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Full-Text Articles in Forensic Science and Technology
High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood
High Resolution Melt Analysis Of Samples With Differential Dna Methylation To Identify Tissue Source Of Origin, Stephanie M. Ledgerwood
Master's Theses
In Forensic Science casework, identifying the source of a tissue can assist in crime scene reconstruction. Currently, presumptive testing methods to identify tissue type are utilized in crime scene laboratories, but there is a need for a more reliable confirmatory test for tissue type identification. High Resolution Melt (HRM) analysis is an innovative technology that has the potential to determine tissue types through variations in DNA methylation patterns. Recently, DNA methylation patterns have been found to correspond with specific tissue types in particular regions of DNA. Two markers, B_SPTB_03 and DDX4 have been effective in differentiating sperm from other tissue …
A Novel Method For Confirming The Presence Of Volatile Reduced Sulfide Compounds Via Inductively Coupled Plasma-Optical Emission Spectroscopy, Krystal Lynne Parker
A Novel Method For Confirming The Presence Of Volatile Reduced Sulfide Compounds Via Inductively Coupled Plasma-Optical Emission Spectroscopy, Krystal Lynne Parker
Master's Theses
Millions of dollars in destruction in the past decade have resulted from the use of Chinese drywall in homes. There are also potential health hazards related to this corrosive material. As such, it is important to find a way to identify Chinese drywall. Drywall can be tested for certain markers, such as strontium, sulfur, and carbonates to identify it as corrosive Chinese drywall. The laboratory preparation and analysis should be efficient and cost effective. The methods previously used, such as an X-ray fluorescence gun have had issues with getting a proper reading due to the layers of other materials found …
Sensitivity And Species Specificity Of Bisulfite Modification And Pyrosequencing Technology Used To Identify Tissue-Specific Dna Methylation Patterns In Common Biofluids Foudn In Crime Scenes, Sitara Christian Shirwani
Sensitivity And Species Specificity Of Bisulfite Modification And Pyrosequencing Technology Used To Identify Tissue-Specific Dna Methylation Patterns In Common Biofluids Foudn In Crime Scenes, Sitara Christian Shirwani
Master's Theses
In order to convict the guilty or exonerate the innocent in criminal cases, it is crucial to reconstruct the crime scene and/or determine the nature of the crime. Identifying the different biofluids found at a crime scene can help shed light on these aspects of forensic casework. Recently, DNA methylation has been used as a means of identifying biological materials, as opposed to conventional protein/enzyme based methods. DNA methylation is the addition of a methyl group to the 5' carbon on a cytosine base (C), which is directly followed by a guanine base (G) and are called CpG sites. The …
Comparison Of Liquid-Liquid Extraction And Solid Phase Extraction Of Mephedrone, Brittany Simone Love
Comparison Of Liquid-Liquid Extraction And Solid Phase Extraction Of Mephedrone, Brittany Simone Love
Master's Theses
Legal high drugs are modified scheduled drugs. As with any new drug, researchers have to conduct studies to gather information about the drug. The problem with obtaining accurate information on new drugs is that by the time information is gathered, drug abusers and street chemists have developed new ones. Comparing designer drugs to their illegal counterparts is often helpful in that it can provide a starting point. Mephedrone is a new designer drug that has become a problem over the past few years. Often marketed as bath salts and plant food, mephedrone has become the knockoff replacement for amphetamines. This …
Comparison Of Stated Iron Concentrations Between Name Brand And Generic Children's Multivitamins, Ashlea Nicole Pettengill
Comparison Of Stated Iron Concentrations Between Name Brand And Generic Children's Multivitamins, Ashlea Nicole Pettengill
Master's Theses
The mineral iron is one of the most common minerals on Earth; not only is iron found in the atmosphere, water, and plants, it is an essential mineral needed for the human body. Children need iron at a young age to ensure proper growth and development of myelin, to promote oxygen transportation within red blood cells, and to assist in Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA). With iron readily available in children's multivitamins, iron toxicity can become an issue when taking too many tablets. With two types of brands, generic and name brand, parents should be aware of the …
Single Liquid-Liquid Extraction Of Delta-9-Tetrahydrocannabinol And 11-Nor-Carboxy-Delta-9-Tetrahydrocannabinol At An Acidic Ph, Meghan Ann Tanner
Single Liquid-Liquid Extraction Of Delta-9-Tetrahydrocannabinol And 11-Nor-Carboxy-Delta-9-Tetrahydrocannabinol At An Acidic Ph, Meghan Ann Tanner
Master's Theses
Because of the high occurrence of marijuana usage in the workplace and in driving under the influence cases, it is important to test for both delta-9-THC and delta-9-THCA in order to determine current and recent usage. The laboratory preparation and analysis of delta-9-THC and delta-9-THCA in urine should be as cost and time efficient as possible. Current extraction of delta-9-THC and delta-9-THCA from urine requires two separate techniques for each. Because delta-9-THC is nonacidic, while delta-9-THCA is acidic, the extractions involve two separate pH levels, while GC/MS analysis requires two separate methods. This separation requires a great deal of time …
Capecitabine Induced Hand And Foot Syndrome And The Reproducibility Of Friction Skin, Rodney Allen Schenk
Capecitabine Induced Hand And Foot Syndrome And The Reproducibility Of Friction Skin, Rodney Allen Schenk
Master's Theses
In 2008, a 62 year old male was detained by United States Customs and Immigration officials when attempting to enter the United States because his fingerprints were not detectable. It was later reported by his medical doctor in Singapore that the individual suffered from Hand and Foot Syndrome (palmar-plantar erythrodysaesthesia) as a result of his cancer treatment of capecitabine (N4-pentyloxycarbonyl-5-deoxy-5- fluorocytidine) which causes interruptions to the normal growth of keratinocytes in the friction skin. Capecitabine is a recently developed, orally administered fluoropyrimidine prodrug designed to generate 5-fluorouracil through a three-step enzymatic process giving it antineoplastic properties to combat cancerous tumor …
Efficiency Of Using Commercial Dna Extraction Kits And The Organic Extraction Method In Removal Of Polymerase Chain Reaction Inhibitors, Brandi Jo Payne
Efficiency Of Using Commercial Dna Extraction Kits And The Organic Extraction Method In Removal Of Polymerase Chain Reaction Inhibitors, Brandi Jo Payne
Master's Theses
The first step in the determination of a perpetrator of a crime using DNA profiling is obtaining good quality DNA. The substrate on which the body fluid is located may contain substances that can co-extract with DNA and may inhibit subsequent PCR analysis. In this study, the efficiency of the removal of such contaminants were tested using three different methods, namely the PrepFiler DNA extraction kit, DNA IQ DNA extraction system, and the organic DNA extraction protocol. One, two, five, and ten μl of whole blood were deposited on soil, wood chips, and cotton swatches treated with bleach. DNA was …