Forensic Science and Technology Commons^™

Structural Integrity And Stability Of Dna In Ionic Liquid And Near-Infrared Indolizine Squaraine Dye, Ember Yeji Suh

Honors Theses

Luminol, the most common presumptive test for blood at a crime scene, has multiple issues, such as false positive results with chemical agents, no luminescence due to “active oxygen” cleaning agents on bloodstains, and inability to penetrate textile materials. A combination of indolizine squaraine dye and ionic liquid (IL), or Dye Enhanced Textile Emission for Crime Tracking (DETECT), have shown potential to address these issues. The purpose of this study was to assess the binding mechanism of CG (1:1) and SO₃SQ dye to HSA and how the mechanism can explain the W214 fluorescence quenching effect and to determine …

Csi Effect: Exploring Impact Among Mississippi Lawyers, Jennie Odom

Honors Theses

The “CSI Effect” suggests that a growing number of jurors often have unrealistic expectations concerning the amount of forensic evidence which should be reasonably presented to convict an alleged criminal, and this misconception arises from watching heavily dramatized crime shows such as the CSI franchise. While many scholars have examined the CSI Effect, one critical perspective is often missing from the existing literature, that of the lawyers. Therefore, this project worked to explore the perspectives of both defense attorneys and prosecutors. Personal interviews were conducted to gain insight and perspective regarding the CSI Effect, whether these perspectives had impacted the …

Evaluation Of Raman Spectroscopy As A Screening Tool To Predict Dna Recovery Potential In Skeletonized Human Remains, Cody Silverman

Honors Theses

The purpose of this study is to determine if Raman spectroscopy can be used as a rapid and non-destructive screening tool to predict DNA recovery potential for bone, and to explore the correlation between the compositional microstructure of bone and preservation of DNA. The two primary structural components of bone are calcium hydroxyapatite and collagen. It is proposed that by analyzing bone using Raman spectroscopy, the presence, state, and distribution of hydroxyapatite and collagen can be measured, which may be useful in predicting if the sample is viable for DNA analysis. The ultimate goal of this study is to develop …

An Investigation Of The Effect Of Temperature And Humidity On The Formation Of Antemortem Root Banding, Mae Griffin

Honors Theses

Microscopical hair analysis has become a controversial practice within forensic science primarily due to issues related to interpretation, and specifically overstating the significance of an association. The validity of microscopical hair comparisons has suffered from intense scrutiny by the forensic community, with one criticism being a paucity of peer reviewed research. An often-unrecognized aspect of microscopical hair analysis is its use in providing reconstructive information based on identifiable features. One example of this is postmortem root band or PMRB. PMRB is the formation of a band slightly above the root bulb and below the skin surface on the hair of …

Effect Of Coastal Environments On The Post Mortem Interval Of A Pig Carrion: Implications For Human Decomposition, Morgan Hough

Honors Theses

Forensic taphonomy involves the use of forensic techniques to determine the post mortem interval of a corpse. There are many factors that are known to influence the rate of decomposition, such as the temperature, season, soil type, moisture potential of the soil, and presence of insects or other decomposers. Previous studies in this field have focused primarily on decomposition patterns in mountainous environments in terms of terrestrial habitats or strictly aquatic environments, leaving this particular aspect of coastal settings to be unexplored.

To study these effects, the superior portion of a female pig was buried in sandy soil in mid-October …

An Interdisciplinary Analysis Of Dehumanization During The Holocaust, Alissa Marie Davies

Honors Theses

The body of literature surrounding the Holocaust and dehumanization is extensive. Multiple theories have been proffered to try and explain the Holocaust’s causation, each with varying degrees of acceptance from the Jewish and global communities. Meanwhile dehumanization has been studied however often through the lens of a singular field. Despite the abundance of research on the Holocaust, the subject of perpetrators has had limited exploration as of yet. The present analysis seeks to examine the mechanics of dehumanization and how these mechanics could explain the behaviors behind the perpetration of the Holocaust and by extension, other genocides. This analysis will …

Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng

Honors Theses

Forensic dentistry has evolved from simple morphological investigations to genetic and molecular analysis. One of the most important techniques is DNA analysis which requires viable samples from an individual or crime site. The orofacial region can be a good source for DNA samples because the tissues can resist environmental, thermal, electrical, and mechanical challenges. Teeth are especially useful as a source of genetic material because the DNA located in the pulp is surrounded by protective mineral tissue. The DNA in the pulp tissue is currently accessed through destructive methods involving crushing, pulverization, and splitting the tooth. Unfortunately, destructive methods destroy …

Amino Acid Composition Analysis As A Means To Differentiate Hair Samples From Individuals Of Similar Demographics And The Effect Of Hair Treatments, Trisha Brady

Honors Theses

Hair is a forensically-relevant exhibit due to its ability to be shed, torn, or transferred during a crime. Current forensic hair analysis includes microscopic hair comparison and mitochondrial or nuclear DNA analyses, each with limitations. Novel methods, such as those evaluated herein, have potential to complement conventional techniques. These include evaluating functional groups with infrared (IR) spectroscopy, elements with laser induced breakdown spectroscopy (LIBS), and amino acid ratios with gas chromatography- mass spectrometry (GC-MS). Hair from two demographically similar individuals was divided into three sample groups per individual: untreated, bleached, and dyed. Spectral differences between individuals and treatments were evaluated …

Evaluation Of The Effect Of The Humic Acid Inhibitor On Forensic Genetic Investigations Of Human Skeletal Remains, Madeline King

Honors Theses

Postmortem survival of DNA in human skeletal remains occurs due to the compact microstructure of the skeleton and its ability to provide a strong, protective physical barrier to environmental insults. On a molecular level, DNA preservation in bones/teeth involves electrostatic interactions between the negatively-charged DNA backbone and positively charged calcium residues in hydroxyapatite, the latter of which is one of the main components of bone microstructure. Despite these protections, over time endogenous DNA becomes damaged, limiting our ability to detect it and affecting its utility in making a positive identification. Hence, forensic genetic investigations of unidentified human remains (UHRs) are …

Extraction Of Human Dna From Soil In A Simulated Clandestine Grave, Arely Joaly Parra Lopez

Honors Theses

Locard’s Exchange Principle states that “every contact leaves a trace.” The same principle applies when a perpetrator of a homicide decides to bury a victim in a clandestine grave. If a perpetrator originally buried a murder victim in haste to prevent discovery and then decided to later move the victim’s body to a remote location for disposal, decomposition fluids from the victim’s body (which contain cellular material and therefore DNA) would remain in the surrounding grave soil at the original burial site. It is possible that investigators could: 1) prove that a human body had once laid in that location …

Investigating The Use Of Saligae® To Identify Expirated Blood On Various Material, Maria Bigos

Honors Theses

Expirated bloodstains and impact bloodstains have very similar physical characteristics. The main way Blood Pattern Analysts identify bloodstains in based on their physical characteristics. This can lead to difficulty distinguishing between expirated blood and impact spatter. Expirated blood can be identified through the presence of air bubbles, however these are often only present on non-porous surfaces. However, if expirated bloodstains can be identified through the presence of saliva, this can provide an alternative way to identify expirated bloodstains. This study examined if SALIgAE can be accurately used to identify the presence of saliva in blood and thus, be a way …

Unraveling The Mummy: The Effects Of Natural Mummification On The Recovery And Degradation Of Dna, Marissa Gandolfo-Muller

Honors Theses

As demonstrated through casework and research studies (Hawass et al., 2010; Gielda & Rigg, 2017), anthropogenic mummification and modern-day embalming can expedite degradation of DNA. Current research in the field of forensic mummification is sparse and little research has been done on quantifying naturally mummified DNA (Leccia et al., 2018; Shved et al., 2014). This research focuses on observing and quantifying the differences in the recovery and degradation of DNA from specimens that have been naturally mummified. This research on natural, forensic mummies is a blend of experimental archeology and postmortem DNA analysis.

In this study, two control specimens and …

Investigation Of Palatal Rugoscopy For Forensic Human Identification, Cameron Filipe

Honors Theses

Human identification is critical to the field of forensic science as well as in many sub-disciplines, including forensic pathology, forensic anthropology, and forensic odontology. While few widely-accepted methods of identification dominate the discipline (primarily fingerprint and dental record analysis), the study of palatal rugae patterns (rugoscopy) is an emerging tool in human identification. Some studies suggest that patterns of the palatal rugae are an individualizing characteristic among humans. While the use of rugoscopy for identification has been explored as an identifying marker in the dental and orthodontic communities, its use for forensic applications is not widely recognized. Also, while some …

The Opportunities And Challenges Of Internet Of Things Evidence In Regard To Criminal Investigations, Nathan Mcclaran

Honors Theses

As internet-connected devices become more prevalent, the need for police and law enforcement to be able to utilize these devices when solving crimes steadily increases. Law enforcement agencies are already aware of the opportunities and challenges when interacting with computers and mobile cellular devices, but many agencies are unable to utilize these avenues of evidence because of limited access to resources able to forensically examine computing devices. In addition, the introduction of internet of things devices, such as Amazon Alexa and other microphone or camera connected devices, allows new opportunities for law enforcement agencies to gather digital forensic evidence either …

Cultivation Effects From Television Crime Dramas To Forensic Practices, Stephanie P. Mobley

Honors Theses

Television and its effects have been frequently studied due to the prevalence of television in today’s society. Cultivation theory examines these effects by claiming that people’s perceptions of the real world are shaped by the social reality portrayed on television. This paper used this theory to examine a link between college student’s viewership of television crime dramas and their opinions about forensic practices. It used quantitative survey questions and previous research to come to conclusions for the research questions. The three research questions asked whether frequent viewing of crime dramas cultivated false beliefs about three forensic practices, (fingerprint analysis, polygraph …

Investigating The Efficacy Of Dna Damage With Bleach In Forensic Laboratories And At Crime Scenes, Alyssa Tuccinardi

Honors Theses

Household/commercial bleach (6% NaOCl, sodium hypochlorite) degrades DNA through oxidative damage, production of chlorinated base products, and cleavage of DNA strands (breaking it into smaller and smaller fragments). The presence of these lesions significantly impacts the ability to generate a full genetic profile from an evidentiary sample. In fact, knowledge of the damaging effect of bleach on DNA is the basis for its use in forensic laboratories to clean workbenches and prevent cross-contamination of samples between cases. Additionally, bleach is used intentionally by criminals to clean up crime scenes and destroy DNA evidence. A previous study demonstrated that bleach has …

Photofading Of Black Dyes In Cotton And Polyester Fibers Resulting From Environmental Exposure To Sunlight, Maggie E. Mahaney

Honors Theses

Color is one of the most important properties analyzed during forensic fiber examinations due to its discriminatory power. Differing colors in fibers are usually sufficient grounds for exclusion, but in cases where a lengthy period has passed between crime commission and evidence recovery, the high probability of environmental degradation having altered the questioned fibers’ properties could lead to false exclusions. Photofading, the loss of color resulting from exposure to light, is a common form of degradation observed in fibers. Although well-known in the dyeing industry, it is not typically factored into forensic fiber examinations. Published photofading research has been limited …

Investigation Of The History Of Fingerprinting, Advancements In The Field, And Development Of Potential Methods That Could Improve The Detection Of Endogenous And Exogenous Drugs In Latent Prints, Kristen Malloy

Honors Theses

When someone thinks of fingerprinting, they are most likely going to picture how a latent print is matched to the fingerprint of a suspect based on ridge pattern analysis. However, there is much more information that can be obtained from a latent print. The work performed in this thesis focuses the detection of exogenous and endogenous drugs in latent prints. The experiments performed analyzed fingerprints from volunteers that were contaminated with one of three common painkillers: acetaminophen, acetylsalicylic acid (ASA), and ibuprofen. Three different instruments were tested for this purpose: MALDI-MS, ATR-FTIR, and LC-MS. Based on the results gathered, it …

Small Island Taphonomy In Western Australia, Courtney J. Newberry

Honors Theses

Forensic death investigations rely on postmortem interval estimations to establish a timeline surrounding a decedent’s death. Several methods are used, often together, to make such estimations. One of the main methods is the evaluation of the stage of decomposition; although decomposition follows the same general steps, the length of the process can vary by location and environmental factors. Animal scavenging can also impact decomposition by consuming flesh and impacting insect activity on the remains.

This study investigates the scavenging guilds and rate of decomposition of pig (Sus scrofa) legs on Rottnest Island, Australia. Three sites with unique environments were selected …

Analyses Of Prosecutorial Power And Discretion In Mississippi: Evaluating Proposals To Address Misconduct And Abuse, Lucy Pruitt

Honors Theses

This thesis seeks to create a policy proposal in order to address incidences of prosecutorial misconduct and abuse of discretion in the Mississippi criminal justice system. To do so, the author has summarized and analyzed seven criminal cases in which defendants have become victims of prosecutorial misconduct in order to shed light on the lack of prosecutorial accountability in the state’s criminal justice system. In an attempt to solve the problem, the author has developed a novel grading rubric in order to objectively and systematically analyze and evaluate previously proposed policy recommendations by legal experts and justice organizations. The successes …

Effect Of 17a - Ethynylestradiol On The Pupation Rate, Emergence Rate, And Sex Ratio Of Phormia Regina (Calliphoridae), Bethany K. Hoschar

Honors Theses

After death, blowflies are attracted to decomposing remains for feeding and reproduction purposes. Since blowflies are usually the first species to colonize a set of remains, they are important to forensic scientists when estimating the time since death. Using the knowledge of the life cycle of a blowfly and any interferences of the environment that can affect the development, forensic entomologists can estimate the time since the arrival of blowflies, otherwise considered time since deposition. This research investigated how a specific estrogen, 17 a-ethynylestradiol, present in pollutants affects the development time of blowflies, and therefore the time since death estimation. …

Evaluation Of Dna From Blood And Saliva Overtime, Janine Smalling

Honors Theses

In this research, we will examine the quantity of DNA over time. In John M. Butlers, "Fundamentals of Forensic DNA Typing", he charts out the average amount of DNA extracted from different bodily fluid samples. Examples include blood, semen, saliva, urine, etc. Though valuable, the values presented are in the cases of a fresh extraction of the sample. It is important to note that in most cases when DNA is being extracted from crime scene evidence, the samples are not fresh and have been degraded to some extent. On a crime scene, time is only one of the factors affecting …

Cannabinoid Conundrum: A Study Of Anti-Epileptic Efficacy And Drug Policy, Kennedy Dickson

Honors Theses

Cannabis is the most commonly used, cultivated, and trafficked drug worldwide. The use and acceptance of marijuana is evolving rapidly, as indicated by the volume of new State cannabis legislation across the U.S. Many of the changes in state laws have occurred without significant input from medical or scientific communities. Additionally, marijuana policy in the US is convoluted with significant inconsistencies between state and federal law. The status of marijuana as a Schedule I drug under the Controlled Substance Act creates numerous restrictions and issues that impact the industry as a whole. The most promising development has been the 2018 …

Development Of A Dart-Mass Spectral Database For 3d-Printed Firearm Polymers, And Airborne Mercury At Three Lakes In North Mississippi, Parker Ball

Honors Theses

This research focuses on two studies: a development towards a DART-mass spectral database for 3D-printed firearm polymers, and a test for airborne mercury concentrations at three lakes in North Mississippi. With the relatively recent developments in 3D-printing technology, 3D-printed firearms have become increasingly prevalent as they have become more accessible to the public. Despite its growing popularity, little research has been done regarding the forensic analysis of evidence from 3D-printed firearms. Using DART-MS in conjunction with thermal desorption techniques, we obtained the mass spectra for 50 different commercially available 3D-printing polymers. Chemometric analysis was done to account for the high …

Investigating The Binding Of Fentanyl And Fentanyl Analogs To The Μ-Opioid Receptor, Marissa Anne Teske

Honors Theses

In 1960, Dr. Paul Janssen of the Janssen Company synthesized fentanyl. It is a rapid-acting analgesic that, unlike similar analgesics around that time, did not have negative cardiovascular effects. Fentanyl, and its derivatives, are strong μ-opioid receptor agonists. Rates of fatal opioid overdoses involving fentanyl have increases rapidly over the past two decades Due to these increasing concerns related to fentanyl and its derivatives, it is important to not only study fentanyl and its known derivatives, but to also study and understand potential new derivatives and how all these compounds interact with the opioid receptors. This study examined the interactions …

A Study Of The Kratom Alkaloids And Their Binding To The Μ-Opioid Receptor, Reba Elizabeth Ann Chamblee

Honors Theses

Kratom, a plant commonly found in southeast Asia, has traditionally been used for medicinal treatments. Recently, however, the popularity of the drug has significantly increased due to its euphoric effects, leading to it being used as an alternative to illegal opioids. Several alkaloid compounds have been isolated from the leaves of the plant. The main alkaloids seen are the following five alkaloids: mitragynine, 7-hydroxymitragynine, speciociliatine, speciogynine, and paynantheine. Two alkaloids, mitragynine and 7-hydroxymitragynine, have exhibited high potencies and are potentially even more potent than morphine. Previous studies have indicated that the main mediator of the psychoactive effects is the opioid …

Exploring Various Techniques To Process And Identify Latent Friction Ridge Details From Fingers On Semi-Porous Surfaces, Emily Campo

Honors Theses

Numerous terrorist groups in the Middle East have been recently making detonators for IEDs (Improvised Explosive Devices) using items as basic as flip flops. Because of the semi-porous nature of flip flops, military forensic response teams have been unable to find an effective method through which they can identify, process, and lift latent fingerprints from the surface of a flip flop. The present study aims to use three fingerprint principles to formulate a reliable method that can be used for such purposes. This study specifically investigated the potential use of powders and cyanoacrylate fuming as processing techniques. The case study …

Using Pattern Area Ridge Flow In The Three Areas Of The Palm To Determine Classification Trends, Ashton Horton

Honors Theses

Friction ridge skin is only located on the fingers, palms, and soles of feet. These ridges have been used to make identifications since before the twentieth century. These methods may be the basis of scientific testimony in court because of the amount of research that has been conducted in fingerprint identification. Courts uphold these techniques because of three principles behind fingerprint identification: fingerprints are unique because no two are the same, they will not change from the time they are formed in the womb until post-mortem decomposition, and they can be objectively classified using a general classification system. Little research …

A Validation Study Of Blueview™ Gunpowder Particle Testing Kit, Hanna Lester

Honors Theses

The examination of gunshot residue is often an integral part of evidence processing. The aim of this preliminary study is to validate the use of the BlueView Gunshot Particle Test Kit in the field. This was done by comparing the results of the presumptive tests using the BlueView Gunshot Particle Test Kit to confirmatory test results using a scanning electron microscope. This study used both collection techniques to collect samples for presumptive tests and confirmatory tests. Gunshot residue samples were collected from the hands after a series of guns were fired, and positive results were obtained from the presumptive and …

Analyzing Palm Print Shapes With Pressure And Distortion, Whitney Grannan

Honors Theses

While much research exists on latent fingerprints, there is a lack of research on palm prints. This study aims to discover how the variable of pressure affects the distortion of latent palm prints recovered from a crime scene. This study used various development techniques to extract prints from aluminum Coca-Cola cans, white copy paper, and household steak knives. The collected prints were scanned into the AFIX Tracker system and an auto-extract number, based on the number of minutiae points, was generated automatically from the system. These auto-extract numbers were then compared to a baseline, which consisted of an average of …