Biological and Physical Anthropology Commons^™

Applications Of Pooled Dna Samples To The Assessment Of Population Affinities: Short Tandem Repeats, M. H. Crawford, P. Banerjee, D. A. Demarchi, M. Zlojutro, J. Mccomb, G. Livshits, M. Henneberg, M.J. Mosher, M. S. Schanfield, J. A. Knowles

Anthropology Faculty and Staff Publications

Pooled DNA samples have been used in association studies of Mendelian disease genes. This method involves combining equal quantities of DNA from patients and control subjects into separate pools and comparing the pools for distributions of genetic markers. In this study identical quantities of DNA from 300 individuals representing 6 populations were pooled and amplified for 296 loci using the touchdown polymerase chain reaction (PCR) method. The purpose of this study is to test the efficacy of pooled DNA markers in the reconstruction of the genetic structure of human populations. The populations sampled included Chuvash, Buryats, Kizhi, Native Americans, South …