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Technological University Dublin

Medicine and Health Sciences

Centrifugal filtration

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Full-Text Articles in Physics

Ultra-Filtration Of Human Serum For Improved Quantitative Analysis Of Low Molecular Weight Biomarkers Using Atr-Ir Spectroscopy, Franck Bonnier, Luis Felipe Cs. Carvalho, Matthew J. Baker, Hugh Byrne, Igor Chourpa Jun 2017

Ultra-Filtration Of Human Serum For Improved Quantitative Analysis Of Low Molecular Weight Biomarkers Using Atr-Ir Spectroscopy, Franck Bonnier, Luis Felipe Cs. Carvalho, Matthew J. Baker, Hugh Byrne, Igor Chourpa

Articles

Infrared spectroscopy is a reliable, rapid and cost effective characterisation technique, delivering a molecular finger print of the sample. It is expected that its sensitivity would enable detection of small chemical variations in biological samples associated with disease. ATR-IR is particularly suitable for liquid sample analysis and, although air drying is commonly performed before data collection, just a drop of human serum is enough for screening and early diagnosis. However, the dynamic range of constituent biochemical concentrations in the serum composition remains a limiting factor to the reliability of the technique. Using glucose as a model spike in human serum, …


Improved Protocols For Vibrational Spectroscopic Analysis Of Body Fluids, Franck Bonnier, François Petitjean, Matthew Baker, Hugh Byrne Mar 2014

Improved Protocols For Vibrational Spectroscopic Analysis Of Body Fluids, Franck Bonnier, François Petitjean, Matthew Baker, Hugh Byrne

Articles

The applications of vibrational spectroscopy to the examination of human blood serum are explored. Although FTIR spectra can be recorded in aqueous solutions at (gelatin) concentrations as low as 100mg/L, the high-wavenumber region remains obscured by water absorption. Using Raman spectroscopy, high quality spectra of gelatine solutions as low as 10mg/L can be achieved, also covering the high-wavenumber regions. In human serum, spectral profiles are weak and partially obscured by water features. Dried deposits are shown to be physically and chemically inhomogeneous resulting in reduced measurement reproducibility. Concentration of the serum using commercially available centrifugal filter devices results in an …