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Articles 1 - 8 of 8
Full-Text Articles in Physics
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Within condensed matter, single fluorophores are sensitive probes of their chemical environments, but it is difficult to use their limited photon budget to image precisely their positions, 3D orientations, and rotational diffusion simultaneously. We demonstrate the polarized vortex point spread function (PSF) for measuring these parameters, including characterizing the anisotropy of a molecule’s wobble, simultaneously from a single image. Even when imaging dim emitters (∼500 photons detected), the polarized vortex PSF can obtain 12 nm localization precision, 4°–8° orientation precision, and 26° wobble precision. We use the vortex PSF to measure the emission anisotropy of fluorescent beads, the wobble dynamics …
Imaging The Three-Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri-Spot Point Spread Function, Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew
Imaging The Three-Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri-Spot Point Spread Function, Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
Fluorescence photons emitted by single molecules contain rich information regarding their rotational motions, but adapting single-molecule localization microscopy (SMLM) to measure their orientations and rotational mobilities with high precision remains a challenge. Inspired by dipole radiation patterns, we design and implement a Tri-spot point spread function (PSF) that simultaneously measures the three-dimensional orientation and the rotational mobility of dipole-like emitters across a large field of view. We show that the orientation measurements done using the Tri-spot PSF are sufficiently accurate to correct the anisotropy-based localization bias, from 30 nm to 7 nm, in SMLM. We further characterize the emission anisotropy …
Diffusion Of Rubidium Vapor Through Hollow-Core Fibers For Gas-Phase Fiber Lasers, Eric M. Guild
Diffusion Of Rubidium Vapor Through Hollow-Core Fibers For Gas-Phase Fiber Lasers, Eric M. Guild
Theses and Dissertations
This work examines the diffusion of rubidium through a small diameter tube alone and in the presence of noble gases. A fluid dynamics analysis is investigated to aid in choosing a method for transferring atomic rubidium vapor that is reliable and efficient. Solutions to the time dependant ordinary differential equation describing the experimental flow properties of the system reveal more precise outcomes than previously practiced routines. Resolved viscosities and Poiseuille flow theory velocity profile distributions are characterized for noble gas carriers of the rubidium vapor. Applying Reynolds Numbers to the flow experiments provides pressure differential boundaries that are employed in …
Study Of The Photoinduced Surface Relief Modulation In Photopolymers Caused By Illumination With A Gaussian Beam Of Light, Tzvetanka Babeva, Dana Mackey, Suzanne Martin, Izabela Naydenova, Vincent Toal
Study Of The Photoinduced Surface Relief Modulation In Photopolymers Caused By Illumination With A Gaussian Beam Of Light, Tzvetanka Babeva, Dana Mackey, Suzanne Martin, Izabela Naydenova, Vincent Toal
Articles
The formation of surface relief profile in photopolymerisable systems when illuminated with a focused beam of light is simulated numerically using a two-way diffusion model that takes account both for monomer and short polymer chains diffusion. The concentration and spatial distribution dynamics of monomer and short and long polymer chains are calculated. The surface profile is obtained from calculated components concentrations considering different densities of monomer and polymer. The influence of the illumination time, intensity and spot diameter on the surface profiles dynamics is discussed. A good agreement between the calculated and the experimentally measured profiles is observed thus demonstrating …
Two Way Diffusion Model For The Recording Mechanism In A Self-Developing Dry Acrylamide Photopolymer, Suzanne Martin, Izabela Naydenova, Raghavendra Jallapuram, Robert Howard, Vincent Toal
Two Way Diffusion Model For The Recording Mechanism In A Self-Developing Dry Acrylamide Photopolymer, Suzanne Martin, Izabela Naydenova, Raghavendra Jallapuram, Robert Howard, Vincent Toal
Conference Papers
In our most recent study [1] diffusion constants were measured in the simplified monoacrylamide version of a dry acrylamide based photopolymer holographic recording material developed in the Centre for Industrial and Engineering Optics. In this paper we report diffusion constants for the commonly used photopolymer formulation, which also contains the crosslinker bisacrylamide. A physical model for the recording mechanism is proposed which explains the two way diffusion observed in both systems, and is in agreement with much of the previously observed behaviour of the material, particularly in regard to dependence of diffraction efficiency growth on spatial frequency and persistence of …
On The Use Of Variable Coherence In Inverse Scattering Problems, Erwan Baleine
On The Use Of Variable Coherence In Inverse Scattering Problems, Erwan Baleine
Electronic Theses and Dissertations
Even though most of the properties of optical fields, such as wavelength, polarization, wavefront curvature or angular spectrum, have been commonly manipulated in a variety of remote sensing procedures, controlling the degree of coherence of light did not find wide applications until recently. Since the emergence of optical coherence tomography, a growing number of scattering techniques have relied on temporal coherence gating which provides efficient target selectivity in a way achieved only by bulky short pulse measurements. The spatial counterpart of temporal coherence, however, has barely been exploited in sensing applications. This dissertation examines, in different scattering regimes, a variety …
Probing Random Media With Singular Waves, Chaim Schwartz
Probing Random Media With Singular Waves, Chaim Schwartz
Electronic Theses and Dissertations
In recent years a resurgence of interest in wave singularities (of which optical vortices are a prominent example), light angular momentum and the relations between them has occurred. Many applications in various areas of linear and non-linear optics have been based on studying effects related to angular momentum and optical vortices. This dissertation examines the use of such wave singularities for studying the light propagation in highly inhomogeneous media and the relationship to angular momentum transfer. Angular momentum carried by light can be, in many cases, divided in two terms. The first one relates to the polarization of light and …
Investigation Of The Diffusion Processes In Self-Processing Acrylamide-Based Photopolymer System, Izabela Naydenova, Raghavendra Jallapuram, Robert Howard, Suzanne Martin, Vincent Toal
Investigation Of The Diffusion Processes In Self-Processing Acrylamide-Based Photopolymer System, Izabela Naydenova, Raghavendra Jallapuram, Robert Howard, Suzanne Martin, Vincent Toal
Articles
Results from the investigation of the diffusion processes in a dry acrylamide-based photopolymer system are presented. The investigation is carried out in the context of experimental work on optimization of the high spatial frequency response of the photopolymer. Tracing the transmission holographic grating dynamics at short times of exposure is utilized to measure diffusion coefficients. The results reveal that two different diffusion processes contribute with opposite sign to the refractive index modulation responsible for the diffraction grating build up. Monomer diffusion from dark to bright fringe areas increases the refractive index modulation. It is characterized with diffusion constant D0=1.6E-7 cm2/s. …