Physics Commons^™

Remnant Polarization And Structural Arrangement In P(Vdf-Trfe) Electrospun Fiber Meshes Affect Osteogenic Differentiation Of Human Mesenchymal Stromal Cells, Bahareh Azimi, Massimiliano Labardi, Mohammad Sajad Sorayani Bafqi, Teresa Macchi, Claudio Ricci, Veronica Carnicelli, Lorenzo Scarpelli, Istiak Hussain, Francesca Matino, Mohammed Uddin

Physics and Astronomy Faculty Publications and Presentations

Highlights

• The type of solvent had noticeable effects on morphology and piezoelectric properties of P(VDF-TrFE) electrospun fibers.

• Using MEK as a solvent and specific environmental conditions led to the obtainment of surface nanopores.

• Uniaxially aligned fibers exhibited higher β phase and mechanical properties than random ones.

• Randomly oriented fibers had higher remnant piezoelectric properties (Vout, d31 piezoelectric coefficient) than aligned ones.

• Human mesenchymal stem cells cultured on randomly oriented fibers showed an accelerated osteogenic differentiation.

Abstract

Many tissues and cells are influenced by mechano-electric stimulation, thus the application of piezoelectric materials has recently received considerable attention in tissue engineering. …

Cell-Induced Confinement Effects In Soft Tissue Mechanics, Dawei Song, Jordan L. Shivers, Fred C. Mackintosh, Alison E. Patteson, Paul A. Janmey

Physics - All Scholarship

The mechanical properties of tissues play a critical role in their normal and pathophysiological functions such as tissue development, aging, injury, and disease. Understanding tissue mechanics is important not only for designing realistic biomimetic materials for tissue engineering and drug testing but also for developing novel diagnostic techniques and medical interventions. Tissues are heterogeneous materials consisting of cells confined within extracellular matrices (ECMs), both of which derive their structural integrity, at least in part, from networks of biopolymers. However, the rheology of purified reconstituted biopolymer networks fails to explain many key aspects of tissue mechanics. Notably, purified networks typically soften …

Orientation Distribution Of Highly Oriented Type I Collagen Deposited On Flat Samples With Different Geometries, Qamrun Nahar, David Minh Luan Quach, Behafarid Darvish, Harvey A. Goldberg, Bernd Grohe, Silvia Mittler

Physics and Astronomy Publications

The structural arrangement of type I collagen in vivo is critical for the normal functioning of tissues, such as bone, cornea, tendons and blood vessels. At present, there are no established low-cost techniques for fabricating aligned collagen structures for applications in regenerative medicine. Here, we report on a straightforward approach to fabricate collagen films, with defined orientation distributions of collagen fibrillar aggregates within a matrix of oriented collagen molecules on flat sample surfaces. Langmuir Blodgett (LB) technology was used to deposit thin films of oriented type I collagen onto flat substrates exhibiting various shapes. By varying the shapes of the …

Immunogold Labeling To Enhance Contrast In Optical Coherence Microscopy Of Tissue Engineered Corneal Constructs, Chris B. Raub, Elizabeth J. Orwin, Richard C. Haskell

All HMC Faculty Publications and Research

Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to the light scattering in the tissue. In order to do this, we are currently investigating the use of immunogold labeling. Gold nanoparticles are high scatterers and can create contrast in images. We have conjugated gold nanoparticles to …