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Functional Characterization Of Calcium Dependent Protein Kinase 32 From Arabidopsis, Rucha Karve

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Calcium-dependent protein kinases (CPKs) are major plant Ca2+ sensors, many of which have roles in plant stress responses. The Arabidopsis genome encodes 34 CPK isoforms. Here we report characterization of AtCPK32 gene function. Analysis of transgenic plants expressing pCPK32-GUS shows that CPK32 is highly expressed in roots, pollen and embryo, as well as leaf hydathodes, and the abscission zone of mature siliques. Real time RT-PCR and promoter expression patterns show that CPK32 is responsive to abiotic and biotic stresses. Plants treated with salt, ABA, osmotic stress (PEG), wounding, and flagellin 22 peptide show up-regulation of CPK32 upon these stress treatments. …