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Full-Text Articles in Physics
Nanoaperture Fabrication Via Colloidal Lithography For Single Molecule Fluorescence Analysis, Ryan M. Jamiolkowski, Kevin Y. Chen, Shane A. Fiorenza, Alyssa M. Tate, Shawn H. Pfeil, Yale E. Goldman
Nanoaperture Fabrication Via Colloidal Lithography For Single Molecule Fluorescence Analysis, Ryan M. Jamiolkowski, Kevin Y. Chen, Shane A. Fiorenza, Alyssa M. Tate, Shawn H. Pfeil, Yale E. Goldman
Physics & Engineering Faculty Publications
In single molecule fluorescence studies, background emission from labeled substrates often restricts their concentrations to non-physiological nanomolar values. One approach to address this challenge is the use of zero-mode waveguides (ZMWs), nanoscale holes in a thin metal film that physically and optically confine the observation volume allowing much higher concentrations of fluorescent substrates. Standard fabrication of ZMWs utilizes slow and costly E-beam nano-lithography. Herein, ZMWs are made using a self-assembled mask of polystyrene microspheres, enabling fabrication of thousands of ZMWs in parallel without sophisticated equipment. Polystyrene 1 mu m dia. microbeads self-assemble on a glass slide into a hexagonal array, …
Correlations Between Short- And Long-Time Relaxation In Colloidal Supercooled Liquids And Glasses, Chandan K. Mishra, Xiaoguang Ma, Piotr Habdas, Kevin B. Aptowicz, A. G. Yodh
Correlations Between Short- And Long-Time Relaxation In Colloidal Supercooled Liquids And Glasses, Chandan K. Mishra, Xiaoguang Ma, Piotr Habdas, Kevin B. Aptowicz, A. G. Yodh
Physics & Engineering Faculty Publications
Spatiotemporal dynamics of short- and long-time structural relaxation are measured experimentally as a function of packing fraction, φ, in quasi-two-dimensional colloidal supercooled liquids and glasses. The relaxation times associated with long-time dynamic heterogeneity and short-time intracage motion are found to be strongly correlated and to grow by orders of magnitude with increasing φ toward dynamic arrest. We find that clusters of fast particles on the two timescales often overlap, and, interestingly, the distribution of minimum-spatial-separation between closest nonoverlapping clusters across the two timescales is revealed to be exponential with a decay length that increases with φ. In total, the experimental …