R. Beshlian

Student Theses

The goal of this study was to determine whether differentiation between 20 visually similar light brown synthetic wig fibers was possible with the use of a microspectrophotometer (MSP). Visually similar fibers were chosen to display the power of the instrument over the human eye. An MSP is an instrument that has the ability to aid in the analysis of certain microscopical samples. With this particular instrument one is able to collect the absorption and fluorescence spectra of microscopical colored samples without destroying the piece of evidence itself. Prior to any analysis with the MSP, the material of the fibers was …

Dna Recovery From Handwritten Documents Using A Novel Vacuum Technique, Patrick M. Mclaughlin

Student Theses

Investigations of many crimes such as robberies, kidnappings, and terrorism are often associated with the recovery of a paper document which has been written by the perpetrator. Paper can provide a variety of forensic evidence such as DNA, latent fingermarks, and indented writing. The focus of this study was the collection of probative DNA profiles from the text region of a handwritten note through a vacuum suction device without altering or destroying the document. Collection of DNA evidence was carried out in two separate groups. The first group involved 11 volunteers providing a handwritten note sample with unwashed hands. The …

An Exploration Of Protein And Dna Components In Fingerprint Residue, Ashley Borrego

Student Theses

The main focus of this project was to investigate the protein and DNA components in both sebaceous and eccrine fingerprints. This study investigated the relative content of DNA and proteins in eccrine fingerprints to sebaceous fingerprints. All volunteers were instructed to wash and dry their hands prior to depositing parallel thumbprints. Twenty volunteers were instructed to touch their face to produce sebaceous prints, and 5 volunteers were instructed to wear gloves over a heat source to produce sweaty or eccrine prints. Microscopy was used to score the cellular debris of the right fingerprint on a scale of 1-4 based on …

Ethical Implications Of Codis, Lucy Grogan

Masters Theses

CODIS stores and maintains numerous DNA profiles, and is used as a tool by the criminal justice system in order to help solve crime. Deoxyribonucleic Acid, or DNA for short, is the genetic material that an individual inherits from one’s parents (NIH, 2019). Certain portions of this genetic material are selected for use within the CODIS database due to their lack of medically relevant information. There is an immense amount of power associated with DNA and the CODIS database that it is held within, which allows for many ethical issues to arise. In order to create a usable and safe …

Method Development And Validation For The Determination Of Designer Benzodiazepines In Blood By Lc-Ms/Ms, Victoria Mei

Student Theses

The misuse of designer benzodiazepines, as an alternative to prescription benzodiazepines and for drug-facilitated sexual assaults, has emerged as a growing threat, due in part to the ease of purchasing these drugs on the internet at low prices. Causing concern for safety, is the lack of dosage information resulting in users self-medicating, often leading to unintended overdoses, coma, or death at higher doses. With limited published data regarding quantification of designer benzodiazepines in forensic cases, a method was validated for the determination of thirteen and screening for two designer benzodiazepines in blood, in addition to a limited number of common …

The Forensic Analysis Of Children's Temporary Tattoos, Erika Bravo

Student Theses

While children’s temporary tattoos have been produced since the 20th century, there are few scientific studies that have evaluated their physical and chemical characteristics. Therefore, this thesis is a preliminary study done to evaluate the composition of children’s temporary tattoos and consider the importance of such traces in a forensic content.

Colored regions in children’s temporary tattoos, such as red, pink, yellow and orange, were found to fluoresce with the use of an alternative light source while the use of microscopy led to the identification of different printing patterns between temporary tattoos. However, limitations in the ability to determine the …

The Forensic Analysis Of Adult Temporary Tattoos, Paige Cooper

Student Theses

Adult temporary tattoos have become more popular, yet there is very little research on them, especially regarding their use in forensics. This poses a problem because forensic investigators may not be able to distinguish a temporary tattoo from a real tattoo, possibly hindering their investigation. In this study, various types of adult temporary tattoos were examined and analyzed in attempts to identify and distinguish them, both from each other and from permanent tattoos. Infrared spectroscopy, specifically attenuated total reflection (ATR), proved to be more useful than ultra-violet visible light spectroscopy in terms of analyzing the samples. The natural adult temporary …

Quorum Blockchain Stress Evaluation In Different Environments, Daniel P. Mera

Student Theses

In today’s world, the Blockchain technology is used for different purposes has brought an increment in the development of different Blockchain platforms, services, and utilities for storing data securely and efficiently. Quorum Blockchain, an Ethereum fork created by JPMorgan Chase, has placed itself in one of the widely used, efficient and trustful Blockchain platforms available today. Because of the importance which Quorum is contributing to the world, it is important to test and measure different aspects of the platform, not only to prove how efficient the software can be but as well as to have a clear view on what …

Simultaneous Determination Of Fourteen Antipsychotic Drugs In Whole Blood By Solid Phase Extraction And Liquid Chromatography Tandem Mass Spectrometry, Theresa M. Dawe

Student Theses

Anti-psychotic drugs are commonly prescribed to patients to treat several mental conditions, such as bipolar, schizophrenia, and manic-depressive disorder. The analysis of anti-psychotic drugs in blood is a common practice in clinical and forensic toxicology, to monitor drug treatment (therapeutic drug monitoring) or to explain the cause of the impairment or intoxication in human performance and in postmortem cases. However, most of the current studies have been performed in plasma, and a limited number in blood. We developed and validated a method to confirm and quantify a panel of commonly prescribed anti-psychotic drugs in whole blood using solid phase extraction …

Developing And Validating A Fast And Accurate Method To Quantify 18 Antidepressants In Oral Fluid Samples Using Spe And Lc-Msms, Sanghee S. Shin

Student Theses

Antidepressant drugs are one of the most widely used medicines for treating major depressive disorders for long time periods. Oral fluid (OF) testing offers an easy and noninvasive sample collection. Detection of antidepressants in OF is important in clinical and forensic settings, such as therapeutic drug monitoring and roadside testing for driving under influence. We developed and validated a comprehensive liquid chromatography tandem mass spectrometry method for 18 antidepressants (amitriptyline, bupropion, citalopram, clomipramine, cyclobenzaprine, desipramine, desvenlafaxine, doxepin, duloxetine, fluoxetine, imipramine, mirtazapine, nortriptyline, paroxetine, sertraline, trazodone, trimipramine, venlafaxine) in oral fluid collected by Quantisal® oral collection devices. 0.5 mL of Quantisal® …

Multiple Contacts Of Drug Contaminated Fingermarks And Their Analysis With Raman Microspectroscopy, Victoria Deprimo

Master's Theses

This thesis research aimed to determine if substrate, enhancement technique, and multiple contacts affect the detection and identification of drugs in fingermarks using Raman Spectroscopy. It has the potential to be of great importance in forensic science as fingermarks are one of the most important traces left behind at crime scenes and illicit drugs are a significant criminal justice problem. Thus, being able to associate illicit drugs with a specific fingermark has great potential for forensic science, as it can put the drugs in the hands of a specific individual.

The ridges of fingerprints trap trace amounts of material that …

Examining Witness Testimony In Domestic Homicides, Hana Chae

Student Theses

The present study investigated the effects of varying witness testimony on mock jurors’ perceptions of a case where a woman utilizes self-defense as a reason for killing her husband during a domestic dispute. A 3 (expert witness) x 3 (child witness) design was used to examine the effects of two different forms of expert testimony (Battered Woman Syndrome [BWS] & Social Agency [SA]) and its interaction with presence of child witness [age 5 & age 8]. Jury eligible participants (N = 245) were recruited from Amazon Mechanical Turk (MTurk). It was hypothesized that the presence of an expert witness would …

Methodological Improvements In The Mrna Profiling Assays For Incorporation Into Dna Casework Workflows, Paris Volk

Electronic Theses and Dissertations

Currently, DNA profiling is the gold standard to identify an individual. However, determining body fluid origin is important in criminal investigations, offering additional information surrounding the circumstances of a crime. However, crime labs can only definitively identify blood and semen and presumptively saliva using techniques that consume time and sample and do not simultaneously identify all forensically relevant body fluids. This causes many crime labs to want to bypass body fluid identification altogether. Therefore, advances into more definitive molecular-based body fluid methods are necessary. One such technique is mRNA profiling because it provides a highly sensitive and specific approach to …

Determining The Interference Of Lactate And Lactate Dehydrogenase In An Ethanol Enzyme Assay, Julia Liebl

Master's Theses

Enzymatic ethanol assays are widely used to determine blood alcohol content for individuals admitted to a hospital. In some instances, this hospital data is later used as evidence in a court of law, most commonly in DUI cases. As the enzymatic assay targets, but does not exclusively measure ethanol, it is theoretically possible that interference could occur and produce a falsely elevated result, leading to wrongful convictions or other consequences. This project examined the potential of clinically relevant levels of one potential interferant, lactate and the hepatic enzyme lactate dehydrogenase (LDH) to cause significant and falsely elevated results. Threshold levels …

Investigating The Masking Effects Of Blood On Organic Gunshot Residue Using Gc/Ms, Raman, And Atr-Ftir, Tyler Willits

Master's Theses

Gunshot residue (GSR) can be an indicator for determining the distance of a firearm discharge and linking potential suspects and witnesses to a shooting event. However, the evidentiary value of inorganic gunshot residue (IGSR), commonly used to detect GSR, has been called into question due to the potential for false negatives and false positives. Organic gunshot residue (OGSR) acts as supporting evidence; this increases the overall confidence of the examiner, as well as the value of gunshot residue evidence as a whole.

Current research has focused on three main areas for OGSR. The first is what compounds we should identify …

Dna Methylation Methods For Donor Age Prediction Using Touch Dna, Emily Neverett

Master's Theses

The International Labor Organization (ILO) estimated over 30 million individuals fall victim to human trafficking each year, of which, 50% are children below the age of 16. In 2012, the ILO reported there to be 168 million child laborers worldwide, with many trafficked into hazardous conditions to manufacture consumer products that are sold in developed countries. This is a modern form of slavery with poor working conditions, no access to education, and low wages. The hidden nature of this crime, however, makes it extremely difficult to identify and locate victims of forced child labor, and thus making it challenging to …

Forensic Characterization And Discrimination Of Manila Envelopes, María Isabel Sánchez Melo

Master's Theses

Envelopes can be found at crime scenes when ransom, threat letters or potentially harmful substances are sent to victims. Therefore, they are important probative items of evidence analyzed by forensic document examiners. Manila envelopes are commonly used in the United States to transport or send documents as they are made of thick and durable paper. Although there are many studies on the forensic analysis of office paper and paper-based banknotes, and only a few on white envelopes, there does not exist previously published research on the physical or chemical characterization of manila envelopes. The goal of this research was to …

The Forensic Analysis Of Skin-Safe Stamp Pad Inks, Swetha Rajagopal

Student Theses

Skin-safe stamp pad inks are currently being used at locations such as clubs, zoos and other events with the purpose of stamping individuals that visit the location. This is done in an attempt to monitor the number and activity of visitors. Ink manufacturing companies generally tend to experiment with a number of different combinations of the different components of ink, in order to obtain desirable properties. The combinations are usually proprietary and that very nature of the inks ends up making them, possibly unique and individual. The analysis, identification and possible individualization of the chemical composition of these inks could …

Detection Of Larval Aggregations Using A Drone Mounted Thermal Imaging Camera, Megan Descalzi

Master's Theses

When a body’s temperature reaches ambient temperature after death, remote detection of the body can become difficult. At this point, useful search tactics include the use of cadaver dogs, search parties and aerial imaging devices, all of which can be costly and time-consuming for every day law enforcement use. This study investigated the potential of a novel search technique in which a small, unmanned aerial system (drone) mounted with a forward-looking infrared radar (FLIR) was utilized to detect decomposing animal carcasses via the heat generated by associated Diptera larval aggregations. Hot water baths were utilized as analogs for larval aggregations …

Small Rna Sequencing And Rt-Qpcr Validation Of Forensically Relevant Body Fluids, Karly Johannsen

Master's Theses

Body fluid identification is an important aspect of forensic investigations because it assists with the reconstruction of crime scenes and can refute and/or support witness statements. Currently, there is no universal method for body fluid identification. Each body fluid has several tests for its identification, both presumptive and confirmatory. A universal method for body fluid identification that is sensitive, specific, efficient, and minimally destructive is necessary.

In recent years miRNAs have been heralded as novel biomarkers for the identification of body fluids. Several research groups across the US and internationally have been formed to determine which miRNAs are suitable for …

Construction Of A Reference Allelic Ladder For An Odocoileus Str Multiplex, Jolene Strand

Master's Theses

Odocoileus is a genus of Cervidae (deer) consisting of white-tailed deer (Odocoileus virginianus, Zimmerman, 1780), mule deer (Odocoileus hemionus, Rafinesque, 1817) and blacktailed deer (Odocoileus hemionus hemionus, Rafinesque, 1817). Hunting Odocoileus is only legal with proper permits according to laws that dictate when, where, how, and what can be hunted. Anything outside the legal limits is considered to be poaching. A useful tool for investigating poaching cases is Short Tandem Repeat (STR) DNA analysis. This is commonly used in human forensic casework to link DNA evidence found at crime scenes to either victims or suspects. In poaching cases, it can …

Assay Development For The Identification Of Species And Bioactive Compounds Of Morinda Citrifolia (Noni) In Dietary Supplements By Rt-Qpcr, Dna Sanger Sequencing And Spectroscopy (Uv-Vis/Ftir), Ricardo L. Fernandez Walker

Master's Theses

Regulation standards for dietary supplements lack greatly. Due to this, fraud during the manufacturing of these products might be committed. Previous investigations on herbal dietary supplements have shown that the main ingredient was not present in most of the herbal supplements tested and that these products also contained other substances not stated in the label The purpose of this study was to develop a fluorescent qPCR assay for the identification of Morinda citrifolia herbal species in dietary supplements. Primers for genetic regions rbcL and matK of M. citrifolia were designed. The potential of novel matK primer showed discrimination potential …

Optimizing Methods For Separation Of Adhesive Tape From Fabrics And Obtaining Latent Prints From Adhesive And Non-Adhesive Sides, Elizabeth Vosburgh

Forensic Science Theses

Fingerprinting is a valued part of forensic science analysis. It has been around for decades, and has advanced with the passing of time. There have been numerous studies of the different ways analysts have encountered fingerprints in the field—but none on those deal with the removal of tape from fabric. To investigate this, eight fabric types (a cotton/polyester mix, spandex, denim, jeans, fleece, flannel, polyester, and vinyl), three commercially available tapes (duct tape, black electrical tape, and packaging tape), have been stuck together and separated with four different techniques (manual pulling apart, Un-Du commercial adhesive remover, liquid nitrogen, and a …

Investigating The Binding Of Fentanyl And Fentanyl Analogs To The Μ-Opioid Receptor, Marissa Anne Teske

Honors Theses

In 1960, Dr. Paul Janssen of the Janssen Company synthesized fentanyl. It is a rapid-acting analgesic that, unlike similar analgesics around that time, did not have negative cardiovascular effects. Fentanyl, and its derivatives, are strong μ-opioid receptor agonists. Rates of fatal opioid overdoses involving fentanyl have increases rapidly over the past two decades Due to these increasing concerns related to fentanyl and its derivatives, it is important to not only study fentanyl and its known derivatives, but to also study and understand potential new derivatives and how all these compounds interact with the opioid receptors. This study examined the interactions …