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Full-Text Articles in Physics
Study Of Blinking Statistics In Silver Coated Cobalt Ferrite Single Nanoprticle Using Two-Photon Fluorescence Microscopy, Rajen Kumar Goutam
Study Of Blinking Statistics In Silver Coated Cobalt Ferrite Single Nanoprticle Using Two-Photon Fluorescence Microscopy, Rajen Kumar Goutam
Open Access Theses & Dissertations
Two-photon fluorescence microscopy is a powerful tool to study the molecular and cellular interactions. This technology is a non-invasive approach with the advantage of three-dimensional imaging up to submicron resolution. Two-photon excitation process is the result of simultaneous absorption of two photons that has special features of reduced photodamaged and elongated penetration depth on samples. In this work, blinking statistics of silver-coated Cobalt ferrite (CoFe2O4) single nanocrystal is studied using two-photon fluorescence microscopy. By defining the intensity threshold, the observed fluorescence is divided into two distinct stages: OFF and ON states. Both of these states followed the inverse power law. …
Non Linear Optics For Materials Fabrication And Medical Instrumentation, Aurelio Paez
Non Linear Optics For Materials Fabrication And Medical Instrumentation, Aurelio Paez
Open Access Theses & Dissertations
Two-photon absorption is a nonlinear optical process where two photons are absorbed by a molecule simultaneously. The probability of this quantum phenomenon is proportional to the quadratic excitation of light intensity. It has many applications in biomedical and materials research, such as two-photon fluorescence microcopy. The first project is to apply two-photon absorption induced bond cleavage in photoreactive materials for engineering 3D tissue scaffolds. The major challenge for growing thick 3D tissues is the lack of vasculature, where nutrients and oxygen can be delivered to the growing cells. Our collaborators have synthesized a novel polypeptide that is composed of 34-mer …
Using Two-Photon Microscopy To Analyze Arabidopsis Thaliana Leaves Under Autophagy Conditions And Second Harmonic Generation In Collagen, Andres Martin Reyes
Using Two-Photon Microscopy To Analyze Arabidopsis Thaliana Leaves Under Autophagy Conditions And Second Harmonic Generation In Collagen, Andres Martin Reyes
Open Access Theses & Dissertations
Two-photon microscopy has emerged as a highly efficient and effective imaging tool for biological samples. This can be utilized in studying recently emerging technologies and conditions such as the autophagy process in arabidopsis thaliana leaves and second harmonic generation in collagen. Both of these areas of study utilize the two-photon microscopy practice effectively in uncovering more information of the biological nature of examined samples.